ISRel1

  • Family IS4
  • Group IS4Sa
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007762 ND Rhizobium etli
Rhizobium etli CFN 42
DNA section
IS Length : 1273 bp

Ends


IR Length : 22

IRL : CACTGCTGTCCGGTTTAAATTTAGTGGACATGGTGCACGGTGTTGATTCT
IRR : CACTGCTGTCCGGTTTAAATTTTGTCACCATTGGAGGCTCATCTGGCCGG

Insertion site


Left flankDirect repeatRight flankDR Length
GTCATGGTCTCTCCTGTTCCCGGCATCTA9

DNA sequence

CACTGCTGTCCGGTTTAAATTTAGTGGACATGGTGCACGGTGTTGATTCTACTCATGTTCGAGCGTTTCGCGACGATCTCGGACACGAAAGAGGAACAAC
ACCGTGCGGCATGACAATAGCGTCTTTCATGATCTGTTGAAGCGGATTCCGTGGACGGTGTTCGAAAGACTTGTGGAGGAGCATCAGGCCGACAAGCACG
TTCGGCGGCTGTCGACGAAGAGCCAGTTGATCGCGCTGCTTTACGGCCAGCTTGCCGGTGCCGTCAGCCCGCGCGAGATCGTCGGCTCCCTGGAAAGCCA
TAGTGCCCGCCTTTACCATCTCGGCGCTCGCCCGGTGTCGCGCTCGACCTTCGCCGATGCCAACGGCCTGCGTCCGAGCGCCGTTTTTGCAGAGTTGTTC
ACGCAGATGCTGGCCCGCGCCGGGCGCGGCCTCAAGCGGGCCGTCGGCGAGGCGGTCTATCTGATCGACGGCAGCAGTCTGCCACTTTCCGGGGCGGGAT
CGCAGTGGGCCCGCTTTTCCGATCAGGCCTGCGGCGCCAAGATGCACGTCGTCTATGATGCCAAGGCCGAACGGCCGATCTACGCGGCCGTCACCCCGGC
CAATGTCAACGACATCACCGCCGCCAAGGAGATGCCGATCGAGGCCGGCGCCACCTATGTCTTCGACCTCGGCTATTACGACTTCGGCTGGTGGGCGAAG
CTGGACGCCGCCGGCTGCCGCATCGTCAGCCGCCTAAAGTCCCACACGAAACTGACGGTGAGCGCCGAGCAGGCGGCAAACGAGGATGCCGGCATCCTGT
TCGACCGCATCGGCCTGTTGCCGCAGCGTCAGGCCAAGAGCCGCCGCAACCCGATGAACCGGCCGGTGCGCGAGATCGGCGAGCGGATCGAAACCGGCAA
GGTGCTGCGCATCTTCTCCAACGATCTTACTGCCCCGGCCGAGGAGATCGCCGCGCTTTACAAGCGCCGCTGGGCGATCGAACTGTTTTTCCCCTGGGTC
AAGCAGACGCTGAAGATCCGCCATTTCCTCGGCAACAGCGAAAATGCCGTGCGCATCCAGGTGGCCGTCGCTCTGATCGCCTATCTGCTGCTGCAGATGG
CAAAGGCCGACCAGGCCACCATCATAAGCCCGCTGGCCTTCGCCCGCCTGGTGCGCACCAACCTGATGCACCGCAAAAGGATCGACCGCCTGCTAAAGCC
ACGCCACAGCCCTCCCGGAAATCCCGGCCAGATGAGCCTCCAATGGTGACAAAATTTAAACCGGACAGCAGTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1143 bp381 aa1041246+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRHDNSVFHDLLKRIPWTVFERLVEEHQADKHVRRLSTKSQLIALLYGQLAGAVSPREIVGSLESHSARLYHLGARPVSRSTFADANGLRPSAVFAELFT
QMLARAGRGLKRAVGEAVYLIDGSSLPLSGAGSQWARFSDQACGAKMHVVYDAKAERPIYAAVTPANVNDITAAKEMPIEAGATYVFDLGYYDFGWWAKL
DAAGCRIVSRLKSHTKLTVSAEQAANEDAGILFDRIGLLPQRQAKSRRNPMNRPVREIGERIETGKVLRIFSNDLTAPAEEIAALYKRRWAIELFFPWVK
QTLKIRHFLGNSENAVRIQVAVALIAYLLLQMAKADQATIISPLAFARLVRTNLMHRKRIDRLLKPRHSPPGNPGQMSLQW

 

Blast result :
Comments
ISRel1 is 37% aa similar to ISRm16. ISRel1 has been found by screening completely sequenced bacterial genomes for sequences homologous to the ISAva1 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-104-E(C1). The copy number of ISRel1 in Rhizobium etli CFN 42 (plasmid p42a) is 1. On p42a, an insertion sequence of the IS21 family is inserted into ISRel1. The present sequence was reconstructed by in silico deletion of the IS21 insertion together with one copy of the IS21 direct repeat.
References
1] Gonzalez,V., Santamaria,R.I., Bustos,P., Hernandez-Gonzalez,I., Medrano-Soto,A., Moreno-Hagelsieb,G., Janga,S.C., Ramirez,M.A., Jimenez-Jacinto,V., Collado-Vides,J. and Davila,G. (2006) Proc Natl Acad Sci USA Mar 7;103(10):3834-9
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18