ISPin3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008709 | ND | Psychromonas ingrahamii | Psychromonas ingrahamii 37 |
DNA section
IS Length : 1215 bp
Ends
IR Length : 24/34
IRL : TATTCTGTTCTAATTGAACTGGATACTTTAATTTTGGATAATACAATCCA
IRR : TATTCTGGTCAAGTCCAACCGGACACCTGACTTTGTAAATTTTCATAGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAGTTAGGA | TTACCCGCTG | 0 |
DNA sequence
TATTCTGTTCTAATTGAACTGGATACTTTAATTTTGGATAATACAATCCACTATTGAGGTGTACATGAATACAAAACGTCAATACAAAACTTATACGAGT
GAATTTAAAACAGAAGCATTAGCGTTAATCAGTGAGCAAGATTATAGCGTTCAGGAGGCAGCTTCTGCTTTAGGTATCATAACTAGCCTGCTTTATAGCT
GGAAACAAAAAACCGAAGAGCATGCCAATTCAACGGTAAATTCTGATGAACGGACTGAGTTATTATCCTTACGAAAAGAAGTTAAACAGCTTCGTATGGA
AAAAGAAATACTAAAAAAGGCCAGCGCCTTCTTCGCAAAAGAAATGAAGTAAAGTTTCGATATATTCGAGCATATCGCTCTAAATATTCAATAAAACTAC
TTTGTAATGTACTACAAGTGAGTCGTAGTGCATTTTATGATTGGCTTGAACGGCCAGCTAAAATAATGAGTGAAGAAGAGCTGAATATGTATCGTACGGC
GCGTCAGCTATTTAAACGCAGTCGTGGCAGTTTAGGCTCTCGCCAGTTGTGCAAGAACTTGAGACGAGCGGGTTTTAATGTAGGCCGTTACTGTACGCGC
AGCATCATGCGTAAGCTGAAACTTGTGGTGACCCAACGCCAGGCATATAAAGTAACAACAAAGCGTAAGCATAGTGATAGCGTTGCTGATAACGTATTAA
ACCAGCAGTTCAATCCCACACAGGCAAACTTAGTTTGGGCGGGTGATGTAACATATTTGAGAACCAATCAAGGCTGGATGTATTTAGCCATTGTTATGGA
CTTATACTCCCGCAGGATTATAGGCTGGTCTATCTCGAAACGTATGACGGTTGATTTAGTTGAACGTGCATTACAAATGGCGATTAACATCAGGCAACCT
AAAGCTGAATTAATGTTCCATAGTGATAGGGGCTCTCAATATACTAGCGGGCTATTTAGTCGATTGAATAACGGCCTCAATGAGCAGTGTCGGTGCTTGT
TTAGATAATGCTGTTGTAGAACGATTTTTTGGTAGTTTAAAAAATGAATGGTTATTAAATGTTGTGCATTTAACACGTGAAAGGATGAAGATGGATGTTG
AAGAATATATCCGTTATTACAACCATGAACAACTTCATACAATACTCCGTAATTTAACAGCGATTAACTATGAAAATTTACAAAGTCAGGTGTCCGGTTG
GACTTGACCAGAATA
GAATTTAAAACAGAAGCATTAGCGTTAATCAGTGAGCAAGATTATAGCGTTCAGGAGGCAGCTTCTGCTTTAGGTATCATAACTAGCCTGCTTTATAGCT
GGAAACAAAAAACCGAAGAGCATGCCAATTCAACGGTAAATTCTGATGAACGGACTGAGTTATTATCCTTACGAAAAGAAGTTAAACAGCTTCGTATGGA
AAAAGAAATACTAAAAAAGGCCAGCGCCTTCTTCGCAAAAGAAATGAAGTAAAGTTTCGATATATTCGAGCATATCGCTCTAAATATTCAATAAAACTAC
TTTGTAATGTACTACAAGTGAGTCGTAGTGCATTTTATGATTGGCTTGAACGGCCAGCTAAAATAATGAGTGAAGAAGAGCTGAATATGTATCGTACGGC
GCGTCAGCTATTTAAACGCAGTCGTGGCAGTTTAGGCTCTCGCCAGTTGTGCAAGAACTTGAGACGAGCGGGTTTTAATGTAGGCCGTTACTGTACGCGC
AGCATCATGCGTAAGCTGAAACTTGTGGTGACCCAACGCCAGGCATATAAAGTAACAACAAAGCGTAAGCATAGTGATAGCGTTGCTGATAACGTATTAA
ACCAGCAGTTCAATCCCACACAGGCAAACTTAGTTTGGGCGGGTGATGTAACATATTTGAGAACCAATCAAGGCTGGATGTATTTAGCCATTGTTATGGA
CTTATACTCCCGCAGGATTATAGGCTGGTCTATCTCGAAACGTATGACGGTTGATTTAGTTGAACGTGCATTACAAATGGCGATTAACATCAGGCAACCT
AAAGCTGAATTAATGTTCCATAGTGATAGGGGCTCTCAATATACTAGCGGGCTATTTAGTCGATTGAATAACGGCCTCAATGAGCAGTGTCGGTGCTTGT
TTAGATAATGCTGTTGTAGAACGATTTTTTGGTAGTTTAAAAAATGAATGGTTATTAAATGTTGTGCATTTAACACGTGAAAGGATGAAGATGGATGTTG
AAGAATATATCCGTTATTACAACCATGAACAACTTCATACAATACTCCGTAATTTAACAGCGATTAACTATGAAAATTTACAAAGTCAGGTGTCCGGTTG
GACTTGACCAGAATA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 288 bp | 95 aa | 65 | 352 | + | No |
Description : First part of the transposase
ORF sequence :
MNTKRQYKTYTSEFKTEALALISEQDYSVQEAASALGIITSLLYSWKQKTEEHANSTVNSDERTELLSLRKEVKQLRMEKEILKKASAFFAKEMK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 723 bp | 240 aa | 286 | 1008 | + | No |
Description : Second part of the transposase
ORF sequence :
TASYGKRNTKKGQRLLRKRNEVKFRYIRAYRSKYSIKLLCNVLQVSRSAFYDWLERPAKIMSEEELNMYRTARQLFKRSRGSLGSRQLCKNLRRAGFNVG
RYCTRSIMRKLKLVVTQRQAYKVTTKRKHSDSVADNVLNQQFNPTQANLVWAGDVTYLRTNQGWMYLAIVMDLYSRRIIGWSISKRMTVDLVERALQMAI
NIRQPKAELMFHSDRGSQYTSGLFSRLNNGLNEQCRCLFR
RYCTRSIMRKLKLVVTQRQAYKVTTKRKHSDSVADNVLNQQFNPTQANLVWAGDVTYLRTNQGWMYLAIVMDLYSRRIIGWSISKRMTVDLVERALQMAI
NIRQPKAELMFHSDRGSQYTSGLFSRLNNGLNEQCRCLFR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 944 bp | 314 aa | 65 | 1008 | + | Yes |
Chemistry : DDE
ORF sequence :
MNTKRQYKTYTSEFKTEALALISEQDYSVQEAASALGIITSLLYSWKQKTEEHANSTVNSDERTELLSLRKEVKQLRMEKEILKKGQRLLRKRNEVKFRY
IRAYRSKYSIKLLCNVLQVSRSAFYDWLERPAKIMSEEELNMYRTARQLFKRSRGSLGSRQLCKNLRRAGFNVGRYCTRSIMRKLKLVVTQRQAYKVTTK
RKHSDSVADNVLNQQFNPTQANLVWAGDVTYLRTNQGWMYLAIVMDLYSRRIIGWSISKRMTVDLVERALQMAINIRQPKAELMFHSDRGSQYTSGLFSR
LNNGLNEQCRCLFR
IRAYRSKYSIKLLCNVLQVSRSAFYDWLERPAKIMSEEELNMYRTARQLFKRSRGSLGSRQLCKNLRRAGFNVGRYCTRSIMRKLKLVVTQRQAYKVTTK
RKHSDSVADNVLNQQFNPTQANLVWAGDVTYLRTNQGWMYLAIVMDLYSRRIIGWSISKRMTVDLVERALQMAINIRQPKAELMFHSDRGSQYTSGLFSR
LNNGLNEQCRCLFR
Blast result :
Comments
ISPin3 is 72% (ORFA) and 78% (ORFB) aa similar to ISSod1. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Thompson,L.S., Brettin,T., Bruce,D., Han,C., Tapia,R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Staley,J. and Richardson,P.(2007) Direct submission.GenBank.