ISTth2
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005838 | ND | Thermus thermophilus | Thermus thermophilus HB27 |
DNA section
IS Length : 1184 bp
Ends
IR Length : 17/18
IRL : CTGAGTTCTCATCACTTTGAGGGCTGGGGCTTGACAAGGGAAGAGAAGAG
IRR : CTGAGCTCTCATCACTTTCATCACTTTCCTCCCCCCAGTTCCTGAAGCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGGCCTTAA | GGCCGCCCC | CGCTTGACGT | 9 |
| CCCCCCGTCA | AGAGGGGGG | GCCCTTGACA | 9 |
| CCGCTAAAGG | GTCCTCCAG | GGCTGGGCTT | 9 |
DNA sequence
CTGAGTTCTCATCACTTTGAGGGCTGGGGCTTGACAAGGGAAGAGAAGAGGGGGGGTAAGTAAGGTGTGCCGCCCACCACCCCCCTTTCCCAAGTTATCA
CCCTCTGGGTCCATAAGGCCTTCGCCTCCCTCAGGAAAACCATTCGCTCCAACCTCGCCCTCTTCCTCTCCACCCTCCTCACCGCTCCCCTGGACCCCAC
CCTCTCCGACCTCGCCCGCAGAACCCCCCTCCCCACCCTGGCCCAAAGCCGCCTCAATCGCCTCTGGCGCTTCCTCCATCACCCCACCCTGCAAAACCCC
TGGGCCCTCACCGAAGCCCTCCTCCCCCTCCTCGCCCGTCGTTTCCCCAAAGACCGCCCCCTCCCCCTCATCGTGGACTGGACCTTCGCGGAGGACGGCA
GGCACCAAGCCCTGGTGGCCGCCCTTCCCCTCAAGGGAAGGGCCCTGGTGGTGGCCTTCGCTCTTCACCCCCTCTCCCCTTTCCCCAGTCAAAACCGGGT
GGAGGAGGAGTTCCTCCACCGCCTGGGCCGCGCCGTCCAGGACCTGGGATATACCCCCCTCTTCCTCCTGGACCGCGGCTTTGACCGGGTCTCCCTGATG
CGAAAGCTCCAGGGGTGGGGCATGGGCTTCCTCATCCGCCTGCGGCAGAACCGGGAGGTGGAACCCCGAGGGGGGAAGCGCCTTCCCCTGAAGGAGGGCT
ACCGGCGTGTGGTCCACCCCCTGCGGGAGGAGGTCCGCCTTTTCGGACACGGTGGGGAGGAGGTAGAGGTCACCCTCCTGGTGTACCCGGGGGGTCGGGA
GCCCTGGTATCTGGCCTATTCGGGCCCTTTTGGAGGGAAGCCGCCCTACGGGTGGCGGATGTGGATTGAAGAAGGGTTTAGGGACCTGAAGGGGCAGGGG
TTTGGGCTGGACCGCCATCGGCTGCGGACGGGGGCGAGCCTCAGGGGGTGGTTATGGCTTCTGGCCTTGGGGATGGCGCTCTTGATCCTTCTGGGGGCGC
GCTTGCAGGGCAGGGAATGGCTTCCCCGGCTCCTGGCCCATCCCGAGCGGCAGAGCCTCTTCCGTCTGGGCCGGATCGCCCTGGCCCAGGGGCCCCCGCC
TTGGAGGGAAGCGGTGGTGGAGGAGCTGATCAGGTTGCTTCAGGAACTGGGGGGAGGAAAGTGATGAAAGTGATGAGAGCTCAG
CCCTCTGGGTCCATAAGGCCTTCGCCTCCCTCAGGAAAACCATTCGCTCCAACCTCGCCCTCTTCCTCTCCACCCTCCTCACCGCTCCCCTGGACCCCAC
CCTCTCCGACCTCGCCCGCAGAACCCCCCTCCCCACCCTGGCCCAAAGCCGCCTCAATCGCCTCTGGCGCTTCCTCCATCACCCCACCCTGCAAAACCCC
TGGGCCCTCACCGAAGCCCTCCTCCCCCTCCTCGCCCGTCGTTTCCCCAAAGACCGCCCCCTCCCCCTCATCGTGGACTGGACCTTCGCGGAGGACGGCA
GGCACCAAGCCCTGGTGGCCGCCCTTCCCCTCAAGGGAAGGGCCCTGGTGGTGGCCTTCGCTCTTCACCCCCTCTCCCCTTTCCCCAGTCAAAACCGGGT
GGAGGAGGAGTTCCTCCACCGCCTGGGCCGCGCCGTCCAGGACCTGGGATATACCCCCCTCTTCCTCCTGGACCGCGGCTTTGACCGGGTCTCCCTGATG
CGAAAGCTCCAGGGGTGGGGCATGGGCTTCCTCATCCGCCTGCGGCAGAACCGGGAGGTGGAACCCCGAGGGGGGAAGCGCCTTCCCCTGAAGGAGGGCT
ACCGGCGTGTGGTCCACCCCCTGCGGGAGGAGGTCCGCCTTTTCGGACACGGTGGGGAGGAGGTAGAGGTCACCCTCCTGGTGTACCCGGGGGGTCGGGA
GCCCTGGTATCTGGCCTATTCGGGCCCTTTTGGAGGGAAGCCGCCCTACGGGTGGCGGATGTGGATTGAAGAAGGGTTTAGGGACCTGAAGGGGCAGGGG
TTTGGGCTGGACCGCCATCGGCTGCGGACGGGGGCGAGCCTCAGGGGGTGGTTATGGCTTCTGGCCTTGGGGATGGCGCTCTTGATCCTTCTGGGGGCGC
GCTTGCAGGGCAGGGAATGGCTTCCCCGGCTCCTGGCCCATCCCGAGCGGCAGAGCCTCTTCCGTCTGGGCCGGATCGCCCTGGCCCAGGGGCCCCCGCC
TTGGAGGGAAGCGGTGGTGGAGGAGCTGATCAGGTTGCTTCAGGAACTGGGGGGAGGAAAGTGATGAAAGTGATGAGAGCTCAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1095 bp | 365 aa | 67 | 1161 | + | No |
Chemistry : DDE
ORF sequence :
MPPTTPLSQVITLWVHKAFASLRKTIRSNLALFLSTLLTAPLDPTLSDLARRTPLPTLAQSRLNRLWRFLHHPTLQNPWALTEALLPLLARRFPKDRPLP
LIVDWTFAEDGRHQALVAALPLKGRALVVAFALHPLSPFPSQNRVEEEFLHRLGRAVQDLGYTPLFLLDRGFDRVSLMRKLQGWGMGFLIRLRQNREVEP
RGGKRLPLKEGYRRVVHPLREEVRLFGHGGEEVEVTLLVYPGGREPWYLAYSGPFGGKPPYGWRMWIEEGFRDLKGQGFGLDRHRLRTGASLRGWLWLLA
LGMALLILLGARLQGREWLPRLLAHPERQSLFRLGRIALAQGPPPWREAVVEELIRLLQELGGGK
LIVDWTFAEDGRHQALVAALPLKGRALVVAFALHPLSPFPSQNRVEEEFLHRLGRAVQDLGYTPLFLLDRGFDRVSLMRKLQGWGMGFLIRLRQNREVEP
RGGKRLPLKEGYRRVVHPLREEVRLFGHGGEEVEVTLLVYPGGREPWYLAYSGPFGGKPPYGWRMWIEEGFRDLKGQGFGLDRHRLRTGASLRGWLWLLA
LGMALLILLGARLQGREWLPRLLAHPERQSLFRLGRIALAQGPPPWREAVVEELIRLLQELGGGK
Blast result :
Comments
ISTth2 is 41% aa similar to IS10R.
ISTth2 was found by screening completely sequenced genomes for seqences homologous to the ISXaca1 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-64-D(N3)-98-E(C1). The copy number in Thermus thermophilus HB27 is 2 on the chromosome and 1 on plasmid pTT27.
ISTth2 was found by screening completely sequenced genomes for seqences homologous to the ISXaca1 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-64-D(N3)-98-E(C1). The copy number in Thermus thermophilus HB27 is 2 on the chromosome and 1 on plasmid pTT27.
References
1] Henne,A., Bruggemann,H., Raasch,C., Wiezer,A., Hartsch,T., Liesegang,H., Johann,A., Lienard,T., Gohl,O., Martinez-Arias,R., Jacobi,C., Starkuviene,V., Schlenczeck,S., Dencker,S., Huber,R., Klenk,H.P., Kramer,W., Merkl,R., Gottschalk,G. and Fritz,H.J.(2004)Nat. Biotechnol. 22,547-553