ISfinder Mirror Site

ISUnCu8

Family IS4
Group IS50

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	uncultured bacterium	uncultured bacterium

DNA section

IS Length : 1509 bp

Ends

IR Length : 15/20

IRL : CTGTCTCTCATACACAAATCCCTGAACAATGCTCAGGGATTTTTTTTGAA
IRR : CTGTCTCTTGATCACAAGTCTTCGTGATTGAGAGACATGGCCAATTCGTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

CTGTCTCTCATACACAAATCCCTGAACAATGCTCAGGGATTTTTTTTGAACTCATTCCCCGCATTGTGATCAGATCGTCTAGGGCTAAATATTCGAGGCG
ATTATGATGAAGATGATGGATCCAGAGCAGTGGGCGCAATGTCAATTTGGGCACGCAAACCTCAACGACCCAAGACGTACACAAAGACTTGTTTCACTGG
CTACCTCAATTACCCAACAACCCGGAGTTGCCGTGTCCAAACTTCCCTTATCCCCCGCTGAGATGGAAGGGGCCTACCGATTTATCCGCAATGAGAATAT
TCAAGCCAGTGATATTGCTGAAGCGGGTTTTCAAGCCACCGCGCAGCAAGCTCGAGAGCATGACATCTTGTTAGCACTAGAGGATACCACCTCGCTGACT
TACAAGCATGACAGTGTCAGGGAGGAGTTAGGTCATACCAATCAAGGGGATAAAAACCGTGCCATTCTGGCGCATTCTATCTTGCTGTTTTCACCGCAAA
GCCAGCAAGTCGTTGGGCTGATTGAGCAACAGCGTTGGACGCGGGATATCACTAAGCGCGGGCAACGCCGCCAACATGCTACTCGCCCTTACGAGGAAAA
AGAGAGCTACAAATGGGAACAAGCCTCCGCCAATATGTCTGCCCGTTTAGGCGAGCATATCAACAAGGTGATTTCCGTCTGTGACCGTGAGGCTGATTTG
TTTGAATATCTGACCTATAAAACCCAGCACCAACAGCGCTTTGTCGTCCGCTCGATGCAGAGCCGATGCATTCAAGAGCATGCACACAAACTCTATGACT
ACTCGAACACATTACCGCTTGCAGCGACGAAATCTCTCCTCATTCCTCAGAAAGGCGGAAGGAAAGCCCGGGCGGTGACGCTGGAAATGAGATATGCCCG
AGTGACCCTCAAAGCTCCAGCCAACAAACGCACTCAGGCAGACATCCCTCTGTATTACGTCAGTGTCGTAGAGCAAAGTCATCGTGAAGAAAAACTGGCA
TGGCACTTACTCACCTCAGAACCTATCACATGCTCGAAGGAGGCACTGGAGGTTGTCGGCTATTACGAGCGCCGTTGGTTGATTGAAGATTACCACAAGG
TATGGAAAAGCAGTGGCACCGCGGTGGAAGAGCTGAGAATGCAGTGCCGTGAGAATCTGGAGCGTATGAGTGTGATACTGGCTTTTATCGCCACACGCTT
ACTCCAGCTTCGCTTTATGAAGGTATCCAAAGCGGAAGTGGCAGCACAATGTTGTGAAACGCTATTGGGTCAGAAAGCGTGGAAATTACTTTGGCTGAAA
ATGGAGGGACGTCCGTTACCTCAACAGGCACCCGATATGCAGTGGGCGTACGAGCGTCTGGCCAGATTAGGGGGTTGGAAAGATACCAAGCGGACAGGTC
GAGCTTGTGTAGAGGTATTGTGGGAAGGATGGTTTAGGTTACAAACCATCCTTGAGGGTTACGAATTGGCCATGTCTCTCAATCACGAAGACTTGTGATC
AAGAGACAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1380 bp	460 aa	116	1495	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDPEQWAQCQFGHANLNDPRRTQRLVSLATSITQQPGVAVSKLPLSPAEMEGAYRFIRNENIQASDIAEAGFQATAQQAREHDILLALEDTTSLTYKHDS
VREELGHTNQGDKNRAILAHSILLFSPQSQQVVGLIEQQRWTRDITKRGQRRQHATRPYEEKESYKWEQASANMSARLGEHINKVISVCDREADLFEYLT
YKTQHQQRFVVRSMQSRCIQEHAHKLYDYSNTLPLAATKSLLIPQKGGRKARAVTLEMRYARVTLKAPANKRTQADIPLYYVSVVEQSHREEKLAWHLLT
SEPITCSKEALEVVGYYERRWLIEDYHKVWKSSGTAVEELRMQCRENLERMSVILAFIATRLLQLRFMKVSKAEVAAQCCETLLGQKAWKLLWLKMEGRP
LPQQAPDMQWAYERLARLGGWKDTKRTGRACVEVLWEGWFRLQTILEGYELAMSLNHEDL

Blast result :

Comments

ISUnCu8 is 61% aa similar to ISPpr3. ISUnCu8 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS50 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-133-E(C1).
Accession number : AACY01017159.
ISUnCu8 is from uncultured bacterium (environmental sequence from Sargasso Sea).

References

1] Venter,J.C., Remington,K., Heidelberg,J.F., Halpern,A.L., Rusch,D., Eisen,J.A., Wu,D., Paulsen,I., Nelson,K.E., Nelson,W., Fouts,D.E., Levy,S., Knap,A.H., Lomas,M.W., Nealson,K., White,O., Peterson,J., Hoffman,J., Parsons,R., Baden-Tillson,H., Pfannkoch,C., Rogers,Y.H. and Smith,H.O. (2004) Science 304 (5667), 66-74
2] Reznikoff, W.S., Bordenstein, S.R. and Apodaca, J. (2004) J. Bact. 186(24), 8240-8247
3] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18