ISGvi5
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005125 | ND | Gloeobacter violaceus | Gloeobacter violaceus PCC 7421 |
DNA section
IS Length : 1287 bp
Ends
IR Length : 28/37
IRL : TGAGCTTGCCCGGGTTATGTGGACACTCCCTGTAGACTCAATAGCTAAGG
IRR : TGAGCTTGCCCGGCTTTAGTGGACAGTGGGCTGGTGAACGGCTGTCTTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGTGGTTGA | GGT | CCAGGCATGG | 3 |
DNA sequence
TGAGCTTGCCCGGGTTATGTGGACACTCCCTGTAGACTCAATAGCTAAGGAGGAGTCCATGACCGCAAAACCACAACCACAATACACACCCGAACAACGC
GCCGAAGCCGTCCGCATTAGCGAGCAATCCGGCAAGTCCACCTACCAGGTCGCCCGTGACCTCGGCATCTCCCAAACCACCCTCAGCCGCTGGCGACGGC
AGGCCCGCGCCGAGCACAAGCACGCCAACGACCCCGACGCTCCCCTCGGTAGCGACGAACGTCGGGAGTTGACCCGCCTGCGTCGAGAAAACAAACAACT
GCAACTGGAGCGTGACTTTCTAAAAAAAGCGGCTGCTTTCTTCGCCAAAGACCACTCGTAGAACGCTACCGGGCGATGGAAGCACACAAAGACGATTATC
CGGTAGCGTTGATGGCTAGGGTGCTGCAGGTGTCGCGTTCGGGCTACTATGCCTGGCGGCGGCGTGTGCCCTCAAAGCGTCAGTTGCACAACCAGAAACT
TGCTGAGTGCATCGAGGAAGTCTTCACTGCCTCCCGGGCGACTTACGGCAGCCCGCGCGTGCACGCCACGCTGCGCGCGCAGGGGATTGCAGCCGGTCGG
CATCGCGTTGCCAGACTGATGCGTCGGGCGGGTTTGGTGGCTCGGGTGCGCAAGCGCCGCTATCCACGGACCACCGACAGCCGCCATGGGTACCCTGTCG
CGGACAACCTGCTTGCCCGGCAGTTCGGTGCTTGCGAAGCGGACAGCAAATGGGTGGCGGATATCACTTACCTGCCCACCTGCGAAGGTTGGCTGTATCT
GGCGGTGGTGATGGACTTGTTTTCCCGGCGGGTGGTCGGTTGGTCGATGGCGACGCACTTGCGCACTGAATTGGTGCTGACGGCCCTGCAGGCCGCCCTC
GCCAAGCGCGTCCCGGCGTCTTCGGGGCTGTTGTTCCATTCGGACCGCGGCAGCCAATACGCCAGTTGGGCCTACCAGCAGGCGCTCTCAGCGGCGGGTA
TCACCTGCAGCATGAGCCGTAGCGGCACCTGCCTGGACAATGCGGTGGCCGAAAGCTTCTTCGGCACCCTCAAAGTCGAGTTGGTGTATCGGTTGGGTCC
TTTGGACCGCCGGCAGATGCGCACGACGGTGGCCGAGTGGCTGGAGGTATTTTACAACCGGCAACGTCGGCACTCGGCGTTGGGGTACAGGAGCCCAGAC
GAATACGAACGCCACTACTGTAAGGAGGTCAAGCTGAGCAAGACAGCCGTTCACCAGCCCACTGTCCACTAAAGCCGGGCAAGCTCA
GCCGAAGCCGTCCGCATTAGCGAGCAATCCGGCAAGTCCACCTACCAGGTCGCCCGTGACCTCGGCATCTCCCAAACCACCCTCAGCCGCTGGCGACGGC
AGGCCCGCGCCGAGCACAAGCACGCCAACGACCCCGACGCTCCCCTCGGTAGCGACGAACGTCGGGAGTTGACCCGCCTGCGTCGAGAAAACAAACAACT
GCAACTGGAGCGTGACTTTCTAAAAAAAGCGGCTGCTTTCTTCGCCAAAGACCACTCGTAGAACGCTACCGGGCGATGGAAGCACACAAAGACGATTATC
CGGTAGCGTTGATGGCTAGGGTGCTGCAGGTGTCGCGTTCGGGCTACTATGCCTGGCGGCGGCGTGTGCCCTCAAAGCGTCAGTTGCACAACCAGAAACT
TGCTGAGTGCATCGAGGAAGTCTTCACTGCCTCCCGGGCGACTTACGGCAGCCCGCGCGTGCACGCCACGCTGCGCGCGCAGGGGATTGCAGCCGGTCGG
CATCGCGTTGCCAGACTGATGCGTCGGGCGGGTTTGGTGGCTCGGGTGCGCAAGCGCCGCTATCCACGGACCACCGACAGCCGCCATGGGTACCCTGTCG
CGGACAACCTGCTTGCCCGGCAGTTCGGTGCTTGCGAAGCGGACAGCAAATGGGTGGCGGATATCACTTACCTGCCCACCTGCGAAGGTTGGCTGTATCT
GGCGGTGGTGATGGACTTGTTTTCCCGGCGGGTGGTCGGTTGGTCGATGGCGACGCACTTGCGCACTGAATTGGTGCTGACGGCCCTGCAGGCCGCCCTC
GCCAAGCGCGTCCCGGCGTCTTCGGGGCTGTTGTTCCATTCGGACCGCGGCAGCCAATACGCCAGTTGGGCCTACCAGCAGGCGCTCTCAGCGGCGGGTA
TCACCTGCAGCATGAGCCGTAGCGGCACCTGCCTGGACAATGCGGTGGCCGAAAGCTTCTTCGGCACCCTCAAAGTCGAGTTGGTGTATCGGTTGGGTCC
TTTGGACCGCCGGCAGATGCGCACGACGGTGGCCGAGTGGCTGGAGGTATTTTACAACCGGCAACGTCGGCACTCGGCGTTGGGGTACAGGAGCCCAGAC
GAATACGAACGCCACTACTGTAAGGAGGTCAAGCTGAGCAAGACAGCCGTTCACCAGCCCACTGTCCACTAAAGCCGGGCAAGCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 59 | 361 | + | No |
Description : First part of the transposase
ORF sequence :
MTAKPQPQYTPEQRAEAVRISEQSGKSTYQVARDLGISQTTLSRWRRQARAEHKHANDPDAPLGSDERRELTRLRRENKQLQLERDFLKKAAAFFAKDHS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 316 | 1272 | + | No |
Description : Second part of the transposase
ORF sequence :
LSKKSGCFLRQRPLVERYRAMEAHKDDYPVALMARVLQVSRSGYYAWRRRVPSKRQLHNQKLAECIEEVFTASRATYGSPRVHATLRAQGIAAGRHRVAR
LMRRAGLVARVRKRRYPRTTDSRHGYPVADNLLARQFGACEADSKWVADITYLPTCEGWLYLAVVMDLFSRRVVGWSMATHLRTELVLTALQAALAKRVP
ASSGLLFHSDRGSQYASWAYQQALSAAGITCSMSRSGTCLDNAVAESFFGTLKVELVYRLGPLDRRQMRTTVAEWLEVFYNRQRRHSALGYRSPDEYERH
YCKEVKLSKTAVHQPTVH
LMRRAGLVARVRKRRYPRTTDSRHGYPVADNLLARQFGACEADSKWVADITYLPTCEGWLYLAVVMDLFSRRVVGWSMATHLRTELVLTALQAALAKRVP
ASSGLLFHSDRGSQYASWAYQQALSAAGITCSMSRSGTCLDNAVAESFFGTLKVELVYRLGPLDRRQMRTTVAEWLEVFYNRQRRHSALGYRSPDEYERH
YCKEVKLSKTAVHQPTVH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 59 | 1272 | + | Yes |
Chemistry : DDE
ORF sequence :
MTAKPQPQYTPEQRAEAVRISEQSGKSTYQVARDLGISQTTLSRWRRQARAEHKHANDPDAPLGSDERRELTRLRRENKQLQLERDFLKKSGCFLRQRPL
VERYRAMEAHKDDYPVALMARVLQVSRSGYYAWRRRVPSKRQLHNQKLAECIEEVFTASRATYGSPRVHATLRAQGIAAGRHRVARLMRRAGLVARVRKR
RYPRTTDSRHGYPVADNLLARQFGACEADSKWVADITYLPTCEGWLYLAVVMDLFSRRVVGWSMATHLRTELVLTALQAALAKRVPASSGLLFHSDRGSQ
YASWAYQQALSAAGITCSMSRSGTCLDNAVAESFFGTLKVELVYRLGPLDRRQMRTTVAEWLEVFYNRQRRHSALGYRSPDEYERHYCKEVKLSKTAVHQ
PTVH
VERYRAMEAHKDDYPVALMARVLQVSRSGYYAWRRRVPSKRQLHNQKLAECIEEVFTASRATYGSPRVHATLRAQGIAAGRHRVARLMRRAGLVARVRKR
RYPRTTDSRHGYPVADNLLARQFGACEADSKWVADITYLPTCEGWLYLAVVMDLFSRRVVGWSMATHLRTELVLTALQAALAKRVPASSGLLFHSDRGSQ
YASWAYQQALSAAGITCSMSRSGTCLDNAVAESFFGTLKVELVYRLGPLDRRQMRTTVAEWLEVFYNRQRRHSALGYRSPDEYERHYCKEVKLSKTAVHQ
PTVH
Blast result :
Comments
ISGvi5 is 61 % (ORFA) aa similar to ISBph1 and 67% (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Nakamura,Y., Kaneko,T., Sato,S., Mimuro,M., Miyashita,H., Tsuchiya,T., Sasamoto,S., Watanabe,A., Kawashima,K., Kishida,Y., Kiyokawa,C., Kohara,M., Matsumoto,M., Matsuno,A., Nakazaki,N., Shimpo,S., Takeuchi,C., Yamada,M. and Tabata,S. (2003) DNA Res. 10 (4), 181-201.