ISEc14
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAWW01000001 | ND | Escherichia coli | Escherichia coli B |
DNA section
IS Length : 1271 bp
Ends
IR Length : 30/36
IRL : TGGACTGACCCCGCCCCGGTAGACAATCCTGCCCTATAGTTTGGACTGAC
IRR : TGGACTGACCCCATGCCCGTAGACAAATTCTGTCCTCACGATGAGGCCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGGTCATCG | TACTTTCCAC | 0 |
DNA sequence
TGGACTGACCCCGCCCCGGTAGACAATCCTGCCCTATAGTTTGGACTGACCCCGCCCCGGTAGACAATCCTGCCCTATAGTTTGAGCATAGGAGGAGCGT
ATGGGCATACCACGATTTACACCTGAATTTAAGGAAGAAGCCGTCCGTCAGATAACGGAACGCGGTTATTCCGTCGCTGAAGTATCTGACCGTCTGGGCG
TCTCTGCACACAGCCTCTACAAGTGGCTACGGGCTATCAAACCTGATAACAGCGAGCAGCATGCACGGGATTTACTGGAAGCTAAAAGCGAGATCCTGAA
ACTCCGGGCGCAGCTAAAACGCACCGAAGAAGAACGGGATATCCTGAAAAAGGCCGCGCGGTACTTTGCAAGGGAGCCCGACTGAAGTACCGCTTTATCA
ATGAGCACCGCACTGTATGGGGTGTGATGACGATGTGTCGGGTACTGAATGTCGCCCGGGCCGGGTTCTATGCGTGGCTGCATAACCCTGTCTCGGCGCG
TGATAAAGATAACCAGCGCCTGCTGATGCTTATCCGCGACTCATATTCCCTGAGCGGAGGCGTATACGGTTATCGACGGGTTCATGGCGATCTGAACGAA
ATCGGGGAAACCTGCGGTAAAAACCGGGTGGGTCGGATCATGCAACTGAACCGGATTAAAGCTGTGCGCGGCTATAAAGCTCCACGTCGTATCGCTGGCA
GGCCTTCAGTCGTTGCTCCTAATCGCGTGCAGCGGCAGTTCACCGTTGTCCGGGCCAACCAGGTCTGGGTCACTGATATTACTTATATCCGCACCTGGCA
GGGCTGGCTGTATCTGGCGGTGGTTATCGATCTCTTCGCCCGTAATGTGGTCGGCTGGTCGATGAAACCCACTCTCTCACGCGAACTGGCGCTCGACGCG
CTGATGATGGCGGTCTGGCGCCGAAAACCAGACGGCGAGGTCATCGTACATAGCGATCAAGGTAGTCAGTACGGCAGTGACGACTGGCAGCGCTTCTGCC
GGGCCAATAACCTGGCACCGAGCATGAGCCGGCGTGGCAACTGCTGGGATAATGCGGTGGCCGAATCGTTCTTCAGTTCACTGAAAAAAGAACGCATCAG
GAAGAGAATATACAAAACCCGGGATCTGGCCCGGGCGGATATCTTCGATTACATTGAAGTGTTCTACAACCGGGCCCGGCGCCACAGTCACCTCGGTGGC
GTCAGTCCGGAGGCCTTCGAACAGGCCTCATCGTGAGGACAGAATTTGTCTACGGGCATGGGGTCAGTCCA
ATGGGCATACCACGATTTACACCTGAATTTAAGGAAGAAGCCGTCCGTCAGATAACGGAACGCGGTTATTCCGTCGCTGAAGTATCTGACCGTCTGGGCG
TCTCTGCACACAGCCTCTACAAGTGGCTACGGGCTATCAAACCTGATAACAGCGAGCAGCATGCACGGGATTTACTGGAAGCTAAAAGCGAGATCCTGAA
ACTCCGGGCGCAGCTAAAACGCACCGAAGAAGAACGGGATATCCTGAAAAAGGCCGCGCGGTACTTTGCAAGGGAGCCCGACTGAAGTACCGCTTTATCA
ATGAGCACCGCACTGTATGGGGTGTGATGACGATGTGTCGGGTACTGAATGTCGCCCGGGCCGGGTTCTATGCGTGGCTGCATAACCCTGTCTCGGCGCG
TGATAAAGATAACCAGCGCCTGCTGATGCTTATCCGCGACTCATATTCCCTGAGCGGAGGCGTATACGGTTATCGACGGGTTCATGGCGATCTGAACGAA
ATCGGGGAAACCTGCGGTAAAAACCGGGTGGGTCGGATCATGCAACTGAACCGGATTAAAGCTGTGCGCGGCTATAAAGCTCCACGTCGTATCGCTGGCA
GGCCTTCAGTCGTTGCTCCTAATCGCGTGCAGCGGCAGTTCACCGTTGTCCGGGCCAACCAGGTCTGGGTCACTGATATTACTTATATCCGCACCTGGCA
GGGCTGGCTGTATCTGGCGGTGGTTATCGATCTCTTCGCCCGTAATGTGGTCGGCTGGTCGATGAAACCCACTCTCTCACGCGAACTGGCGCTCGACGCG
CTGATGATGGCGGTCTGGCGCCGAAAACCAGACGGCGAGGTCATCGTACATAGCGATCAAGGTAGTCAGTACGGCAGTGACGACTGGCAGCGCTTCTGCC
GGGCCAATAACCTGGCACCGAGCATGAGCCGGCGTGGCAACTGCTGGGATAATGCGGTGGCCGAATCGTTCTTCAGTTCACTGAAAAAAGAACGCATCAG
GAAGAGAATATACAAAACCCGGGATCTGGCCCGGGCGGATATCTTCGATTACATTGAAGTGTTCTACAACCGGGCCCGGCGCCACAGTCACCTCGGTGGC
GTCAGTCCGGAGGCCTTCGAACAGGCCTCATCGTGAGGACAGAATTTGTCTACGGGCATGGGGTCAGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 101 | 385 | + | No |
Description : First part of the transposase
ORF sequence :
MGIPRFTPEFKEEAVRQITERGYSVAEVSDRLGVSAHSLYKWLRAIKPDNSEQHARDLLEAKSEILKLRAQLKRTEEERDILKKAARYFAREPD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 286 | 1236 | + | No |
Description : Second part of the transposase
ORF sequence :
KRDPETPGAAKTHRRRTGYPEKGRAVLCKGARLKYRFINEHRTVWGVMTMCRVLNVARAGFYAWLHNPVSARDKDNQRLLMLIRDSYSLSGGVYGYRRVH
GDLNEIGETCGKNRVGRIMQLNRIKAVRGYKAPRRIAGRPSVVAPNRVQRQFTVVRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRE
LALDALMMAVWRRKPDGEVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDYIEVFYNRARRH
SHLGGVSPEAFEQASS
GDLNEIGETCGKNRVGRIMQLNRIKAVRGYKAPRRIAGRPSVVAPNRVQRQFTVVRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRE
LALDALMMAVWRRKPDGEVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDYIEVFYNRARRH
SHLGGVSPEAFEQASS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1136 bp | 399 aa | 101 | 1236 | + | Yes |
Chemistry : DDE
ORF sequence :
MGIPRFTPEFKEEAVRQITERGYSVAEVSDRLGVSAHSLYKWLRAIKPDNSEQHARDLLEAKSEILKLRAQLKRTEEERDILKKRDPETPGAAKTHRRRT
GYPEKGRAVLCKGARLKYRFINEHRTVWGVMTMCRVLNVARAGFYAWLHNPVSARDKDNQRLLMLIRDSYSLSGGVYGYRRVHGDLNEIGETCGKNRVGR
IMQLNRIKAVRGYKAPRRIAGRPSVVAPNRVQRQFTVVRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRELALDALMMAVWRRKPDG
EVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDYIEVFYNRARRHSHLGGVSPEAFEQASS
GYPEKGRAVLCKGARLKYRFINEHRTVWGVMTMCRVLNVARAGFYAWLHNPVSARDKDNQRLLMLIRDSYSLSGGVYGYRRVHGDLNEIGETCGKNRVGR
IMQLNRIKAVRGYKAPRRIAGRPSVVAPNRVQRQFTVVRANQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWSMKPTLSRELALDALMMAVWRRKPDG
EVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYKTRDLARADIFDYIEVFYNRARRHSHLGGVSPEAFEQASS
Blast result :
Comments
Genome in progress.
ISEc14 is 70% (ORFA) and 74% (ORFB) aa similar to ISPca2. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISEc14 is 70% (ORFA) and 74% (ORFB) aa similar to ISPca2. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2007) Direct Submission GenBank.