ISRme12
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007973. | ND | Ralstonia metallidurans | Ralstonia metallidurans CH34 |
DNA section
IS Length : 1454 bp
Ends
IR Length : 23/26
IRL : TGAACTGCACCCCAAAGGTTGGACACCCGTTCAACCTTTGGGGTGTTTTT
IRR : TGAACTGACCCCAAGAAGTTGGACAGTTAAGGCGGTAGGCTAAGCCATAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAGCCCGCG | TAAC | TGCGGGCTTT | 4 |
DNA sequence
TGAACTGCACCCCAAAGGTTGGACACCCGTTCAACCTTTGGGGTGTTTTTCATGGCGAAGTACGATGAGAGCTTCAAGCGCCAGGTTGTTGAGCGCTATC
TGACAGGATCTTCCGGTTTCAAGGCCTTGGGCAAGGAACTGGGTGTGGACCCGGGAGCCATACGGCGCTGGGTTGGCAGCTATGCTCATCACGGTGCAGC
AGGGCTGAGAAAGAAATCTGTCCGGTATGGCGCGAAGTTCAAATTGTCGGTGCTACGGCGGATGTGGCGCGACGAGTTGTCCTACCGACAGGTTGCTGCT
CTGTTCGATCTGAGGGGCGGGACTGCTGTCGTCTCGGCGTGGGAACGCCAGTATCATTCGCAGGGAATAGACGCACTGGCGCCCAAACCACGAGGGCGTA
CGAAGACAATGTCGGCTCCTATACCTCCCAAGCCACCGGCCTCGAATGTCAAAGAGACCCGCACGCTGGAAGATCTGCGTAAAGAGAACGAATACCTGCG
CGCGGAGGTGGCGTACCTAAAAAAATGGAATGCCTTGGTTCAGGCCAAGAAAGCGGCCGCACAGAAAAAGCGCGGCTAGTGCTCGAACTGAGGCAACACT
ATGATTTGGCGGCGCTACTGAAGGTTGCTGGACTGTCGCGCAGCACGTTCTATTACCAGGCCAAGGTTATCGGGGCCGGCGATAGGTACGCCGACTTGAA
GGCGCGTATCCAGAGCATCTACGACCATCATAAGGGCCGTTATGGCTACCGGCGGATCACGGCGGCACTACGACGATCTGGCGAGACGATCAATCACAAG
GTGGTCCAGCGGTTGATGCAGTCACTGGGCCTGAGATCCTTGGTTCGGCCAAAGAAGTACCGCTCATACCGAGGGCCAGGTCACGTGTCCGTGCCTAACC
TGTTGCAGAGGCAGTTCCAAGCCAGGCGCCCGAATGAGAAGTGGGTGACGGACGTGACCGAATTCAATGTCGGCGGCGACAAACTCTACCTCTCGCCTGT
TATGGACCTATACAACGGGGAAATCGTGGCCTATCAGACCAGCCGGCGACCGCACTTCAGCCTGGTTGGCACGATGCTCAGGAAGGCATTGAACAAGCTC
TCCAGTGCTCAACGGCCGCTGCTGCATTCCGATCAGGGTTGGCAATACCAGATGCCGGCCTACCGGCGGGTGCTGGCCGAGCACGGGGTGACAAAAAGCA
TGTCACGTAGGGGAAATTGCCTGGACAACGCCGCCATGGAGAGCTTCTTTGGAACGCTCAAATCCGAATGCTTCTATCTGAGCAAGTTCACCGCCATTGA
CCAGTTGCAGGATGCCTTGCGCCGCTATATCCACTACTACAATCACCACCGCATCAGGACCAAACTAAAAGGGCTGAGTCCTGTGCAATACAGGACTCAG
CCCTCTATGGCTTAGCCTACCGCCTTAACTGTCCAACTTCTTGGGGTCAGTTCA
TGACAGGATCTTCCGGTTTCAAGGCCTTGGGCAAGGAACTGGGTGTGGACCCGGGAGCCATACGGCGCTGGGTTGGCAGCTATGCTCATCACGGTGCAGC
AGGGCTGAGAAAGAAATCTGTCCGGTATGGCGCGAAGTTCAAATTGTCGGTGCTACGGCGGATGTGGCGCGACGAGTTGTCCTACCGACAGGTTGCTGCT
CTGTTCGATCTGAGGGGCGGGACTGCTGTCGTCTCGGCGTGGGAACGCCAGTATCATTCGCAGGGAATAGACGCACTGGCGCCCAAACCACGAGGGCGTA
CGAAGACAATGTCGGCTCCTATACCTCCCAAGCCACCGGCCTCGAATGTCAAAGAGACCCGCACGCTGGAAGATCTGCGTAAAGAGAACGAATACCTGCG
CGCGGAGGTGGCGTACCTAAAAAAATGGAATGCCTTGGTTCAGGCCAAGAAAGCGGCCGCACAGAAAAAGCGCGGCTAGTGCTCGAACTGAGGCAACACT
ATGATTTGGCGGCGCTACTGAAGGTTGCTGGACTGTCGCGCAGCACGTTCTATTACCAGGCCAAGGTTATCGGGGCCGGCGATAGGTACGCCGACTTGAA
GGCGCGTATCCAGAGCATCTACGACCATCATAAGGGCCGTTATGGCTACCGGCGGATCACGGCGGCACTACGACGATCTGGCGAGACGATCAATCACAAG
GTGGTCCAGCGGTTGATGCAGTCACTGGGCCTGAGATCCTTGGTTCGGCCAAAGAAGTACCGCTCATACCGAGGGCCAGGTCACGTGTCCGTGCCTAACC
TGTTGCAGAGGCAGTTCCAAGCCAGGCGCCCGAATGAGAAGTGGGTGACGGACGTGACCGAATTCAATGTCGGCGGCGACAAACTCTACCTCTCGCCTGT
TATGGACCTATACAACGGGGAAATCGTGGCCTATCAGACCAGCCGGCGACCGCACTTCAGCCTGGTTGGCACGATGCTCAGGAAGGCATTGAACAAGCTC
TCCAGTGCTCAACGGCCGCTGCTGCATTCCGATCAGGGTTGGCAATACCAGATGCCGGCCTACCGGCGGGTGCTGGCCGAGCACGGGGTGACAAAAAGCA
TGTCACGTAGGGGAAATTGCCTGGACAACGCCGCCATGGAGAGCTTCTTTGGAACGCTCAAATCCGAATGCTTCTATCTGAGCAAGTTCACCGCCATTGA
CCAGTTGCAGGATGCCTTGCGCCGCTATATCCACTACTACAATCACCACCGCATCAGGACCAAACTAAAAGGGCTGAGTCCTGTGCAATACAGGACTCAG
CCCTCTATGGCTTAGCCTACCGCCTTAACTGTCCAACTTCTTGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 528 bp | 175 aa | 52 | 579 | + | No |
Description : First part of the transposase
ORF sequence :
MAKYDESFKRQVVERYLTGSSGFKALGKELGVDPGAIRRWVGSYAHHGAAGLRKKSVRYGAKFKLSVLRRMWRDELSYRQVAALFDLRGGTAVVSAWERQ
YHSQGIDALAPKPRGRTKTMSAPIPPKPPASNVKETRTLEDLRKENEYLRAEVAYLKKWNALVQAKKAAAQKKRG
YHSQGIDALAPKPRGRTKTMSAPIPPKPPASNVKETRTLEDLRKENEYLRAEVAYLKKWNALVQAKKAAAQKKRG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 483 | 1415 | + | No |
Description : Second part of the transposase
ORF sequence :
RERIPARGGGVPKKMECLGSGQESGRTEKARLVLELRQHYDLAALLKVAGLSRSTFYYQAKVIGAGDRYADLKARIQSIYDHHKGRYGYRRITAALRRSG
ETINHKVVQRLMQSLGLRSLVRPKKYRSYRGPGHVSVPNLLQRQFQARRPNEKWVTDVTEFNVGGDKLYLSPVMDLYNGEIVAYQTSRRPHFSLVGTMLR
KALNKLSSAQRPLLHSDQGWQYQMPAYRRVLAEHGVTKSMSRRGNCLDNAAMESFFGTLKSECFYLSKFTAIDQLQDALRRYIHYYNHHRIRTKLKGLSP
VQYRTQPSMA
ETINHKVVQRLMQSLGLRSLVRPKKYRSYRGPGHVSVPNLLQRQFQARRPNEKWVTDVTEFNVGGDKLYLSPVMDLYNGEIVAYQTSRRPHFSLVGTMLR
KALNKLSSAQRPLLHSDQGWQYQMPAYRRVLAEHGVTKSMSRRGNCLDNAAMESFFGTLKSECFYLSKFTAIDQLQDALRRYIHYYNHHRIRTKLKGLSP
VQYRTQPSMA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1364 bp | 448 aa | 52 | 1415 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKYDESFKRQVVERYLTGSSGFKALGKELGVDPGAIRRWVGSYAHHGAAGLRKKSVRYGAKFKLSVLRRMWRDELSYRQVAALFDLRGGTAVVSAWERQ
YHSQGIDALAPKPRGRTKTMSAPIPPKPPASNVKETRTLEDLRKENEYLRAEVAYLKKWNALVQAKKARLVLELRQHYDLAALLKVAGLSRSTFYYQAKV
IGAGDRYADLKARIQSIYDHHKGRYGYRRITAALRRSGETINHKVVQRLMQSLGLRSLVRPKKYRSYRGPGHVSVPNLLQRQFQARRPNEKWVTDVTEFN
VGGDKLYLSPVMDLYNGEIVAYQTSRRPHFSLVGTMLRKALNKLSSAQRPLLHSDQGWQYQMPAYRRVLAEHGVTKSMSRRGNCLDNAAMESFFGTLKSE
CFYLSKFTAIDQLQDALRRYIHYYNHHRIRTKLKGLSPVQYRTQPSMA
YHSQGIDALAPKPRGRTKTMSAPIPPKPPASNVKETRTLEDLRKENEYLRAEVAYLKKWNALVQAKKARLVLELRQHYDLAALLKVAGLSRSTFYYQAKV
IGAGDRYADLKARIQSIYDHHKGRYGYRRITAALRRSGETINHKVVQRLMQSLGLRSLVRPKKYRSYRGPGHVSVPNLLQRQFQARRPNEKWVTDVTEFN
VGGDKLYLSPVMDLYNGEIVAYQTSRRPHFSLVGTMLRKALNKLSSAQRPLLHSDQGWQYQMPAYRRVLAEHGVTKSMSRRGNCLDNAAMESFFGTLKSE
CFYLSKFTAIDQLQDALRRYIHYYNHHRIRTKLKGLSPVQYRTQPSMA
Blast result :
Comments
ISRme12 is 67% (ORFA) aa similar to ISBcen10 and 79% (ORFB) aa similar to IISBam1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Martinez,M., Goltsman,E., Pitluck,S., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Mergeay,M., Benotmane,M.A., Vallaeys,T., Michaux,A., Monchy,S., Dunn,J., McCorkle,S., Taghavi,S., van der Lelie,D. and Richardson,P. (2006) Direct submission GenBank.