ISAzvi10

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001157 ND Azotobacter vinelandii
Azotobacter vinelandii AvOP
DNA section
IS Length : 1431 bp

Ends


IR Length : 19/21

IRL : TAGCACTACAGTTGCGTTTTTGCAGCTTATCTGTCCTGATGATCTTCCTT
IRR : TAGCACTACAGTTGTATTTTTCTTCTCATGTGTGCTTGTTGGGCCCTGTG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TAGCACTACAGTTGCGTTTTTGCAGCTTATCTGTCCTGATGATCTTCCTTCTGGCGAGGAGATTTCAGATGACTCACGCTTCCATCGAAACCACTCTGGA
GCTATGGGTCTCGTCGCTGCGGGACGTCAAGCAGCGCATCAGACCCCTGTTCACTCAGGAGCGCGTAGCCGCTTCTGCAGCCTTGTTTCTGGATGGCCTG
TTGGGTGCCGAGCCACGCAAGACCGGTTGGATGCGTGCCGAGGCGGCGGGCGATCCCGGCCCCTGGCGACAACAGGCCATTCTGGGGCGCGGCCGCTGGG
CGGCCGATGCCTTGCGCGATCTGGTTCGCGACTATGTGCTCGAGCAATTGGCCGACGACGAAGCTGTTCTGGTTCTGGACGAGACGGGCTTTCTCAAGCA
AGGCCATACCTCTTGTGGCGTTGCTCGCCAGTACACCGGCTCGGCCGGCAAGATCACCAATTGCCAGATCGGTGTCTTTGCCGCTTATGTCTCGCGCCAC
GGACAGGCCTTCATCGACCGAGCGCTGTATCTCCCCAGGGACTGGACGGCAGACCCCGAACGCCTGGCAGCCGCCCATGTGCCGAAGGGCATCGGATTCT
CGACCAAACCCCGGCTGGCTCAGGACATGATCGAGCGGGCCATCGCAGCGAATACGCCGTTCGCCTGGGTGGCCGCCGACAGCGTCTATGGAGTGGGTCA
TCTCGAGACCGCCCTGCGACGTGCCGGGAAGGGCTACGTGTTGGGTGTCAAGGGCACCCATGCGGTCAAGGCCTGGATTGACCGGCCCTGGCTCTCCGGC
ACGGCCGAACAGGCGGCTCGGGCGTTACCATCTTCGGCCTGGAGGCGCTGCTCGGCAGGCGAAGGCACTCAGGGGCCACGCCTGCACGAGTGGGCCTATC
TGGAGCTGGCGGACCTGGAGGCCAGTGACTATGCCCCCTCCGCAACGGGCGTCTGGACACGGGGCCTGCTGATCCGTCGCCGTCTGGTTGACGGAGAACT
GGCCTATTTCACCACCTGGGCACCGGCTGGAACCTCTCTGAAGAGGCTGGCCTGGGTGGAGGGCCATCGCTGGTCGATCGAGAGCGCCTTCGAAACGGCC
AAGGGCGAGTTGGGCCTGGATCACAACGAGACCCGCTCCTGGCATGGCTGGCATCGCCATGTCTCGCTGGTCATGCTGGCCTTTGCCTTGTTGGCCCGGG
TGCGCCATCAGGCCAACGCCCCGGCTCCCCCAAAAACCATCCAGCGGCTGATAGCCCTGACATATCCGGGTTGATCCGCTGGTCCATGCGGGAAATCCGG
CGAATGGCTACCCGACTGGCACAACGGCAAATCGCACCGGATCACATCATGGCTTGGTCGCTCTGGCGCAGGAGACATCAGCACAGGGCCCAACAAGCAC
ACATGAGAAGAAAAATACAACTGTAGTGCTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1203 bp401 aa691271+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTHASIETTLELWVSSLRDVKQRIRPLFTQERVAASAALFLDGLLGAEPRKTGWMRAEAAGDPGPWRQQAILGRGRWAADALRDLVRDYVLEQLADDEAV
LVLDETGFLKQGHTSCGVARQYTGSAGKITNCQIGVFAAYVSRHGQAFIDRALYLPRDWTADPERLAAAHVPKGIGFSTKPRLAQDMIERAIAANTPFAW
VAADSVYGVGHLETALRRAGKGYVLGVKGTHAVKAWIDRPWLSGTAEQAARALPSSAWRRCSAGEGTQGPRLHEWAYLELADLEASDYAPSATGVWTRGL
LIRRRLVDGELAYFTTWAPAGTSLKRLAWVEGHRWSIESAFETAKGELGLDHNETRSWHGWHRHVSLVMLAFALLARVRHQANAPAPPKTIQRLIALTYP
G

 

Blast result :
Comments
ISAzvi10 is 70% aa similar to IS1357. ISAzvi10 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS1452 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-133-E(C1). The copy number of ISAzvi10 in Azotobacter vinelandii AvOP is unknown since genome sequencing is still in progress. Genome in progress : current accession number : NZ_AAAU03000001.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.Unpublished
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18