ISEam1
- Family IS3
- Group IS51
Isoform Synonym(s) ISEch10
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF264951 | ND | Erwinia amylovora | Erwinia amylovora 2-95 Dickeya dadantii 3937 |
DNA section
IS Length : 1230 bp
Ends
IR Length : 29/36
IRL : TGGACTGCCCCTGCCACGGTAGACAATNCTGCCCTATATTTTGAGCATAG
IRR : TGGACTGACCCCACAACCGTAGACAAATCCTGTCCTCACGACGAGGCCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAATAAGTA | ATTTTGAGCA | 0 |
DNA sequence
TGGACTGCCCCTGCCACGGTAGACAATNCTGCCCTATATTTTGAGCATAGGAGGAGCGTATGGGCACACCACGATTTACACCTGAATTTAAAGAAGAGGC
GGTCCGTCAGATAACGGAACGTGGCTATTCCGTTGCTGACGTTTCCGACCGGCTGGGCGTCTCCGCGCACAGCCTTTACAAGTGGCTACGCGCCATAAAA
CCCGATAATAGCGAGCAGCATGCCCGGGATTTACTGGAGGCCAAAAGCGAGATCCTGAAACTACGGGCGCAGCTAAAACGTACCGAAGAAGAACGGGATA
TCCTGAAAAAGGCCGCGCGGTACTTTGCAAGGGAGCCCGACTGAAGTACCGCTTTATTAATGAGCACCGCGCTGTATGGCGTGTCATGACGATGTGTCGG
GTACTGAATGTCGNCCGGGCCGGGTTCTATGCGTGGCTGCATAACCCGGTCTCCGCGCGTGATAAAGATAACCAGCGTCTGCTGACGCTTATCCGTGACT
CCTGTTCACTGAGCGGAGGCGTATACGGTTACCGGCGGGTTCATGGCGACCTGAACGAAATCGGAGAAACCTGCGGTAAAAACCGGGTGGGCCGCATCAT
GCAGCTGAACCGAATTAAAGCTGTGCGCGGCTATAAAGCCCCACGTCGTATCGCCGGCAGACCTTCAGTGGTAGCGCCGAATCGCGTGCAGCGGCAGTTT
ACCGTCGANCGGGCCAATCAGGTCTGGGTCACCGATATTACTTATATCCGCACCTGGCAGGGCTGGCTGTATCTGGCGGTGGTTATCGATCTCTTCGCCC
GTAACGTANTGGGCTGGTCGATGAAACCCACTCTCTCACGCGAACTGGCGCTGGATGCGCTGATGATGGCGGTCTGGCGACGTAAACCTGACGGCGAGGT
CATCGTGCACTCAGACCAGGGCAGCCAGTACGGCAGCGACGACTGGCAGCGGTTCTGTCGGGCCAATAACCTGTCCCCGAGTATGAGCCGGCGTGGCAAC
TGCTGGGATAATGCGGTGGCCGAATCGTTCTTCAGTTCGCTGAAAAAAGAACGCATCAGGAAGAGAATATACAAAACCCGGGATCTGGCCCGGGCGGATA
TCTTCGATTACATTGAAGTGTTCTACAACCGGGCCCGGCGCCACAGCCATCTCGGCGGCGTCAGTCCGGAGGCCTTCGAACAGGCCTCGTCGTGAGGACA
GGATTTGTCTACGGTTGTGGGGTCAGTCCA
GGTCCGTCAGATAACGGAACGTGGCTATTCCGTTGCTGACGTTTCCGACCGGCTGGGCGTCTCCGCGCACAGCCTTTACAAGTGGCTACGCGCCATAAAA
CCCGATAATAGCGAGCAGCATGCCCGGGATTTACTGGAGGCCAAAAGCGAGATCCTGAAACTACGGGCGCAGCTAAAACGTACCGAAGAAGAACGGGATA
TCCTGAAAAAGGCCGCGCGGTACTTTGCAAGGGAGCCCGACTGAAGTACCGCTTTATTAATGAGCACCGCGCTGTATGGCGTGTCATGACGATGTGTCGG
GTACTGAATGTCGNCCGGGCCGGGTTCTATGCGTGGCTGCATAACCCGGTCTCCGCGCGTGATAAAGATAACCAGCGTCTGCTGACGCTTATCCGTGACT
CCTGTTCACTGAGCGGAGGCGTATACGGTTACCGGCGGGTTCATGGCGACCTGAACGAAATCGGAGAAACCTGCGGTAAAAACCGGGTGGGCCGCATCAT
GCAGCTGAACCGAATTAAAGCTGTGCGCGGCTATAAAGCCCCACGTCGTATCGCCGGCAGACCTTCAGTGGTAGCGCCGAATCGCGTGCAGCGGCAGTTT
ACCGTCGANCGGGCCAATCAGGTCTGGGTCACCGATATTACTTATATCCGCACCTGGCAGGGCTGGCTGTATCTGGCGGTGGTTATCGATCTCTTCGCCC
GTAACGTANTGGGCTGGTCGATGAAACCCACTCTCTCACGCGAACTGGCGCTGGATGCGCTGATGATGGCGGTCTGGCGACGTAAACCTGACGGCGAGGT
CATCGTGCACTCAGACCAGGGCAGCCAGTACGGCAGCGACGACTGGCAGCGGTTCTGTCGGGCCAATAACCTGTCCCCGAGTATGAGCCGGCGTGGCAAC
TGCTGGGATAATGCGGTGGCCGAATCGTTCTTCAGTTCGCTGAAAAAAGAACGCATCAGGAAGAGAATATACAAAACCCGGGATCTGGCCCGGGCGGATA
TCTTCGATTACATTGAAGTGTTCTACAACCGGGCCCGGCGCCACAGCCATCTCGGCGGCGTCAGTCCGGAGGCCTTCGAACAGGCCTCGTCGTGAGGACA
GGATTTGTCTACGGTTGTGGGGTCAGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 60 | 344 | + | No |
Description : First part of the transposase
ORF sequence :
MGTPRFTPEFKEEAVRQITERGYSVADVSDRLGVSAHSLYKWLRAIKPDNSEQHARDLLEAKSEILKLRAQLKRTEEERDILKKAARYFAREPD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 984 bp | 327 aa | 212 | 1195 | + | No |
Description : Second part of the transposase
ORF sequence :
RAACPGFTGGQKRDPETTGAAKTYRRRTGYPEKGRAVLCKGARLKYRFINEHRAVWRVMTMCRVLNVXRAGFYAWLHNPVSARDKDNQRLLTLIRDSCSL
SGGVYGYRRVHGDLNEIGETCGKNRVGRIMQLNRIKAVRGYKAPRRIAGRPSVVAPNRVQRQFTVXRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVX
GWSMKPTLSRELALDALMMAVWRRKPDGEVIVHSDQGSQYGSDDWQRFCRANNLSPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDY
IEVFYNRARRHSHLGGVSPEAFEQASS
SGGVYGYRRVHGDLNEIGETCGKNRVGRIMQLNRIKAVRGYKAPRRIAGRPSVVAPNRVQRQFTVXRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVX
GWSMKPTLSRELALDALMMAVWRRKPDGEVIVHSDQGSQYGSDDWQRFCRANNLSPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDY
IEVFYNRARRHSHLGGVSPEAFEQASS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1136 bp | 378 aa | 60 | 1195 | + | Yes |
Chemistry : DDE
ORF sequence :
MGTPRFTPEFKEEAVRQITERGYSVADVSDRLGVSAHSLYKWLRAIKPDNSEQHARDLLEAKSEILKLRAQLKRTEEERDILKKGRAVLCKGARLKYRFI
NEHRAVWRVMTMCRVLNVXRAGFYAWLHNPVSARDKDNQRLLTLIRDSCSLSGGVYGYRRVHGDLNEIGETCGKNRVGRIMQLNRIKAVRGYKAPRRIAG
RPSVVAPNRVQRQFTVXRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVXGWSMKPTLSRELALDALMMAVWRRKPDGEVIVHSDQGSQYGSDDWQRFC
RANNLSPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDYIEVFYNRARRHSHLGGVSPEAFEQASS
NEHRAVWRVMTMCRVLNVXRAGFYAWLHNPVSARDKDNQRLLTLIRDSCSLSGGVYGYRRVHGDLNEIGETCGKNRVGRIMQLNRIKAVRGYKAPRRIAG
RPSVVAPNRVQRQFTVXRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVXGWSMKPTLSRELALDALMMAVWRRKPDGEVIVHSDQGSQYGSDDWQRFC
RANNLSPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDYIEVFYNRARRHSHLGGVSPEAFEQASS
Blast result :
Comments
ISEam1 is 72 % (ORFA) aa similar to ISBcen21 and 73% (ORFB) aa similar to ISPca2. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] McGhee,G.C. and Jones,A.L. (2000) Appl. Environ. Microbiol. 66 (11), 4897-4907