ISfinder Mirror Site

ISAmas1

Family IS256
Group IS1249

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_JH992955	ND	Actinobaculum massiliae	Actinobaculum massiliae ACS-171-V-Col2

DNA section

IS Length : 1411 bp

Ends

IR Length : 24

IRL : GGGTTAAGTAGCAAAATGTGTGTCTGATTTTCGCAGTCCTCACTGCTCAG
IRR : GGGTTAAGTAGCAAAATGTGTGTCCGGTCGGCGTGTCGTCGGTCGGACAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCCCACCTGC		CTTTTTGAC	0

DNA sequence

GGGTTAAGTAGCAAAATGTGTGTCTGATTTTCGCAGTCCTCACTGCTCAGACGGCATAAATCGGCCGTATATAAGGTTTCGCACCTTATATATGCTCGAT
TCTCGTTCAATCCACCGGAATGGCATCCTCATGTGCTAGTCGGGGATTGGTGTGCTTTCTGGGTGAGTAGAAACAGACCACGTTGCCCAGTATGCGCTGG
GGAAATGAAGAAAAATGGCACCACTTCCAAAGGCACAACCAGGTGGCGGTGCAAAAACCCAGATTGCGGCAGCTCGACGACACGCCGCCGCACCGATCAC
ACCCAAACTCGCGACTTTCAAGCGTTCATCTCTTATGCGACTTGCACCGCCTCATTGGCCGAGGTCGCTCGCCTGCGAAACATCAGCCGGTGGACCCTAG
ATCGCCGATTCGCGCCGTTTTGGCTCATCGATGTGCCCAACGATGGTGAACCTCACCGCGTCTATGACCAGATCTTCATCGACGGTACCTACACCGCCGC
TGGCTGCCTCCTCGTCGCGGCCAGCTATGACCACGTCATCGCATGGCACTGGGCGAAAAGCGAAACCGCCCACGCCTACACCCAATTGCTCAAAAAGATC
GCCGAGCCACTATGTGTGGTCCTCGACGGCGGACAAGGAGCACTCACCGCCATTAAAGCGTGCTGGCCAAACACGCTCATTCAGCGCTGCCTAGTCCATG
CCCAACGCGCGATCCGTCGCTACACGTCGACCCAACCACGCACCGACGCGGGCAAGGCGATCTACGCACTGGCGTTGAAACTGACCCGCATCACCACACT
TGACCAGGCACGCGAATGGACACTGCGTCTCCACGACTTCGGACAGGTCTATAAAGCATTCCTCGACGAAAAGACCCCCGTGCCAAAAGAACGGCGCACG
CTGAATCACCAGTGGGAATGGACCCACCTGCGAGTTCGCAAGGCATACAACTCCCTGCTGCACCTCTCACGGAAAGGATGGCTGTTTACCTACCTGCAGC
CGCCCAAAAACGCACTCGAGCCAAACCGGTGGGTCTCAACAACCAACAGCCTCGAAGGCGGCATCAACGCCCAGCTCAAACGCATCGCCGATGCACACCG
CGGCAGGTCAGGAGAACGCCAACGAAAAATGCTCGAGTGGTGCCTGTATGCAAAAACGCAACTGCCTGACGATCCACTCACGATCGCCAGGCAGTGCAAT
TTCGGACAAGACCAACTCGCCAAAGTCAACGATCTTGCCGAAGAAGACCACAACAAAGCCGACCAAGAAACAGGACGACCAGCCTTCTACGACAACGCTA
TCCCAACCGAATACAACCACTCGATACGAATCAGAAAAGGCCCCATGAAATAGCACAAAAAGTGTCCGACCGACGACACGCCGACCGGACACACATTTTG
CTACTTAACCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1149 bp	382 aa	205	1353	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKNGTTSKGTTRWRCKNPDCGSSTTRRRTDHTQTRDFQAFISYATCTASLAEVARLRNISRWTLDRRFAPFWLIDVPNDGEPHRVYDQIFIDGTYTAAG
CLLVAASYDHVIAWHWAKSETAHAYTQLLKKIAEPLCVVLDGGQGALTAIKACWPNTLIQRCLVHAQRAIRRYTSTQPRTDAGKAIYALALKLTRITTLD
QAREWTLRLHDFGQVYKAFLDEKTPVPKERRTLNHQWEWTHLRVRKAYNSLLHLSRKGWLFTYLQPPKNALEPNRWVSTTNSLEGGINAQLKRIADAHRG
RSGERQRKMLEWCLYAKTQLPDDPLTIARQCNFGQDQLAKVNDLAEEDHNKADQETGRPAFYDNAIPTEYNHSIRIRKGPMK

Blast result :

Comments

ISAmas1 is 74% aa similar to IS3507.

References

1] ISfinder annotation (2013)
2] Earl,A., Ward,D., Feldgarden,M., Gevers,D., Saerens,B.,
Vaneechoutte,M., Walker,B., Young,S.K., Zeng,Q., Gargeya,S.,
Fitzgerald,M., Haas,B., Abouelleil,A., Alvarado,L., Arachchi,H.M.,
Berlin,A., Chapman,S.B., Goldberg,J., Griggs,A., Gujja,S.,
Hansen,M., Howarth,C., Imamovic,A., Larimer,J., McCowen,C.,
Montmayeur,A., Murphy,C., Neiman,D., Pearson,M., Priest,M.,
Roberts,A., Saif,S., Shea,T., Sisk,P., Sykes,S., Wortman,J.,
Nusbaum,C. and Birren,B. (2012) Direct submission GenBank.