ISMasp3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008576 | ND | Magnetococcus sp. | Magnetococcus sp. MC-1 |
DNA section
IS Length : 1258 bp
Ends
IR Length : 21/24
IRL : TGATGGGGCTACACTTTAACGGACACAAAAAATGAGGGAAAATGAGACCT
IRR : TGATGTGGCAACACTAAAACGGACAGTGTTTTACGCGGACTTGAACCACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATACCCAGCC | GCTGCCACAG | 0 |
DNA sequence
TGATGGGGCTACACTTTAACGGACACAAAAAATGAGGGAAAATGAGACCTCATTCAGAAAGGTGTCCAATGAGCAGAAGAAGTCCTAAAAATTTTGCCCC
AGAGTTCAAAGAGCAGGTGCTCAAAATGGTAGAGAGCTCTGACAAGCCCATACCAGTGATTGCTGAGGAGTTTGGCATATCAGCTAAGAATATCTACAAC
TGGAGAAAGCGATCTCATGAGAAGCTAACATCCAGAGATAAAGATGAAATATTAGAAGTGAAAGAAGAAAATAAGCGCCTTAAAAAGAAGCTGGCTCAGC
TTGAGGAGGAGCAGGCGATATTAAAAAAGGCGTGTGCGTACTTCGCGAGATCCCTTCCGTGAGGTACGCCTTTATTCAAGCTCATAGCAAAAGCTACTCT
GTCGAGTCTTTATGCCGTGTTCTGGATGTTTCTCGTAGTGGATTTTATGGCTGGCTTAATCGAACCAGTACGAGTAAGGCTGAAGAGAATCAACTGCTTG
AAGAGTGGATAGCAGAGATTTTCAAGGTGAGTCGATCAACCTATGGAACACGGCGTATACGCAAAGCGTTAAATGAGCATGGTGTACGAGTGAGTCGCCG
ACGCGTCTCCAGAATCAAGAGGAAGCTTGGCTTGGTTTGTAAAGCTCAGAAGCGTTATAAGGTTGTAACGACAGATTCAAATCACGCACTGCCTATAGCG
CCTAACCTTCTTGATCGTCAATTCGATACGGAACGCCCTGATAACGCCTATGTTGGTGACATCACATATATTCCGACCAAGGAAGGTTGGCTTTATCTGG
CTGTTTGGATTGACCTTTATTCCAGAGCTGTTGTCGGTTGGTCTATGGCAGACCCTATGAAAGCAGAACTGGTAACAGACGCTTTACGCATGGCCTTTTT
CAAGCGCCGCCCAGAGGCCGGGCTGATTGTTCATAGTGATCGTGGTAGCCAATATGCATCTGAACTCTTGAGAAAATATCTGGATGAAAGAGGCTATACA
CAGAGTATGAGTCGCCGTGGTAACTGTTGGGACAACGCACCAGCAGAAAGCTTTTTCCACACTCTCAAAGTTGAACTGGTTTCATCCTGTGACTTTAAGA
CACGCGATGAGGCTAAACAGATCATCTTTGAATATATAGAGGTCTTTTATAACCGAAAGCGCAAGCACTCATCCATCGGCTATATGAGTCCTTCTCAGTA
CGAAATAGAGTGGTTCAAGTCCGCGTAAAACACTGTCCGTTTTAGTGTTGCCACATCA
AGAGTTCAAAGAGCAGGTGCTCAAAATGGTAGAGAGCTCTGACAAGCCCATACCAGTGATTGCTGAGGAGTTTGGCATATCAGCTAAGAATATCTACAAC
TGGAGAAAGCGATCTCATGAGAAGCTAACATCCAGAGATAAAGATGAAATATTAGAAGTGAAAGAAGAAAATAAGCGCCTTAAAAAGAAGCTGGCTCAGC
TTGAGGAGGAGCAGGCGATATTAAAAAAGGCGTGTGCGTACTTCGCGAGATCCCTTCCGTGAGGTACGCCTTTATTCAAGCTCATAGCAAAAGCTACTCT
GTCGAGTCTTTATGCCGTGTTCTGGATGTTTCTCGTAGTGGATTTTATGGCTGGCTTAATCGAACCAGTACGAGTAAGGCTGAAGAGAATCAACTGCTTG
AAGAGTGGATAGCAGAGATTTTCAAGGTGAGTCGATCAACCTATGGAACACGGCGTATACGCAAAGCGTTAAATGAGCATGGTGTACGAGTGAGTCGCCG
ACGCGTCTCCAGAATCAAGAGGAAGCTTGGCTTGGTTTGTAAAGCTCAGAAGCGTTATAAGGTTGTAACGACAGATTCAAATCACGCACTGCCTATAGCG
CCTAACCTTCTTGATCGTCAATTCGATACGGAACGCCCTGATAACGCCTATGTTGGTGACATCACATATATTCCGACCAAGGAAGGTTGGCTTTATCTGG
CTGTTTGGATTGACCTTTATTCCAGAGCTGTTGTCGGTTGGTCTATGGCAGACCCTATGAAAGCAGAACTGGTAACAGACGCTTTACGCATGGCCTTTTT
CAAGCGCCGCCCAGAGGCCGGGCTGATTGTTCATAGTGATCGTGGTAGCCAATATGCATCTGAACTCTTGAGAAAATATCTGGATGAAAGAGGCTATACA
CAGAGTATGAGTCGCCGTGGTAACTGTTGGGACAACGCACCAGCAGAAAGCTTTTTCCACACTCTCAAAGTTGAACTGGTTTCATCCTGTGACTTTAAGA
CACGCGATGAGGCTAAACAGATCATCTTTGAATATATAGAGGTCTTTTATAACCGAAAGCGCAAGCACTCATCCATCGGCTATATGAGTCCTTCTCAGTA
CGAAATAGAGTGGTTCAAGTCCGCGTAAAACACTGTCCGTTTTAGTGTTGCCACATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 69 | 362 | + | No |
Description : First part of the transposase
ORF sequence :
MSRRSPKNFAPEFKEQVLKMVESSDKPIPVIAEEFGISAKNIYNWRKRSHEKLTSRDKDEILEVKEENKRLKKKLAQLEEEQAILKKACAYFARSLP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 305 | 1228 | + | No |
Description : Second part of the transposase
ORF sequence :
GGAGDIKKGVCVLREIPSVRYAFIQAHSKSYSVESLCRVLDVSRSGFYGWLNRTSTSKAEENQLLEEWIAEIFKVSRSTYGTRRIRKALNEHGVRVSRRR
VSRIKRKLGLVCKAQKRYKVVTTDSNHALPIAPNLLDRQFDTERPDNAYVGDITYIPTKEGWLYLAVWIDLYSRAVVGWSMADPMKAELVTDALRMAFFK
RRPEAGLIVHSDRGSQYASELLRKYLDERGYTQSMSRRGNCWDNAPAESFFHTLKVELVSSCDFKTRDEAKQIIFEYIEVFYNRKRKHSSIGYMSPSQYE
IEWFKSA
VSRIKRKLGLVCKAQKRYKVVTTDSNHALPIAPNLLDRQFDTERPDNAYVGDITYIPTKEGWLYLAVWIDLYSRAVVGWSMADPMKAELVTDALRMAFFK
RRPEAGLIVHSDRGSQYASELLRKYLDERGYTQSMSRRGNCWDNAPAESFFHTLKVELVSSCDFKTRDEAKQIIFEYIEVFYNRKRKHSSIGYMSPSQYE
IEWFKSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 69 | 1228 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRRSPKNFAPEFKEQVLKMVESSDKPIPVIAEEFGISAKNIYNWRKRSHEKLTSRDKDEILEVKEENKRLKKKLAQLEEEQAILKKGVCVLREIPSVRY
AFIQAHSKSYSVESLCRVLDVSRSGFYGWLNRTSTSKAEENQLLEEWIAEIFKVSRSTYGTRRIRKALNEHGVRVSRRRVSRIKRKLGLVCKAQKRYKVV
TTDSNHALPIAPNLLDRQFDTERPDNAYVGDITYIPTKEGWLYLAVWIDLYSRAVVGWSMADPMKAELVTDALRMAFFKRRPEAGLIVHSDRGSQYASEL
LRKYLDERGYTQSMSRRGNCWDNAPAESFFHTLKVELVSSCDFKTRDEAKQIIFEYIEVFYNRKRKHSSIGYMSPSQYEIEWFKSA
AFIQAHSKSYSVESLCRVLDVSRSGFYGWLNRTSTSKAEENQLLEEWIAEIFKVSRSTYGTRRIRKALNEHGVRVSRRRVSRIKRKLGLVCKAQKRYKVV
TTDSNHALPIAPNLLDRQFDTERPDNAYVGDITYIPTKEGWLYLAVWIDLYSRAVVGWSMADPMKAELVTDALRMAFFKRRPEAGLIVHSDRGSQYASEL
LRKYLDERGYTQSMSRRGNCWDNAPAESFFHTLKVELVSSCDFKTRDEAKQIIFEYIEVFYNRKRKHSSIGYMSPSQYEIEWFKSA
Blast result :
Comments
ISMasp3 is 58 % (ORFA) aa similar to IS3 and 68% (ORFB) aa similar to ISApr1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Goodwin,L.A., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N. and Richardson,P. (2006) Direct submission GenBank.