ISAbo1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008260 | ND | Alcanivorax borkumensis | Alcanivorax borkumensis SK2 |
DNA section
IS Length : 1229 bp
Ends
IR Length : 30/37
IRL : TGAATCGCCACCAGTTTGCTAGATACCTTTTAGCCATACAAAATGGCAAC
IRR : TGAATCGCCACCAGTTCTCTAGACACTTCCCAGCCGTTCTTGGTGACGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTTTTTACA | AA | AACGTAACCC | 2 |
DNA sequence
TGAATCGCCACCAGTTTGCTAGATACCTTTTAGCCATACAAAATGGCAACCAGAGAGGTATCCATGAGCAGCAAACGCTATCCCGAAGAATTCAAGATTG
AGGCAGTTCGTCAGGTGACGGATCGTGGTCACAGTGTGGCCCAAGTGGCTGATCGTCTCGGTGTCACCACTCACAGTCTGTATGCCTGGATCAGGAAGTT
TGGCCCGGATTCCGAGCAGCATCAGGCCAATGCAGATGATCAAGTCGAGATTCGCCGACTCCAGAAAGAGCTCAAGCGCGTTACCGAAGAGCGAGACATC
CTAAAAAAAGCCGCGGCGTACTTCGCCAGTCAGTCCGATTGAGGTACGCCTTCATTCAGGAGCACAGCGAGCGGTGGGCAATTCGCTCACTGTGTTCCCT
GTTGGACGTTCACCCCAGTGGCTTTTATGCCTGGCAGAAACAGCCTCGTTCAGCTCGAGCAATCGAGGACGAGCGCCTGTCCGGGCTGATAAAGCAATTC
TGGTTGGAATCTGGAGCCGTGTATGGCTACAGGAAGATCCACACGGATCTACGCGAGTATGGCGAGCGCTGCGGGCCCAATCGAGTCCATCGCCTGATGC
GCCACGCGGGAATACGAGCGCAGGTTGGTTACCGTAAGCCACGCCATAGGGCCGGGGAGCTACACAAAGTGACACCGAATATCCTGCAGCGGCAGTTCAA
TCCGCAAGCGCCGAACGAGAGCTGGGTAACGGATATTACCTATATCCGCACACATGAAGGCTGGTTGTATCTGGCGGTTGTTCTGGATCTGTTCTCAAGA
CGGGTGATCGGTTGGTCAATGCAATCCCGGATAACCAAGGAGCTGGCGCTGGATGCGCTGCTGATGGCGGTCTGGCGCCGCAAGCCTGAAGGCAAGGTGG
TTGTTCACTCCGACCAAGGCAGTCAATACACCAGCCACGACTGGGATTCGTTTCTGAGGGCGCATGACCTGGAGGGGAGTATGAGTCGGCGCGGTAACTG
CCACGACAATGCTGTTGCTGAGAGCTTCTTCCAGCTGTTGAAGCGGGAAAGAATAAAGCGAAAGATTTACAGCAGCCGTGACGCCGCTCGGGCAGATATT
TTTGATTATATCGAGATGTTCTATAACAACCGGCGGCGCCATGGTTCTAATGAAAAGCTGTCACCGGTAGAGTATGAAAAGCGTCACCAAGAACGGCTGG
GAAGTGTCTAGAGAACTGGTGGCGATTCA
AGGCAGTTCGTCAGGTGACGGATCGTGGTCACAGTGTGGCCCAAGTGGCTGATCGTCTCGGTGTCACCACTCACAGTCTGTATGCCTGGATCAGGAAGTT
TGGCCCGGATTCCGAGCAGCATCAGGCCAATGCAGATGATCAAGTCGAGATTCGCCGACTCCAGAAAGAGCTCAAGCGCGTTACCGAAGAGCGAGACATC
CTAAAAAAAGCCGCGGCGTACTTCGCCAGTCAGTCCGATTGAGGTACGCCTTCATTCAGGAGCACAGCGAGCGGTGGGCAATTCGCTCACTGTGTTCCCT
GTTGGACGTTCACCCCAGTGGCTTTTATGCCTGGCAGAAACAGCCTCGTTCAGCTCGAGCAATCGAGGACGAGCGCCTGTCCGGGCTGATAAAGCAATTC
TGGTTGGAATCTGGAGCCGTGTATGGCTACAGGAAGATCCACACGGATCTACGCGAGTATGGCGAGCGCTGCGGGCCCAATCGAGTCCATCGCCTGATGC
GCCACGCGGGAATACGAGCGCAGGTTGGTTACCGTAAGCCACGCCATAGGGCCGGGGAGCTACACAAAGTGACACCGAATATCCTGCAGCGGCAGTTCAA
TCCGCAAGCGCCGAACGAGAGCTGGGTAACGGATATTACCTATATCCGCACACATGAAGGCTGGTTGTATCTGGCGGTTGTTCTGGATCTGTTCTCAAGA
CGGGTGATCGGTTGGTCAATGCAATCCCGGATAACCAAGGAGCTGGCGCTGGATGCGCTGCTGATGGCGGTCTGGCGCCGCAAGCCTGAAGGCAAGGTGG
TTGTTCACTCCGACCAAGGCAGTCAATACACCAGCCACGACTGGGATTCGTTTCTGAGGGCGCATGACCTGGAGGGGAGTATGAGTCGGCGCGGTAACTG
CCACGACAATGCTGTTGCTGAGAGCTTCTTCCAGCTGTTGAAGCGGGAAAGAATAAAGCGAAAGATTTACAGCAGCCGTGACGCCGCTCGGGCAGATATT
TTTGATTATATCGAGATGTTCTATAACAACCGGCGGCGCCATGGTTCTAATGAAAAGCTGTCACCGGTAGAGTATGAAAAGCGTCACCAAGAACGGCTGG
GAAGTGTCTAGAGAACTGGTGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 64 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MSSKRYPEEFKIEAVRQVTDRGHSVAQVADRLGVTTHSLYAWIRKFGPDSEQHQANADDQVEIRRLQKELKRVTEERDILKKAAAYFASQSD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 972 bp | 323 aa | 240 | 1211 | + | No |
Description : Second part of the transposase
ORF sequence :
SSRDSPTPERAQARYRRARHPKKSRGVLRQSVRLRYAFIQEHSERWAIRSLCSLLDVHPSGFYAWQKQPRSARAIEDERLSGLIKQFWLESGAVYGYRKI
HTDLREYGERCGPNRVHRLMRHAGIRAQVGYRKPRHRAGELHKVTPNILQRQFNPQAPNESWVTDITYIRTHEGWLYLAVVLDLFSRRVIGWSMQSRITK
ELALDALLMAVWRRKPEGKVVVHSDQGSQYTSHDWDSFLRAHDLEGSMSRRGNCHDNAVAESFFQLLKRERIKRKIYSSRDAARADIFDYIEMFYNNRRR
HGSNEKLSPVEYEKRHQERLGSV
HTDLREYGERCGPNRVHRLMRHAGIRAQVGYRKPRHRAGELHKVTPNILQRQFNPQAPNESWVTDITYIRTHEGWLYLAVVLDLFSRRVIGWSMQSRITK
ELALDALLMAVWRRKPEGKVVVHSDQGSQYTSHDWDSFLRAHDLEGSMSRRGNCHDNAVAESFFQLLKRERIKRKIYSSRDAARADIFDYIEMFYNNRRR
HGSNEKLSPVEYEKRHQERLGSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 64 | 1211 | + | Yes |
Chemistry : DDE
ORF sequence :
MSSKRYPEEFKIEAVRQVTDRGHSVAQVADRLGVTTHSLYAWIRKFGPDSEQHQANADDQVEIRRLQKELKRVTEERDILKKSRGVLRQSVRLRYAFIQE
HSERWAIRSLCSLLDVHPSGFYAWQKQPRSARAIEDERLSGLIKQFWLESGAVYGYRKIHTDLREYGERCGPNRVHRLMRHAGIRAQVGYRKPRHRAGEL
HKVTPNILQRQFNPQAPNESWVTDITYIRTHEGWLYLAVVLDLFSRRVIGWSMQSRITKELALDALLMAVWRRKPEGKVVVHSDQGSQYTSHDWDSFLRA
HDLEGSMSRRGNCHDNAVAESFFQLLKRERIKRKIYSSRDAARADIFDYIEMFYNNRRRHGSNEKLSPVEYEKRHQERLGSV
HSERWAIRSLCSLLDVHPSGFYAWQKQPRSARAIEDERLSGLIKQFWLESGAVYGYRKIHTDLREYGERCGPNRVHRLMRHAGIRAQVGYRKPRHRAGEL
HKVTPNILQRQFNPQAPNESWVTDITYIRTHEGWLYLAVVLDLFSRRVIGWSMQSRITKELALDALLMAVWRRKPEGKVVVHSDQGSQYTSHDWDSFLRA
HDLEGSMSRRGNCHDNAVAESFFQLLKRERIKRKIYSSRDAARADIFDYIEMFYNNRRRHGSNEKLSPVEYEKRHQERLGSV
Blast result :
Comments
ISAbo1 is 83 % (ORFA) aa similar to ISEhe3 and 80% (ORFB) aa similar to ISPca2. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Schneiker,S., Martins dos Santos,V.A., Bartels,D., Bekel,T., Brecht,M., Buhrmester,J., Chernikova,T.N., Denaro,R., Ferrer,M., Gertler,C., Goesmann,A., Golyshina,O.V., Kaminski,F., Khachane,A.N., Lang,S., Linke,B., McHardy,A.C., Meyer,F., Nechitaylo,T., Puhler,A., Regenhardt,D., Rupp,O., Sabirova,J.S., Selbitschka,W., Yakimov,M.M., Timmis,K.N., Vorholter,F.J., Weidner,S., Kaiser,O. and Golyshin,P.N. (2006) Nat. Biotechnol. 24 (8), 997-1004.