ISEc16
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAJU01000005.1 | ND | Escherichia coli | Escherichia coli F11 |
DNA section
IS Length : 1244 bp
Ends
IR Length : 33/41
IRL : TGATCTTACCCACCAATAGTGGACACAGGACTAAGTGAGTAAACTCTCAA
IRR : TGATCCTACCCACGTAATGTGGACACGGCCCTAAGCGAGATTATGGTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTAAGATATC | TATTACCTGT | 0 |
DNA sequence
TGATCTTACCCACCAATAGTGGACACAGGACTAAGTGAGTAAACTCTCAACCAGAGGTGACTCATGACAAAACCAGTATCAATCAGCAAGAAGCCCCGTA
AACAACATACGCCTGAATTTCGTAACGAAGCCCTGAAACTCGCTGAACGCATCGGTGTGGCCGCCGCAGCCCGTGAACTCAGCCTGTATGAATCTCAGCT
TTATGCCTGGTGCAGTAAACAGCAGCAACAAATGAGTTCGTCAGAGCGCGAAAGCGAACTGGCCGCTGAAAATGTCCGCCTTAAACGACAACTGGCGGAG
CAGGCTGAGGAACTGGCCATCCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTCATCGAAAATCATCGGGCAGAGTTCAG
CATCAAAGCGATGTGTCGTGTACTTCGGGTTGCCCGCAGCGGCTGGTATGTCTGGCTCAGGCGTCGTCACCAGATGAGCCTGCGCCAACAGTTTCGGCTC
ACCTGCGATGCCGCTGTCCATAAGGCATTCTTTGAGGCAAAGCAGCGATACGGTGCTCCCCGCCTTGCTGACGAAATGCCGGAGTTCAATATTAAAACCA
TTGCTGCCAGCCTGCGTCGTCAGGGGCTGCGGGCGAAAGCCAGCCGGAAGTTCAGCCCGGTCAGCTACCGTGCACATGGCCTGCCCGTATTGGAGAATCT
GCTGGAGCAGGACTTCAGCGCCAGCGGCCCGAACCAGAAGTGGGCGGGTGACATCACGTACTTGCGTACCGATGAGGGCTGGTTGTATCTCGCAGTAGTC
ATCGACCTGTGGTCACGCGCCGTCATTGGCTGGTCGATGTCACCGCGAATGACAGCACAACTGGCCTGTGATGCACTGCAAATGGCGTTGTGGCGGAGAA
GACGCCCGGAAAGCGTCATTGTTCATACGGACCGTGGTGGTCAATACTGTTCAGGGGATTATCAGGCGCTGCTGAAGCGACACAACCTGCGTGGCAGTAT
GAGTGCGAAAGGTTACTGTTATGACAATGCCTGTGTGGAAAGCTTCTTTCATTCGCTGAAGGTGGAATGTATCCACGGGGAACGCTTTAGCAGCCGGGAA
ATAATGCGGGCAACGGTGTTTAATTATATCGAGTGTGATTACAATCGCTGGCGTCGTCACAGTGCCTGTGGCGGTCTCAGCCCGGAACAATTTGAAAACC
ATAATCTCGCTTAGGGCCGTGTCCACATTACGTGGGTAGGATCA
AACAACATACGCCTGAATTTCGTAACGAAGCCCTGAAACTCGCTGAACGCATCGGTGTGGCCGCCGCAGCCCGTGAACTCAGCCTGTATGAATCTCAGCT
TTATGCCTGGTGCAGTAAACAGCAGCAACAAATGAGTTCGTCAGAGCGCGAAAGCGAACTGGCCGCTGAAAATGTCCGCCTTAAACGACAACTGGCGGAG
CAGGCTGAGGAACTGGCCATCCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTCATCGAAAATCATCGGGCAGAGTTCAG
CATCAAAGCGATGTGTCGTGTACTTCGGGTTGCCCGCAGCGGCTGGTATGTCTGGCTCAGGCGTCGTCACCAGATGAGCCTGCGCCAACAGTTTCGGCTC
ACCTGCGATGCCGCTGTCCATAAGGCATTCTTTGAGGCAAAGCAGCGATACGGTGCTCCCCGCCTTGCTGACGAAATGCCGGAGTTCAATATTAAAACCA
TTGCTGCCAGCCTGCGTCGTCAGGGGCTGCGGGCGAAAGCCAGCCGGAAGTTCAGCCCGGTCAGCTACCGTGCACATGGCCTGCCCGTATTGGAGAATCT
GCTGGAGCAGGACTTCAGCGCCAGCGGCCCGAACCAGAAGTGGGCGGGTGACATCACGTACTTGCGTACCGATGAGGGCTGGTTGTATCTCGCAGTAGTC
ATCGACCTGTGGTCACGCGCCGTCATTGGCTGGTCGATGTCACCGCGAATGACAGCACAACTGGCCTGTGATGCACTGCAAATGGCGTTGTGGCGGAGAA
GACGCCCGGAAAGCGTCATTGTTCATACGGACCGTGGTGGTCAATACTGTTCAGGGGATTATCAGGCGCTGCTGAAGCGACACAACCTGCGTGGCAGTAT
GAGTGCGAAAGGTTACTGTTATGACAATGCCTGTGTGGAAAGCTTCTTTCATTCGCTGAAGGTGGAATGTATCCACGGGGAACGCTTTAGCAGCCGGGAA
ATAATGCGGGCAACGGTGTTTAATTATATCGAGTGTGATTACAATCGCTGGCGTCGTCACAGTGCCTGTGGCGGTCTCAGCCCGGAACAATTTGAAAACC
ATAATCTCGCTTAGGGCCGTGTCCACATTACGTGGGTAGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 64 | 363 | + | No |
Description : First part of the transposase
ORF sequence :
MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWCSKQQQQMSSSERESELAAENVRLKRQLAEQAEELAILQKAATYFAKRLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 309 | 1214 | + | No |
Description : Second part of the transposase
ORF sequence :
GTGHPPKGRDILREAPEMKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADEMPEFNIKTIAA
SLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRRRP
ESVIVHTDRGGQYCSGDYQALLKRHNLRGSMSAKGYCYDNACVESFFHSLKVECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPEQFENHNL
A
SLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRRRP
ESVIVHTDRGGQYCSGDYQALLKRHNLRGSMSAKGYCYDNACVESFFHSLKVECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPEQFENHNL
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 64 | 1214 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWCSKQQQQMSSSERESELAAENVRLKRQLAEQAEELAILQKGRDILREAPEM
KYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADEMPEFNIKTIAASLRRQGLRAKASRKFSPV
SYRAHGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRRRPESVIVHTDRGGQYCSGDY
QALLKRHNLRGSMSAKGYCYDNACVESFFHSLKVECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPEQFENHNLA
KYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADEMPEFNIKTIAASLRRQGLRAKASRKFSPV
SYRAHGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRRRPESVIVHTDRGGQYCSGDY
QALLKRHNLRGSMSAKGYCYDNACVESFFHSLKVECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPEQFENHNLA
Blast result :
Comments
Genome in progress.
ISEc16 is 89% (ORFA) aa similar to IS1230B and 90% (ORFB) aa similar to IS3. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
ISEc16 is 89% (ORFA) aa similar to IS1230B and 90% (ORFB) aa similar to IS3. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] NCBI Microbial Genomes Annotation Project. (2005) Direct submission GenBank.