ISAzvi9
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001157 | ND | Azotobacter vinelandii | Azotobacter vinelandii AvOP |
DNA section
IS Length : 1246 bp
Ends
IR Length : 30/37
IRL : TGATCTTGCCCCGCCTCAGTGGACACCCCGTTAAGCGATACACTGCAACG
IRR : TGATCTTGCCCAGCCCTCGTGGACACAGGTTTAAGCACGCATCCGGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGGGAACGC | CCCGCCGGTA | 0 |
DNA sequence
TGATCTTGCCCCGCCTCAGTGGACACCCCGTTAAGCGATACACTGCAACGAGGTGATCCATGGCAAGGCAAGCAAGCCCGATGAAACCAACCCGTATCCG
CCACTCCCAAGCCTACAAGGACGAAGCGCTGGCCTTGGCCGAACGGATTGGCGTCAGCAAAGCGGCCGAACAGCTTGGGCTGCCCCCCTCTCAGCTCTAT
GGCTGGCGGAGCAGGAAACAGCAGGCCCAGACCAGCAGCGAGCGCGAGCAGTTGTTGGCCGACGAAAACGCCCGCCTCAAACGGCTGTTGGCCGAACAGT
CCGAGGAGTTGGCCATCGTAAAAAAAGCCGCCGCGTACTTTGCCAGGAGCCTCAAGTGAAGTACGCCTTCATGCAAGCCCATACTCGGCAGTTTCCACTG
AAGGTCATGTGCCGTGTGCTGGGCATTGCCCGCAGCGGCTACTACGCCTGGTGTTCGCGCACGCTCTCGAAGCGCCGCCAGCAACAGACCCAACTGGACC
GCCTGGTAGCTCAGGCCTACCACGAGCGGAAGGGGCGCAGCGGGGCTCCGCGGTTATGCCTCGACCTGAAGGCGTCGGGCCTGCCCTGCAATCGCAAGAC
CGTGGCTGCTTCGATGCGACGCCAGGGACTGCGGGCCAGGGCTGCCAGGAAGTTCAAGGCCACCACGAATTCCAGGCACTCACTACCGGTGGCGGACAAC
CTGCTCCGGCAGGACTTCACGGCAGCGGCGCCCAACCGGAAGTGGGTGGGCGACATGACTTACCTGCGTACGGAAGAAGGCTGGTTGTATCTGGCCGTGA
TCATCGACCTGTACTCGCGCAGGGTGATCGGCTGGGCCATGAACGAGCGCATGACGGCCGAGCTGGCCTGCGATGCCCTGCGCATGGCCCTGTGGCGGCG
CAAGCAGCCTCAAGGCGTGATCGTGCATACGGATCGAGGCAGCCAGTACTGTTCGGCGGCCTATCGGGAACTGCTTCGTGCCCATCGGCTGCGCGGCAGC
ATGAGCGCGAAGGGCAATTGCTACGACAACGCCTGTGCCGAAAGCTTCTTTCACAGTCTGAAGGTCGAGTGCATCCATGGGGAACGCTTCGGTACGCATG
CCCAGATGCGTCAAACCGTGTTCGAATACATCGAAACCGACTACAACCGCCAGCGTCGTCACAGCACACTGGGATACATCAGTCCGGAAGCCTTCGAGGC
CCGGATGCGTGCTTAAACCTGTGTCCACGAGGGCTGGGCAAGATCA
CCACTCCCAAGCCTACAAGGACGAAGCGCTGGCCTTGGCCGAACGGATTGGCGTCAGCAAAGCGGCCGAACAGCTTGGGCTGCCCCCCTCTCAGCTCTAT
GGCTGGCGGAGCAGGAAACAGCAGGCCCAGACCAGCAGCGAGCGCGAGCAGTTGTTGGCCGACGAAAACGCCCGCCTCAAACGGCTGTTGGCCGAACAGT
CCGAGGAGTTGGCCATCGTAAAAAAAGCCGCCGCGTACTTTGCCAGGAGCCTCAAGTGAAGTACGCCTTCATGCAAGCCCATACTCGGCAGTTTCCACTG
AAGGTCATGTGCCGTGTGCTGGGCATTGCCCGCAGCGGCTACTACGCCTGGTGTTCGCGCACGCTCTCGAAGCGCCGCCAGCAACAGACCCAACTGGACC
GCCTGGTAGCTCAGGCCTACCACGAGCGGAAGGGGCGCAGCGGGGCTCCGCGGTTATGCCTCGACCTGAAGGCGTCGGGCCTGCCCTGCAATCGCAAGAC
CGTGGCTGCTTCGATGCGACGCCAGGGACTGCGGGCCAGGGCTGCCAGGAAGTTCAAGGCCACCACGAATTCCAGGCACTCACTACCGGTGGCGGACAAC
CTGCTCCGGCAGGACTTCACGGCAGCGGCGCCCAACCGGAAGTGGGTGGGCGACATGACTTACCTGCGTACGGAAGAAGGCTGGTTGTATCTGGCCGTGA
TCATCGACCTGTACTCGCGCAGGGTGATCGGCTGGGCCATGAACGAGCGCATGACGGCCGAGCTGGCCTGCGATGCCCTGCGCATGGCCCTGTGGCGGCG
CAAGCAGCCTCAAGGCGTGATCGTGCATACGGATCGAGGCAGCCAGTACTGTTCGGCGGCCTATCGGGAACTGCTTCGTGCCCATCGGCTGCGCGGCAGC
ATGAGCGCGAAGGGCAATTGCTACGACAACGCCTGTGCCGAAAGCTTCTTTCACAGTCTGAAGGTCGAGTGCATCCATGGGGAACGCTTCGGTACGCATG
CCCAGATGCGTCAAACCGTGTTCGAATACATCGAAACCGACTACAACCGCCAGCGTCGTCACAGCACACTGGGATACATCAGTCCGGAAGCCTTCGAGGC
CCGGATGCGTGCTTAAACCTGTGTCCACGAGGGCTGGGCAAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 60 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MARQASPMKPTRIRHSQAYKDEALALAERIGVSKAAEQLGLPPSQLYGWRSRKQQAQTSSEREQLLADENARLKRLLAEQSEELAIVKKAAAYFARSLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1182 bp | 393 aa | 35 | 1216 | + | No |
Description : Second part of the transposase
ORF sequence :
AIHCNEVIHGKASKPDETNPYPPLPSLQGRSAGLGRTDWRQQSGRTAWAAPLSALWLAEQETAGPDQQRARAVVGRRKRPPQTAVGRTVRGVGHRKKSRR
VLCQEPQVKYAFMQAHTRQFPLKVMCRVLGIARSGYYAWCSRTLSKRRQQQTQLDRLVAQAYHERKGRSGAPRLCLDLKASGLPCNRKTVAASMRRQGLR
ARAARKFKATTNSRHSLPVADNLLRQDFTAAAPNRKWVGDMTYLRTEEGWLYLAVIIDLYSRRVIGWAMNERMTAELACDALRMALWRRKQPQGVIVHTD
RGSQYCSAAYRELLRAHRLRGSMSAKGNCYDNACAESFFHSLKVECIHGERFGTHAQMRQTVFEYIETDYNRQRRHSTLGYISPEAFEARMRA
VLCQEPQVKYAFMQAHTRQFPLKVMCRVLGIARSGYYAWCSRTLSKRRQQQTQLDRLVAQAYHERKGRSGAPRLCLDLKASGLPCNRKTVAASMRRQGLR
ARAARKFKATTNSRHSLPVADNLLRQDFTAAAPNRKWVGDMTYLRTEEGWLYLAVIIDLYSRRVIGWAMNERMTAELACDALRMALWRRKQPQGVIVHTD
RGSQYCSAAYRELLRAHRLRGSMSAKGNCYDNACAESFFHSLKVECIHGERFGTHAQMRQTVFEYIETDYNRQRRHSTLGYISPEAFEARMRA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1157 bp | 385 aa | 60 | 1216 | + | Yes |
Chemistry : DDE
ORF sequence :
MARQASPMKPTRIRHSQAYKDEALALAERIGVSKAAEQLGLPPSQLYGWRSRKQQAQTSSEREQLLADENARLKRLLAEQSEELAIVKKSRRVLCQEPQV
KYAFMQAHTRQFPLKVMCRVLGIARSGYYAWCSRTLSKRRQQQTQLDRLVAQAYHERKGRSGAPRLCLDLKASGLPCNRKTVAASMRRQGLRARAARKFK
ATTNSRHSLPVADNLLRQDFTAAAPNRKWVGDMTYLRTEEGWLYLAVIIDLYSRRVIGWAMNERMTAELACDALRMALWRRKQPQGVIVHTDRGSQYCSA
AYRELLRAHRLRGSMSAKGNCYDNACAESFFHSLKVECIHGERFGTHAQMRQTVFEYIETDYNRQRRHSTLGYISPEAFEARMRA
KYAFMQAHTRQFPLKVMCRVLGIARSGYYAWCSRTLSKRRQQQTQLDRLVAQAYHERKGRSGAPRLCLDLKASGLPCNRKTVAASMRRQGLRARAARKFK
ATTNSRHSLPVADNLLRQDFTAAAPNRKWVGDMTYLRTEEGWLYLAVIIDLYSRRVIGWAMNERMTAELACDALRMALWRRKQPQGVIVHTDRGSQYCSA
AYRELLRAHRLRGSMSAKGNCYDNACAESFFHSLKVECIHGERFGTHAQMRQTVFEYIETDYNRQRRHSTLGYISPEAFEARMRA
Blast result :
Comments
ISAzvi9 is 90% (ORFA) and 84% (ORFB) aa similar to ISPa20.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank.