ISfinder Mirror Site

ISSav3

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_003155	ND	Streptomyces avermitilis	Streptomyces avermitilis MA-4680

DNA section

IS Length : 1400 bp

Ends

IR Length : 17

IRL : TACTGCAACGGTGCTTGCCGTGACTGGTGGCCAGTTCAGTCGTTGGTCTG
IRR : TACTGCAACGGTGCTTGTTCTGAGTGTTGGGCAGGTTTCAGGGGCTGTGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAAACGACGT	CTAN	TGTCGAACGC	4

DNA sequence

TACTGCAACGGTGCTTGCCGTGACTGGTGGCCAGTTCAGTCGTTGGTCTGGTTATGGGTGGGGACCTTGCTGGTGTCAGGTTGTGGGCGGGTGAACTCGA
GGCGGTGCACGAGCGGTTCGTGCACCGGTTCAGCCGGGAAGAGCCGCGTCAGTCGGCGCTGGCCTATATGCGGGGGCTGGTCGCGCCGCTGGAGCGGAAG
AACGGCTGGACGCTGGCTGAGGAGGCCGGGCATGCCGGTCCCGATCGCATCCACCGGTTGCTGAACCGGATCGAGTGGGACGCCGACGAGGTCCTGGACG
ACGTGCGTGACTACGTCGTCGAGAACCTCGGCGACCGGGATGCCGTCCTGATCGTCGACGACACCGGATTCCTGAAGAAGGGTGTCCGTTCGGCCGGGGT
CCAGCGGCAGTACTCCGGCACCGCGGGACGCACGGAGAACTCCCAGATCGGGGTCTTTCTCGCGTATGCAACGGGCCGCGGACGCACGCTGATCGACCGG
CGGCTGTATCTGCCCACGTCCTGGACGGATGACCGCGAGCGATGCCGACGGGCAGGCATCGACGACAGTGTCGCCTTCGAGACGAAGGTGGCCATGGCCA
AGGCGATGGTCCGCCGGGCCATCGCCGACAGGATCCCGTTCGGCTGGGTGACGGCGGACGCCGCCTATGGCTTCAGCAAGGGCTGGCGGTTCGAGCTGGA
GCAGGCGGACGTCTTCCATGTCATGGCCACCACCCGGCACGACACCGTCGTCACCCGCTGGTCCATCGACCACCCCGTCCACGACCTGTTCCCCGGCCTG
CCCCGGCAGAAATGGAAACGCCGTTCCTGCGGCGAGGGAGCCCACGGCCGGCGGATCTACGACTGGGCCCGCGTCGAGGTGAGGCCCTGGCACCGCGAGG
ACCGCCGGCACTGGGTTCTCGCCCGCCGCAGCGTGAGCAGGCCCGAGGAGATCTCCTACTACATCGCCTACTGTCCCGCCGGCACCACGCTGGACGAGCT
GATCCGCGTCGCGGGCAGCCGATGGGCCGTCGAGGAATGCTTCCAGACGGCGAAGCAGGAATGCGGTCTGGACGACTACCAGGTCCGCCGCTACCCCGGC
TGGCACCGCCACATGACCCTGGCCATGGCCGCTCATGCCTGCCTGACCGTCCTGCGAGCCCGTGAACTGGACATCGGGAAAGCAGAAACGGATCCTCCCA
GCTCATCCACTACAGCCTCGCCGAGATCCGACGACTGATCACCCGCCTCACCGACCGCCGCCCCACCCCGATCGACCACATCCTGCATTGGTCGACATGG
CGCCGCCGACGCCAACACCAAGCCCGCATCAGTCACTACAAACGACGCGGACACAGCCCCTGAAACCTGCCCAACACTCAGAACAAGCACCGTTGCAGTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1182 bp	394 aa	54	1235	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGGDLAGVRLWAGELEAVHERFVHRFSREEPRQSALAYMRGLVAPLERKNGWTLAEEAGHAGPDRIHRLLNRIEWDADEVLDDVRDYVVENLGDRDAVLI
VDDTGFLKKGVRSAGVQRQYSGTAGRTENSQIGVFLAYATGRGRTLIDRRLYLPTSWTDDRERCRRAGIDDSVAFETKVAMAKAMVRRAIADRIPFGWVT
ADAAYGFSKGWRFELEQADVFHVMATTRHDTVVTRWSIDHPVHDLFPGLPRQKWKRRSCGEGAHGRRIYDWARVEVRPWHREDRRHWVLARRSVSRPEEI
SYYIAYCPAGTTLDELIRVAGSRWAVEECFQTAKQECGLDDYQVRRYPGWHRHMTLAMAAHACLTVLRARELDIGKAETDPPSSSTTASPRSDD

Blast result :

Comments

ISSav3 is 57% aa similar to IS1452. ISSav3 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-124-E(C1). The copy number in the Streptomyces avermitilis MA-4680 chromosome is 1.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H., Shiba,T., Sakaki,Y., Hattori,M. and Omura,S. (2003) Nat. Biotechnol. 21 (5), 526-531