ISEc15
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004998 | ND | Escherichia coli | Escherichia coli |
DNA section
IS Length : 1230 bp
Ends
IR Length : 29/36
IRL : TGGAGTGACCCCGAGCCCGTAGACAAATCTGCCCTATAGTTTGAGCATAG
IRR : TGGAGTGACCACGCTCCGGTAGACAGTTTCTGTCCTCACTGCGAGGCCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATCACTTAA | CATTTAAAGA | 0 |
DNA sequence
TGGAGTGACCCCGAGCCCGTAGACAAATCTGCCCTATAGTTTGAGCATAGGAGGAGTCTATGGGCACATCACGTTTTACCCCTGAATTTAAGGAAGAGGC
CGTCCGCCAGATCACCGAGCGGGGCTATTCTATTGCTGAAGTATCTGAACGGCTCGGCGTGTCGGCGCACAGCCTATATAAATGGCTCCGGGCTGTTAAG
CCTGACAACAGTGGACAGCAGGCACAGGATTTACTGGATGCCAGAACAGAAATTCTCAGGCTGAAAGCACAGCTTAAACGCACTGAGGAAGAGCGGGATA
TTCTGAAAAAGGCAGCGCGGTACTTTGCAAGGGAGCCCGACTGAAGTATCGCTTTATCAACGATCACCGTGAAATCTGGTCGATCGTTACAATGTGTCGG
GTCCTTAAGGTTGCCCGTGCCGGATTTTATGTCTGGCTCCATAACCCTGTATCTGCCGGAGAAAAGGATAACCAGCGACTGCTGGAACTCATCCGCGACT
CCTATACGCTGAGTGGTGGAGTTTACGGTTATCGCAGGGTTCATGGCGATCTTCGCGAAATTGGCGAAGTGTGCAGCAGAAACCGGGTTGCCAAAATCAT
GAGGAAAAACAGGATTCAGGCTATACATGGTTATAAAGTGCCACGCGGGAGCCGGGGACGATCGTCACTGATAGCGCCTAATCACGTGCGGCGTGAATTT
ACTGTAGTGAAGCCCAATCAGGTGTGGGTCACCGACATCACCTACATCCGCACCTGGCAGGGCTGGCTGTACCTGGCAGTGGTTATCGATCTTTTTGCCC
GTAATGTGGTGGGCTGGTCGATGAAGCCGACACTGTCGCGCGAGCTGGCACTGGATGCGCTGCTGATGGCGGTCTGGCGACGTAAACCTGAAGAAAACGT
CATTGTGCACAGCGATCAGGGCAGTCAGTATGGCAGTGATGACTGGCAGCGGTTCTGCCGGGCCAATAATCTGGTACCGAGCATGAGCCGTCGCGGCAAC
TGCTGGGATAATGCAGTGGCAGAATCGTTCTTCAGTTCACTCAAAAAAGAGCGCATCAGGAAGCGGATATACAAAACCCGGGACATGGCCCGGGCGGATA
TTTTCGATTATATTGAAGTGTTCTACAACCGGGCCCGGCGCCACAGTCATCTCGGCGGCGTCAGCCCGGAGGCCTTCGAAAAGGCCTCGCAGTGAGGACA
GAAACTGTCTACCGGAGCGTGGTCACTCCA
CGTCCGCCAGATCACCGAGCGGGGCTATTCTATTGCTGAAGTATCTGAACGGCTCGGCGTGTCGGCGCACAGCCTATATAAATGGCTCCGGGCTGTTAAG
CCTGACAACAGTGGACAGCAGGCACAGGATTTACTGGATGCCAGAACAGAAATTCTCAGGCTGAAAGCACAGCTTAAACGCACTGAGGAAGAGCGGGATA
TTCTGAAAAAGGCAGCGCGGTACTTTGCAAGGGAGCCCGACTGAAGTATCGCTTTATCAACGATCACCGTGAAATCTGGTCGATCGTTACAATGTGTCGG
GTCCTTAAGGTTGCCCGTGCCGGATTTTATGTCTGGCTCCATAACCCTGTATCTGCCGGAGAAAAGGATAACCAGCGACTGCTGGAACTCATCCGCGACT
CCTATACGCTGAGTGGTGGAGTTTACGGTTATCGCAGGGTTCATGGCGATCTTCGCGAAATTGGCGAAGTGTGCAGCAGAAACCGGGTTGCCAAAATCAT
GAGGAAAAACAGGATTCAGGCTATACATGGTTATAAAGTGCCACGCGGGAGCCGGGGACGATCGTCACTGATAGCGCCTAATCACGTGCGGCGTGAATTT
ACTGTAGTGAAGCCCAATCAGGTGTGGGTCACCGACATCACCTACATCCGCACCTGGCAGGGCTGGCTGTACCTGGCAGTGGTTATCGATCTTTTTGCCC
GTAATGTGGTGGGCTGGTCGATGAAGCCGACACTGTCGCGCGAGCTGGCACTGGATGCGCTGCTGATGGCGGTCTGGCGACGTAAACCTGAAGAAAACGT
CATTGTGCACAGCGATCAGGGCAGTCAGTATGGCAGTGATGACTGGCAGCGGTTCTGCCGGGCCAATAATCTGGTACCGAGCATGAGCCGTCGCGGCAAC
TGCTGGGATAATGCAGTGGCAGAATCGTTCTTCAGTTCACTCAAAAAAGAGCGCATCAGGAAGCGGATATACAAAACCCGGGACATGGCCCGGGCGGATA
TTTTCGATTATATTGAAGTGTTCTACAACCGGGCCCGGCGCCACAGTCATCTCGGCGGCGTCAGCCCGGAGGCCTTCGAAAAGGCCTCGCAGTGAGGACA
GAAACTGTCTACCGGAGCGTGGTCACTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 60 | 344 | + | No |
Description : First part of the transposase
ORF sequence :
MGTSRFTPEFKEEAVRQITERGYSIAEVSERLGVSAHSLYKWLRAVKPDNSGQQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1109 bp | 302 aa | 87 | 1195 | + | No |
Description : Second part of the transposase
ORF sequence :
GRAGYSEKGSAVLCKGARLKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHNPVSAGEKDNQRLLELIRDSYTLSGGVYGYRRVHGDLREIGEVCSRNR
VAKIMRKNRIQAIHGYKVPRGSRGRSSLIAPNHVRREFTVVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRELALDALLMAVWRRK
PEENVIVHSDQGSQYGSDDWQRFCRANNLVPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRARRHSHLGGVSPEAFEKA
SQ
VAKIMRKNRIQAIHGYKVPRGSRGRSSLIAPNHVRREFTVVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRELALDALLMAVWRRK
PEENVIVHSDQGSQYGSDDWQRFCRANNLVPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRARRHSHLGGVSPEAFEKA
SQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1136 bp | 378 aa | 60 | 1195 | + | Yes |
Chemistry : DDE
ORF sequence :
MGTSRFTPEFKEEAVRQITERGYSIAEVSERLGVSAHSLYKWLRAVKPDNSGQQAQDLLDARTEILRLKAQLKRTEEERDILKKGSAVLCKGARLKYRFI
NDHREIWSIVTMCRVLKVARAGFYVWLHNPVSAGEKDNQRLLELIRDSYTLSGGVYGYRRVHGDLREIGEVCSRNRVAKIMRKNRIQAIHGYKVPRGSRG
RSSLIAPNHVRREFTVVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRELALDALLMAVWRRKPEENVIVHSDQGSQYGSDDWQRFC
RANNLVPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRARRHSHLGGVSPEAFEKASQ
NDHREIWSIVTMCRVLKVARAGFYVWLHNPVSAGEKDNQRLLELIRDSYTLSGGVYGYRRVHGDLREIGEVCSRNRVAKIMRKNRIQAIHGYKVPRGSRG
RSSLIAPNHVRREFTVVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRELALDALLMAVWRRKPEENVIVHSDQGSQYGSDDWQRFC
RANNLVPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRARRHSHLGGVSPEAFEKASQ
Blast result :
Comments
ISEc15 is 71% (ORFA) aa similar to IS ISXac3 and 75% (ORFB) aa similar to ISPca2. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Zienkiewicz,M., Kern-Zdanowicz,I., Golebiewski,M. and Ceglowski,P.(2003) Direct submission GenBank.