ISAfl1

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011567 ND Anoxybacillus flavithermus
Anoxybacillus flavithermus WK1
DNA section
IS Length : 1363 bp

Ends


IR Length : 23/31

IRL : CTGTAGATGAGCAAATTCGCTAACATAAATTTACAACCAAACAAAAGAAG
IRR : CTGTAGTTGAGCAATTTTAATCACAATAATTACCATATAAAAAGAGACAA

Insertion site


Left flankDirect repeatRight flankDR Length
ATTTCCTTTATTACTTATTTAAATTTT7
CCATTTTTTATATGAAGATAAGCACG6
TATCATTAGCTTCATTATTCATCATCA7

DNA sequence

CTGTAGATGAGCAAATTCGCTAACATAAATTTACAACCAAACAAAAGAAGAGACATGAACCCGATTCCTTGATTAGAATAGATGTGTCACCAAACCATTC
ACAAGGAGGTTCATGTCTCATGAATAGATTAGCACATCATCAAGGAATCGACAAGTTTTTCACGATGTTAGGGTTGGCACTTTATTTCTCGAAACCTGTG
ATGAAGCATCTCGTTCATATCGTGGATGCGATGGTGACGAAAGGTTTTTCCGGGACGTTGACCGATCTTCATCATGGTAGTTTTCACCCGAATCATCGCA
CGACACTGAGCCATTTTTTCACGAAAAGTCCGTGGGATGAAGAAACATTGCTTCACAAACTCCAGCAGTGGATTCTTCGTCGTGTCGAACGCAGCCCGAA
ACGAGAGAATTCTCCCCTGTTTGTTTCAATCGATGATACAATTTGCCAAAAAACGAAGCCTTCGTCACGGGCAACACACGCCATTCAAGGGTGTGATTGG
CACTATTCTCATGCCGAGAAAAAGTCAATCTGGGGGCATTCTCTCGTTTGGCTCATGGTTCATACGATGACCCAAGCGTTCCCTTTTGCCTTTCGCCTCT
ATGACAAGACGGCAGGGAAAAGCAAAGGGGAACTTGCGATGGAGATGCTTTCTTCTTTGGATGTGAGTCGCCCCGTTTATGTGCTCATGGATTCGTGGTA
TCCATCGCAAACCCTCGTGGAAGCTTGTCTGAAAAAAGGATTCCACGTGATTGCGATGCTCAAGACGAACCGAATTCTCTACCCGAAAGGTATCGCCATC
CAAGCGAAGCAGTTTGCCCACTACATCGAACCGAAAGACACTCACCTCGTCACGGTGGGAAAAGAGCAGTATCGGGTGTATCGCTACGAAGGTGCTCTGA
ACGGTCTCAAGGATGCAGTGGTGATCCTCGCTTGGAAAGCGGATCAACCGATGACATCGGAACATCTTCATTGCGTCTTGAGCACGGATCGCAAGTTGAG
CGATGAAGACATCTTGCGCTACTATGCTGAGCGTTGGTCGATCGAATGTTTTTTCCGTCAAGCGAAAGACCAGCTGAAACTCGATGGATACCGCGTTCGC
GGGCGTCGGGCAGTGAAACGGTATTGGATCTTGGTGCAACTTGCGTATGTGTACAGCATGTTCGAGTCGAACAGTGATTTTTCGGATGGGCTCGATCTCC
TGCGCAAGAGAAAAGGACATAGCCTCGTGGAGTTCATTTACTGTGCAGCAAAACAAAATATTCCCATCGATACCGTAAAAAAACAGCTCCACGTGGCATA
AGGGGTATCCTGTTTGTCTCTTTTTATATGGTAATTATTGTGATTAAAATTGCTCAACTACAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1233 bp410 aa691301+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIRIDVSPNHSQGGSCLMNRLAHHQGIDKFFTMLGLALYFSKPVMKHLVHIVDAMVTKGFSGTLTDLHHGSFHPNHRTTLSHFFTKSPWDEETLLHKLQQ
WILRRVERSPKRENSPLFVSIDDTICQKTKPSSRATHAIQGCDWHYSHAEKKSIWGHSLVWLMVHTMTQAFPFAFRLYDKTAGKSKGELAMEMLSSLDVS
RPVYVLMDSWYPSQTLVEACLKKGFHVIAMLKTNRILYPKGIAIQAKQFAHYIEPKDTHLVTVGKEQYRVYRYEGALNGLKDAVVILAWKADQPMTSEHL
HCVLSTDRKLSDEDILRYYAERWSIECFFRQAKDQLKLDGYRVRGRRAVKRYWILVQLAYVYSMFESNSDFSDGLDLLRKRKGHSLVEFIYCAAKQNIPI
DTVKKQLHVA

 

Blast result :
Comments
ISAfl1 is 51% aa similar to ISCth16.
References
1] ISfinder submission (2012)
2] Saw,J.H., Mountain,B.W., Feng,L., Omelchenko,M.V., Hou,S., Saito,J.A., Stott,M.B., Li,D., Zhao,G., Wu,J., Galperin,M.Y., Koonin,E.V., Makarova,K.S., Wolf,Y.I., Rigden,D.J., Dunfield,P.F., Wang,L. and Alam,M. (2008) Genome Biol. 9 (11), R161