ISAtu3

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003064 ND Agrobacterium tumefaciens
Agrobacterium tumefaciens C58 plasmid At
DNA section
IS Length : 1597 bp

Ends


IR Length : 16/19

IRL : TGCGGACGAAAACTTGGACCACCAGGAGGTAGTTCATGTATTCCTACGCA
IRR : TGCGGATAAAAAGTTGGACTGCTTTATAATTTCAGGCCGAGGCTCTGACG

Insertion site


Left flankDirect repeatRight flankDR Length
CCGCTAGAAGTGGAGGGGGCCCCCTTATCGCGGAAACC0

DNA sequence

TGCGGACGAAAACTTGGACCACCAGGAGGTAGTTCATGTATTCCTACGCAGACAGACTTCGAGCGGTTGAGCTTTATATCCGGCTGGGCAAGCGGCTTAA
CACGACCATTCGCCAGCTGGGATATCCCACCAAGAATGCGCTGAGGGGTTGGTATCGCGAGTATGTGCAGCATCTCGACCTGCGTACTCAGCCGGTAGCG
CGAGCGCCAAAATATTCAGAGGCTCAGAGGCAGGCGGCACTTGAGCACTTTCGCACCCATGATCGTTGCATCTCTGGGACGATGAGGGCGCTCGGCTATC
CCGGTCGAGGAACTCTAACCGCATGGGTTCGTGAGGCTTTTCCGGAGGCGCGGACATCAATGGTCGGTCGATCGTGGCACCCTGGCTATTCCGAGGAGGT
CCGGCAAGCGGGGGTAATCGGACTATGCAGTGGAGATGAAAGTGCTCAGCAGGTAGCTGACCGTCTGGGCGTCTCGAGACCGACATTGTATAGCTGGAAA
GACCAGTTACTCGGTCATGAGGCTTCTTCATCCATGAAACGCCGCAAAGCCAACCCCAAGGTGCCTGAACGTGAAGAACTCGAGCGAAAGCTTGAAGCCC
TCCAACGCGATGTCCGCCAGTTGCAACTCGAACATGATCTCCTGAAAAAGGCCAACGAACTATTAAAAAAAGGTCTGGGCGTCGATCTGCTGATCCTGAG
TAATCGGGAGAAGACACAGCTGATTGACGCCCTCAAGGAAGTTTATCGCTTGCCAGAGCTGCTTGCCCAACTGCGAATTGCCCGAAGCTCGTACTTCTAC
CATCGCGCCCGTATGTGCCTGGCAGACAAGTATGCCGCCGTCCGCCTCAGCCTTGCGGAAATTTTTGAAGCGAACCGTCGTTGTTACGGCTACCGCAGAC
TACAGGCGTCACTGGCCAGGAAGAGCGTGATCGTCTCGGAAAAGGTTGTCCAGCGCTTGATGAAGCAGGAGCACTTGGCCGTAGCCAGACCGCGCCGACG
GCGTTTCGGATCATACTTGGGAGAAATAAGTCCGGCACCCGAAAACCTGATCAATCGCGACTTTCATGCAGACGCGCCGAACGTGAAATGGCTGACAGAC
ATCACCGAGTTCCAGATCCCAGCTGGCAAGGTCTACCTTTCGCCCATCATCGACTGCTTTGACGGAATGGTCATCAGTTGGTCCATCGGAACCCAACCAG
ATGCGGGTCTCGTCAACACCATGCTGGATGCAGCCATCGGGACCGTCGCCAACGGCGAGGAACGGCCTATCATCCATTCTGATCGAGGAGCCCATTATCG
ATGGCCCGGCTGGTTAACCCGTATCAGCGAAGCAAGACTGGTTCGTTCGATGTCCCGAAAGGGATGCTCGCAAGACAACGCCGCGTGCGAAGGGTTCTTC
GGTCGGTTGAAAACTGAGCTCTTCTATCCACGCGACTGGAAGGCAATCACGATCGAACAGTTCGTCGCCGAGGTGGACGCCTACATCCGATGGTACAATG
AGAAGCGTATCAAGATATCGCTGGGATCACTCAGCCCCGTCGAGTATCGTCAGAGCCTCGGCCTGAAATTATAAAGCAGTCCAACTTTTTATCCGCA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1539 bp512 aa361574+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYSYADRLRAVELYIRLGKRLNTTIRQLGYPTKNALRGWYREYVQHLDLRTQPVARAPKYSEAQRQAALEHFRTHDRCISGTMRALGYPGRGTLTAWVRE
AFPEARTSMVGRSWHPGYSEEVRQAGVIGLCSGDESAQQVADRLGVSRPTLYSWKDQLLGHEASSSMKRRKANPKVPEREELERKLEALQRDVRQLQLEH
DLLKKANELLKKGLGVDLLILSNREKTQLIDALKEVYRLPELLAQLRIARSSYFYHRARMCLADKYAAVRLSLAEIFEANRRCYGYRRLQASLARKSVIV
SEKVVQRLMKQEHLAVARPRRRRFGSYLGEISPAPENLINRDFHADAPNVKWLTDITEFQIPAGKVYLSPIIDCFDGMVISWSIGTQPDAGLVNTMLDAA
IGTVANGEERPIIHSDRGAHYRWPGWLTRISEARLVRSMSRKGCSQDNAACEGFFGRLKTELFYPRDWKAITIEQFVAEVDAYIRWYNEKRIKISLGSLS
PVEYRQSLGLKL

 

Blast result :
Comments
ISAtu3 is 61% aa similar to ISLca1. There is no -1 programmed frameshift.
References
1] Wood,D.W., Setubal,J.C., Kaul,R., Monks,D., Chen,L., Wood,G.E., Chen,Y., Woo,L., Kitajima,J.P., Okura,V.K., Almeida,N.F. Jr., Zhou,Y., Bovee,D. Sr., Chapman,P., Clendenning,J., Deatherage,G., Gillet,W., Grant,C., Guenthner,D., Kutyavin,T., Levy,R., Li,M., McClelland,E., Palmieri,A., Raymond,C., Rouse,G., Saenphimmachak,C., Wu,Z., Gordon,D., Eisen,J.A., Paulsen,I., Karp,P., Romero,P., Zhang,S., Yoo,H., Tao,Y., Biddle,P., Jung,M., Krespan,W., Perry,M., Gordon-Kamm,B., Liao,L., Kim,S., Hendrick,C., Zhao,Z., Dolan,M., Tingey,S.V., Tomb,J., Gordon,M.P., Olson,M.V. and Nester,E.W. (2001) Science 294 (5550), 2317-2323