ISfinder Mirror Site

ISBvi1

Family IS4
Group IS4

MGE type ISRelated element(s) :
Isoform Synonym(s) ISRme8

Accession number	Transposition	Origin	Host
	ND	Burkholderia vietnamiensis	Burkholderia vietnamiensis G4 Ralstonia metallidurans CH34

DNA section

IS Length : 1455 bp

Ends

IR Length : 15/16

IRL : CAATACTGTTCAGATAGTATTTTTAAAGCCATAATCCTCATCCATCGAAG
IRR : AAATACTGTTCAGATACGAAGCACCGGCACTGGCTGATGCCGCAAATGGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGCGGGTCC	GGTCTAGTTG	GCGTTTGCAG	10

DNA sequence

CAATACTGTTCAGATAGTATTTTTAAAGCCATAATCCTCATCCATCGAAGCTTCAATGGATGAGGGTGATGGCCAGAACACGCAAGACGCTGCCGTCGAG
AGTTGACGTTGCGCATTTGATCAGCGCCGGGGTGCTGGCCAGCGTGTGTCCACGGACGTTGATCGAAGAAGTGTTGGCCGAGACCGGCAAGGCCAGCCAG
CGGGAGCGGCTGCTGCCCGCGCCGGCAGTGGTGTATTACGTGATGGCGCTGGCGCTGTGGCGCGAAGCGCCGCTGGAGGAAGTGCTGCGCGTGGTGTGCG
AAGGCCTGCAATGGCTCGGCGGCGGCCACACTGAGGCGGTGCAGGCCAGCAAGTCCGCGATCTCGCAGGCGCGCAGCCGGCTGGGCCCCGAGGTCATGCG
CCAATTGGCCGATCGGGTGCTGCGCCCGCTGGCCGCGCCGGGGGCGCCAGGGGCGTGGTACCGGGGATTGCGCGTGATGGCCCTGGACGGCAGCTGCATG
GACGTGGCCGACGAGGCGGCCAACGCCAAGTTCTTCGGCTACCCGGGCGCCTCGCGCGGCCAAAGCGCCTTCCCGCAGGCGCGCGTTCTGGGCCTGGTGG
AATGCGGCACGCACGCGGTGGTGGCAGCCGGCATCGCACCCTACGGCCACAGCGAGCAGGTCATGGCCGCCCAGTTGCTGCCCGCCAAGCTCACGCCCGA
GATGCTGGTGCTGGCCGACCGCAACTTCTACGGCTTCAAGCTCTGGCAGACCGCCTGCGCTACGGGCGCCAAGCTGGCCTGGCGGGTCAAATCCAACCTC
AAGCTGCCGGTAGAGCAGATGTTGCCCGATGGCTCGTACCTGAGCCGCGTGTTCGACAGCGACGACCGCGCGCGGCGCGCAGGGCAAACGGTGCGTGTGA
TCGACTACGCGCTCGAGGGCTCGGCCACGCCGGCGCAGGGCAGCTACCGGCTGCTGACCAACCTCCTGGACCCCGATGCGGCGCCGGCCCTGGAGCTGGC
CGCGCTGTACCACGAGCGCTGGGAGATCGAGGGGGTGTTCGATGAGTTCAAGACGCATTTGCGCGCCAACAGCACGGTGCTGCGCAGCAAGACGCCCGAG
CTGGTGCAGCAGGAGCTGTGGGGGCTGCTGCTGGCGCACTTTGCGATTCGCCAGTTGATGGCGCAGGCGGCCTGGGCGCGCGAACTCGATCCGGATCGCC
TGAGCTTCATGCACGCCGTGCGCGTGATCAAGCGCAAGATGCCGCAAGCTGCGGCCGTTTCCCCCTGAGCGTTTGCCTGCCTGGCGCGAGGCCCTGCTGG
CCGAGATCGCGCGCGGGCGCTGCGTGAGCAGTCGTGGGCGCCTGAACCCGCGCGGCGTCAAGCGCAAGATGAGCAACTTCAATGTTCGGCATCGCGGCGC
AGCGCTCCATTTGCGGCATCAGCCAGTGCCGGTGCTTCGTATCTGAACAGTATTT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1206 bp	402 aa	60	1265	+	No

ORF function : Transposase
Description :

ORF sequence :

MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYYVMALALWREAPLEEVLRVVCEGLQWLGGGHTEAVQASKSA
ISQARSRLGPEVMRQLADRVLRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFGYPGASRGQSAFPQARVLGLVECGTHAVVAAGIAPYGHSEQ
VMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLAWRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLT
NLLDPDAAPALELAALYHERWEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAHFAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAV
SP

Blast result :

Comments

ISBvi2 is 46% aa similar to IS1271.
ISBvi2 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-103-E(C1).
Genome in progress : Accession number : NZ_AAEH00000000
Other Accession number : NC_007974

References

[1]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
[2]Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2006)Unpublished