ISAtu4
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003064 | ND | Agrobacterium tumefaciens | Agrobacterium tumefaciens C58 |
DNA section
IS Length : 1241 bp
Ends
IR Length : 39/50
IRL : TGGCCTGCTGCCGGTTTTATGGACACCGAGTTAAGGTGTTCCTCCTGAAA
IRR : TGGCCTGCTGCCGGTTTATCGGACACGAGGTTAGGCTAATCCCACCTTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGGTTCCTT | GTAGGCCAGC | 0 |
DNA sequence
TGGCCTGCTGCCGGTTTTATGGACACCGAGTTAAGGTGTTCCTCCTGAAACCGGAGAGCATGAATGCAAAGACGAATATTCAGCCGCGAGTACAAGCTTG
AGGCGGTGAAGCTGGTCAGGGAACGCGGGGTCACTGTTGCGCAGGCTGCCCGTGACCTTGATGTCCACGAGAATGTGTTGCGCAAATGGGTTCGGGAATA
CGGCGACGATCCAAGCCAGTCCTTTCCTGGAAAAGGGCAGATGAAGCCCGAGCAGCTTGAGATCGAGCGGCTCCGTCGTGAAGTCACAAAGCTGAAGGCG
GAGCGCGATATCCTAAAAAAGGCCGCAGCCTACTTTGCCAAGGACGCGATATGAAGTTCGCGTTCATTGCAAAGCACCGTTCGATCTGGCCGGTGGCATG
GCTGTGCGAAGCGTTGGGAGTTTCCCGTTCAGGCTTTCATGCCTGGTTCAATCGTTCTCCGAGCGAACACGCACGCCATGATGAAGTGCTACTAGAAAGG
ATCAAGCAGAGCTTCCAGTCCAGTGACCGCACTTATGGTGCCCGACGGGTCTGGCACGATGTGCTGGAAGAAGGATTATCCTGTGGCCTCCATCGCATTG
AGCGGCTGATGCGCTTGAATGCGCTCAGGGCAAGGCCGAGGCGTCGTGGTTTTCCAAAGGATGCGGGTGACCGCCCCGTCGTCATGCCTAACGTGCTGGA
CCGTCAGTTCGTGGCAGATCGGCCCAACCAAAAATGGGTGGCCGACTTCACCTATATCTGGACCGCAGAGGGTTGGCTGTATGTCGCGGCTGTCATTGAC
CTGTTCTCGCGCCGTGTCGTCGGCTGGTCGATGAATGCCAACATGACAGCCCAGCTCGTCACGGACGCGCTGATCATGGCGATCTGGCGTCGAGGAAAAC
CGGATGCTCTGTTGCATCATTCCGATCAGGGAAGCCAATATACGAGCGAGCAGTTCCAGCGCCTCATGGGCGACCACGGCATCACCTGCTCGATGAGCCG
CTCTGGAAATGTCTGGGACAACGCCGCAATGGAGAGCTTCTTCTCATCATTGAAAACGGAGAGAACGGCACGAAAGGTCTATCGCACGAGGAACGACGCC
AGAGCGGATGTGTTCGATTACATCGAGCGCTTCTACAATCCCAAGCGTCGCCACTCGACACTGGGCTATCTCAGCCCCGATGACTTTGAAACAAAGGTGG
GATTAGCCTAACCTCGTGTCCGATAAACCGGCAGCAGGCCA
AGGCGGTGAAGCTGGTCAGGGAACGCGGGGTCACTGTTGCGCAGGCTGCCCGTGACCTTGATGTCCACGAGAATGTGTTGCGCAAATGGGTTCGGGAATA
CGGCGACGATCCAAGCCAGTCCTTTCCTGGAAAAGGGCAGATGAAGCCCGAGCAGCTTGAGATCGAGCGGCTCCGTCGTGAAGTCACAAAGCTGAAGGCG
GAGCGCGATATCCTAAAAAAGGCCGCAGCCTACTTTGCCAAGGACGCGATATGAAGTTCGCGTTCATTGCAAAGCACCGTTCGATCTGGCCGGTGGCATG
GCTGTGCGAAGCGTTGGGAGTTTCCCGTTCAGGCTTTCATGCCTGGTTCAATCGTTCTCCGAGCGAACACGCACGCCATGATGAAGTGCTACTAGAAAGG
ATCAAGCAGAGCTTCCAGTCCAGTGACCGCACTTATGGTGCCCGACGGGTCTGGCACGATGTGCTGGAAGAAGGATTATCCTGTGGCCTCCATCGCATTG
AGCGGCTGATGCGCTTGAATGCGCTCAGGGCAAGGCCGAGGCGTCGTGGTTTTCCAAAGGATGCGGGTGACCGCCCCGTCGTCATGCCTAACGTGCTGGA
CCGTCAGTTCGTGGCAGATCGGCCCAACCAAAAATGGGTGGCCGACTTCACCTATATCTGGACCGCAGAGGGTTGGCTGTATGTCGCGGCTGTCATTGAC
CTGTTCTCGCGCCGTGTCGTCGGCTGGTCGATGAATGCCAACATGACAGCCCAGCTCGTCACGGACGCGCTGATCATGGCGATCTGGCGTCGAGGAAAAC
CGGATGCTCTGTTGCATCATTCCGATCAGGGAAGCCAATATACGAGCGAGCAGTTCCAGCGCCTCATGGGCGACCACGGCATCACCTGCTCGATGAGCCG
CTCTGGAAATGTCTGGGACAACGCCGCAATGGAGAGCTTCTTCTCATCATTGAAAACGGAGAGAACGGCACGAAAGGTCTATCGCACGAGGAACGACGCC
AGAGCGGATGTGTTCGATTACATCGAGCGCTTCTACAATCCCAAGCGTCGCCACTCGACACTGGGCTATCTCAGCCCCGATGACTTTGAAACAAAGGTGG
GATTAGCCTAACCTCGTGTCCGATAAACCGGCAGCAGGCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 64 | 354 | + | No |
Description : First part of the transposase
ORF sequence :
MQRRIFSREYKLEAVKLVRERGVTVAQAARDLDVHENVLRKWVREYGDDPSQSFPGKGQMKPEQLEIERLRREVTKLKAERDILKKAAAYFAKDAI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 282 | 1211 | + | No |
Description : Second part of the transposase
ORF sequence :
SHKAEGGARYPKKGRSLLCQGRDMKFAFIAKHRSIWPVAWLCEALGVSRSGFHAWFNRSPSEHARHDEVLLERIKQSFQSSDRTYGARRVWHDVLEEGLS
CGLHRIERLMRLNALRARPRRRGFPKDAGDRPVVMPNVLDRQFVADRPNQKWVADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMNANMTAQLVTDALIMA
IWRRGKPDALLHHSDQGSQYTSEQFQRLMGDHGITCSMSRSGNVWDNAAMESFFSSLKTERTARKVYRTRNDARADVFDYIERFYNPKRRHSTLGYLSPD
DFETKVGLA
CGLHRIERLMRLNALRARPRRRGFPKDAGDRPVVMPNVLDRQFVADRPNQKWVADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMNANMTAQLVTDALIMA
IWRRGKPDALLHHSDQGSQYTSEQFQRLMGDHGITCSMSRSGNVWDNAAMESFFSSLKTERTARKVYRTRNDARADVFDYIERFYNPKRRHSTLGYLSPD
DFETKVGLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 64 | 1211 | + | Yes |
Chemistry : DDE
ORF sequence :
MQRRIFSREYKLEAVKLVRERGVTVAQAARDLDVHENVLRKWVREYGDDPSQSFPGKGQMKPEQLEIERLRREVTKLKAERDILKKGRSLLCQGRDMKFA
FIAKHRSIWPVAWLCEALGVSRSGFHAWFNRSPSEHARHDEVLLERIKQSFQSSDRTYGARRVWHDVLEEGLSCGLHRIERLMRLNALRARPRRRGFPKD
AGDRPVVMPNVLDRQFVADRPNQKWVADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMNANMTAQLVTDALIMAIWRRGKPDALLHHSDQGSQYTSEQFQR
LMGDHGITCSMSRSGNVWDNAAMESFFSSLKTERTARKVYRTRNDARADVFDYIERFYNPKRRHSTLGYLSPDDFETKVGLA
FIAKHRSIWPVAWLCEALGVSRSGFHAWFNRSPSEHARHDEVLLERIKQSFQSSDRTYGARRVWHDVLEEGLSCGLHRIERLMRLNALRARPRRRGFPKD
AGDRPVVMPNVLDRQFVADRPNQKWVADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMNANMTAQLVTDALIMAIWRRGKPDALLHHSDQGSQYTSEQFQR
LMGDHGITCSMSRSGNVWDNAAMESFFSSLKTERTARKVYRTRNDARADVFDYIERFYNPKRRHSTLGYLSPDDFETKVGLA
Blast result :
Comments
ISAtu4 is 72 % (ORFA) aa similar to ISLpn7 and 64% (ORFB) aa similar to ISAisp2. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Goodner,B., Hinkle,G., Gattung,S., Miller,N., Blanchard,M., Qurollo,B., Goldman,B.S., Cao,Y., Askenazi,M., Halling,C., Mullin,L., Houmiel,K., Gordon,J., Vaudin,M., Iartchouk,O., Epp,A., Liu,F., Wollam,C., Allinger,M., Doughty,D., Scott,C., Lappas,C., Markelz,B., Flanagan,C., Crowell,C., Gurson,J., Lomo,C., Sear,C., Strub,G., Cielo,C. and Slater,S. (2001) Science 294 (5550), 2323-2328