ISSav4

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003155 ND Streptomyces avermitilis
Streptomyces avermitilis MA-4680
DNA section
IS Length : 1426 bp

Ends


IR Length : 16

IRL : TAGGGCTCCGTTAGGTCTGTCTCGCGGTCCTGTTTGGGGGCATGTTGAAG
IRR : TAGGGCTCCGTTAGGTTGCTCTGGCTCTTGGGTTGTGGCTGGTGGTGGAT

Insertion site


Left flankDirect repeatRight flankDR Length
CGCGGCGCACCTAGGTGTATGAGG4
ACTCAATCATCTAGGGGCTGCGCG4
GAACCCAGCACTAGTACCCGCGGA4

DNA sequence

TAGGGCTCCGTTAGGTCTGTCTCGCGGTCCTGTTTGGGGGCATGTTGAAGGTGTGGATGCACATGAAGTGAACCGTGCTCGGGCGACGTTGGCATTATTC
GTGGCTGACGTGTTCGCGTCGGTGCCGCGTAAGGACCAGCGGGCGAAGGGCGACTGCTATCTGCGGGGGCTGATGCTGGACGGACGGCGTAAGTCGATCC
AGCCGATGGCAGAGCGGCTGCCGGATGGCAACGAGCAGAACCTGCAGCAGTTCGTGAACCAGTCGACCTGGGATCCGGTGCCGGTGCGGCGGCGGATCGC
GGAGCGGATGGTGGCGCAGATCGGCCCGGATGCCTGGGCGGTCGATGACGTGTCGTTCCCCAAGGACGGCAAGATGTCGGTCGCGGTCGCGCACCAGTAC
TGCGGGGCGCTGGGCAAGCAGGCCAACTGCCAGGTCGCGGTGAGCGTGCACGCGGTCTCCGACACTGCCTCCTGCCCGCTGCAGTGGCGGTTGTTCGTGC
CCCAGGAGTGGGCGCACGACGCGCCACGGCGGCAGAAGACCGGGATACCGCCAGAGGTCGGGCACCGGGAGAAGTGGCGCCTGGCTCTGGACATCTTCGA
CGAGCTGGCCGGGTGGGGGCTGGTGCCGCCGGTGGTGGTGGCCGACGCCGGCTACGGGCAGAACGCCGACTTCCGCGACGGCCTGGACGGCCGGGGCATC
GGCTATGTCGCGGCCATCCGCTCGGATGTGACCGTCCATCCGCACGATGCCGAGCCTTTCGCGCCGCCCTGGTCGGGCAACGGCCGCAAGCCCCAGCCCC
GCTACCGCGACAAGCCGTCCTCGGTGGCGGCGCTGGCGACCGGCCACGGGCGGCAGGCCTTCACCGAGGTGACCTGGCGGGAAGGCTCCCGCGGACCGAT
GCGCTCGCATTTCCTGTCCCTGCGGGTGCGGCCGGCCGGGGTCAAATCCCGACGCCTCGCCCAGGCCGCCGCCACAGACGAAAACTGCTGGGACGGTGTC
CTGCCCGAGGTCACACTGCTGGTCGAATGGCCCGAAGGCGCCGAGGCGCCCACCGACTACTGGCTGTCCAACCTGCCCGACACGCTCTCCCTCGCCGAAC
TGGTCCGCCTGGCGAAGATCCGCTGGCGCATCGAGCACGACTACCGCGAACTCAAGCACGGCCTCGGCCTGGACCACTTCGAGGGCCGCTCCTGGACCGG
CTGGCACCACCACGTCTCCCTGGTCACCGCCGCCCACGCGTTCCTCACCGAACAGCGTCTGGCCCCAAAAGCCGGTACAGCGGACTCACCCTCTACCAGA
CCCTCGACGCCATCCAGGACCTGCTGAACTGCTGGAGCGGCACCTGCACCACCTGCCACCGCCCCCTGCCCAAAACATCCACCACCAGCCACAACCCAAG
AGCCAGAGCAACCTAACGGAGCCCTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1272 bp424 aa531324+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDAHEVNRARATLALFVADVFASVPRKDQRAKGDCYLRGLMLDGRRKSIQPMAERLPDGNEQNLQQFVNQSTWDPVPVRRRIAERMVAQIGPDAWAVDDV
SFPKDGKMSVAVAHQYCGALGKQANCQVAVSVHAVSDTASCPLQWRLFVPQEWAHDAPRRQKTGIPPEVGHREKWRLALDIFDELAGWGLVPPVVVADAG
YGQNADFRDGLDGRGIGYVAAIRSDVTVHPHDAEPFAPPWSGNGRKPQPRYRDKPSSVAALATGHGRQAFTEVTWREGSRGPMRSHFLSLRVRPAGVKSR
RLAQAAATDENCWDGVLPEVTLLVEWPEGAEAPTDYWLSNLPDTLSLAELVRLAKIRWRIEHDYRELKHGLGLDHFEGRSWTGWHHHVSLVTAAHAFLTE
QRLAPKAGTADSPSTRPSTPSRTC

 

Blast result :
Comments
ISSav4 is 54% aa similar to ISRso17. ISSav4 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-162-E(C1). The copy number in the Streptomyces avermitilis MA-4680 chromosome is 3.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H., Shiba,T., Sakaki,Y., Hattori,M. and Omura,S. (2003) Nat. Biotechnol. 21 (5), 526-531