ISAma1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAOD01000004 | ND | Alteromonas macleodii | Alteromonas macleodii |
DNA section
IS Length : 1261 bp
Ends
IR Length : 29/37
IRL : TGACGTGCTCCAATAGATTCACACACCCAGTTAAGAGATAATTATCTCAA
IRR : TGACGTGGTCAACCAAAATCACACAACCCAGTTAAGCCGCTTTTTGTAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTAGCAATAG | CCA | AGGGCTTATG | 3 |
DNA sequence
TGACGTGCTCCAATAGATTCACACACCCAGTTAAGAGATAATTATCTCAACTGGAGTGTATATGAAAACCCGAAAAAGCTACTCGAAAGAATTCAAACTG
GATGCGGTGAGTCTGGTTACAGAACAAAATTACAAGATTACTGAGGCTGCTCGCAGTCTTGGTATCAGTGCCAATATGTTAGGCCGCTGGCTTAAAGAGC
AAGAATCAGAAGGGGCTCACGCATTTCGTGGCAATGGTAAGTTGACGCCCGAGCAGGAAGAAATTCGCCAACTGCGAGCAGAGAATAAGCGCCTGAAAAT
GGAGAAAGAGATCTTAAAAAAGGCAACGGTCTTCTTTGCCAAAGAAACCAAGTAAAATACGGTTTCATCACCCAACATAAGAAGACCTGGCCGATTAGCC
TGATGTGCGAGATTCTTGGTGTCAGTCGCTGTAATTACTATAGTGCCGTGCGACGCAAGGAACACATTGAACCGGATCCGCAACATGATGAGTTAATTGA
GTGGGTACAGAAAGTAGCAGATGCCAGTGACTTTACCTATGGCGTAAGACGTATGAAACGCGCGTTGAATTCGCTTGGTTATCCGGTTGGAAAACAGCGA
ACGAGAGCCTTGATGAAAGAAGCTGGAGTGAGCGTCAGACGACGTAGAAAATACAAAGTCACGACTAACAGCGACCATCAGCAACCTGTCTTCGATAACG
TGCTGTCACGCGACTTTGATGCTCAAAAGCCAGACCAGGCGTATGTCTCTGACATTACCTACATCTGGACGCAAGAAGGCTGGCTGTATCTGGCCGTAGT
GATTGACTTATTCTCACGAAAAATCGTGGGCTGGAGTATGGCATCGCGGATGAATGCCCAGCTTGTGTGTGATGCCTTAACGATGGCTTTGTGGCAACGT
AAACCTAAAGTGGGCCTGATACATCATTCGGATCGCGGCTCTCAATACGCAAGCCGACAATTTAGACAGTTACTGACGCAATACAAGGTAACAGGTAGCA
TGAGCAGGAAAGGTGATTGTTGGGACAATGCCGTCGTAGAAAGCTTCTTCGGCACATTGAAACAAGAGCGAGTGCAGTGGCGTCACTACCAGAGTCGCCG
TGCTGCCCAGCAGGATATTCTGCAATACATCACTATGTTTTATAACAGTCAGCGAATGCACTCATATCTGGATTACAAGAGTCCAAATCAATATGAGGCT
GACATGGCTAACTTACAAAAAGCGGCTTAACTGGGTTGTGTGATTTTGGTTGACCACGTCA
GATGCGGTGAGTCTGGTTACAGAACAAAATTACAAGATTACTGAGGCTGCTCGCAGTCTTGGTATCAGTGCCAATATGTTAGGCCGCTGGCTTAAAGAGC
AAGAATCAGAAGGGGCTCACGCATTTCGTGGCAATGGTAAGTTGACGCCCGAGCAGGAAGAAATTCGCCAACTGCGAGCAGAGAATAAGCGCCTGAAAAT
GGAGAAAGAGATCTTAAAAAAGGCAACGGTCTTCTTTGCCAAAGAAACCAAGTAAAATACGGTTTCATCACCCAACATAAGAAGACCTGGCCGATTAGCC
TGATGTGCGAGATTCTTGGTGTCAGTCGCTGTAATTACTATAGTGCCGTGCGACGCAAGGAACACATTGAACCGGATCCGCAACATGATGAGTTAATTGA
GTGGGTACAGAAAGTAGCAGATGCCAGTGACTTTACCTATGGCGTAAGACGTATGAAACGCGCGTTGAATTCGCTTGGTTATCCGGTTGGAAAACAGCGA
ACGAGAGCCTTGATGAAAGAAGCTGGAGTGAGCGTCAGACGACGTAGAAAATACAAAGTCACGACTAACAGCGACCATCAGCAACCTGTCTTCGATAACG
TGCTGTCACGCGACTTTGATGCTCAAAAGCCAGACCAGGCGTATGTCTCTGACATTACCTACATCTGGACGCAAGAAGGCTGGCTGTATCTGGCCGTAGT
GATTGACTTATTCTCACGAAAAATCGTGGGCTGGAGTATGGCATCGCGGATGAATGCCCAGCTTGTGTGTGATGCCTTAACGATGGCTTTGTGGCAACGT
AAACCTAAAGTGGGCCTGATACATCATTCGGATCGCGGCTCTCAATACGCAAGCCGACAATTTAGACAGTTACTGACGCAATACAAGGTAACAGGTAGCA
TGAGCAGGAAAGGTGATTGTTGGGACAATGCCGTCGTAGAAAGCTTCTTCGGCACATTGAAACAAGAGCGAGTGCAGTGGCGTCACTACCAGAGTCGCCG
TGCTGCCCAGCAGGATATTCTGCAATACATCACTATGTTTTATAACAGTCAGCGAATGCACTCATATCTGGATTACAAGAGTCCAAATCAATATGAGGCT
GACATGGCTAACTTACAAAAAGCGGCTTAACTGGGTTGTGTGATTTTGGTTGACCACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 62 | 355 | + | No |
Description : First part of the transposase
ORF sequence :
MKTRKSYSKEFKLDAVSLVTEQNYKITEAARSLGISANMLGRWLKEQESEGAHAFRGNGKLTPEQEEIRQLRAENKRLKMEKEILKKATVFFAKETK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 942 bp | 313 aa | 289 | 1230 | + | No |
Description : Second part of the transposase
ORF sequence :
APENGERDLKKGNGLLCQRNQVKYGFITQHKKTWPISLMCEILGVSRCNYYSAVRRKEHIEPDPQHDELIEWVQKVADASDFTYGVRRMKRALNSLGYPV
GKQRTRALMKEAGVSVRRRRKYKVTTNSDHQQPVFDNVLSRDFDAQKPDQAYVSDITYIWTQEGWLYLAVVIDLFSRKIVGWSMASRMNAQLVCDALTMA
LWQRKPKVGLIHHSDRGSQYASRQFRQLLTQYKVTGSMSRKGDCWDNAVVESFFGTLKQERVQWRHYQSRRAAQQDILQYITMFYNSQRMHSYLDYKSPN
QYEADMANLQKAA
GKQRTRALMKEAGVSVRRRRKYKVTTNSDHQQPVFDNVLSRDFDAQKPDQAYVSDITYIWTQEGWLYLAVVIDLFSRKIVGWSMASRMNAQLVCDALTMA
LWQRKPKVGLIHHSDRGSQYASRQFRQLLTQYKVTGSMSRKGDCWDNAVVESFFGTLKQERVQWRHYQSRRAAQQDILQYITMFYNSQRMHSYLDYKSPN
QYEADMANLQKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 389 aa | 62 | 1230 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTRKSYSKEFKLDAVSLVTEQNYKITEAARSLGISANMLGRWLKEQESEGAHAFRGNGKLTPEQEEIRQLRAENKRLKMEKEILKKGNGLLCQRNQVKY
GFITQHKKTWPISLMCEILGVSRCNYYSAVRRKEHIEPDPQHDELIEWVQKVADASDFTYGVRRMKRALNSLGYPVGKQRTRALMKEAGVSVRRRRKYKV
TTNSDHQQPVFDNVLSRDFDAQKPDQAYVSDITYIWTQEGWLYLAVVIDLFSRKIVGWSMASRMNAQLVCDALTMALWQRKPKVGLIHHSDRGSQYASRQ
FRQLLTQYKVTGSMSRKGDCWDNAVVESFFGTLKQERVQWRHYQSRRAAQQDILQYITMFYNSQRMHSYLDYKSPNQYEADMANLQKAA
GFITQHKKTWPISLMCEILGVSRCNYYSAVRRKEHIEPDPQHDELIEWVQKVADASDFTYGVRRMKRALNSLGYPVGKQRTRALMKEAGVSVRRRRKYKV
TTNSDHQQPVFDNVLSRDFDAQKPDQAYVSDITYIWTQEGWLYLAVVIDLFSRKIVGWSMASRMNAQLVCDALTMALWQRKPKVGLIHHSDRGSQYASRQ
FRQLLTQYKVTGSMSRKGDCWDNAVVESFFGTLKQERVQWRHYQSRRAAQQDILQYITMFYNSQRMHSYLDYKSPNQYEADMANLQKAA
Blast result :
Comments
ISAma1 is 68% (ORFA) aa similar to ISSod1 and 64% (ORFB) aa similar to ISRso8.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Rodriguez-Valera,F., Ferriera,S., Johnson,J., Kravitz,S.,Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.