ISAzvi5
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001157 | ND | Azotobacter vinelandii | Azotobacter vinelandii AvOP |
DNA section
IS Length : 1328 bp
Ends
IR Length : 18/20
IRL : CCCTGAGTGATCCCCTGAAATTTCCCAGAAAAATCAGTTAGGTAGGGCTT
IRR : CCCTGAGTGATCCCCACAAAATCACCCCAGCTCCAGCGCTTGCCGGTGGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGAGAAGGGT | ACCGA | ACGGAACAGC | 5 |
DNA sequence
CCCTGAGTGATCCCCTGAAATTTCCCAGAAAAATCAGTTAGGTAGGGCTTGAACAGGCCTGCATGGGTGGGCGACGTTGGTTGTCGCCAAACAAAAACAG
CGTCCCCCGCCCCATGCAGACCGTCCAATTCTTGCACGCCGCCTTCGCCGAGGCACTCCCCACGATCCATGCCCGCCGTCTCGAAGCCTTGATGGCGGCG
GTAGCCGCCCTGCTGCAGGGCCGCCGCCTGACCCTGACCGCACTGGGGCGCTCTCTGCCCGGCTCTGCCTGGCCCAGGCAGGCGATCAAGCGGATCGACC
GCCTGCTCGGCAACCGGCAGCTTCAAGCCGAACGTGGCTTGTTCTATTGGGTCATGCTCCGCGCCCTGCTGGGCTCGCTCAGGCATCCGTTGATCCTGGT
GGACTGGTCGCCGATCGATGCCACCGGCAACCTGTTCCTGCTGCGAGCGTCGATGCCGCTGGCCGGGCGCTCGTTGCCGGTCTGCGAAGTCGTCCCCCCG
CGCGAAGGCTGCCCGCGTTGCCAGCGGCGCCTGCTCGAGGCCCTGGCGGCGATGCTGCCGGCCGACTGCACGCCCATCCTGGTCACCGATGCGGGCTTCC
GGCGGCCCTGGTTCCAGGCCGTCGAGGCGCAGGGCTGGCATTACGTCGGCCGCGTGCGCAACCGCGACCTGTGCCGGCTCGGCGAGCAGTCGTGGCAGCC
GGTGAAAAGCCTGTATGCCCTGGCCTCGGCGTCGCCGAAACGGCTCGGTCGTCTCGAGACGACGCGCTCGGCGCCCTGGTCCACCCAGCTCTGCGCGGTG
AAGCATCCATCGCGGGGGCGCAAGCACCGGCGCGTCACCGGACGCGTGGCCCGGGACAAACGCAGCCGGCAGAACGCCCGGCGCGAAAGCGAGCCCTGGC
TGCTGGCCAGCAACCTGCCCGAGGCGCAGTGGAGTGCCGCGCAGATCGTGGCGATCTACAGGCGGCGCAGGCAGGTCGAGGAAGGCTTCCGCGACCTGAA
GAGCCATCGGCTGGGGATCGGTCTGGGGCTGCGTCGCAGCCGCTGCCCGCGGCGCATCGAGATCCTGCTGCTGATCGCCGTGCTGGCCAACTACGCGCTG
TGCCTACTGGGCCTGCAGGCGCGGGAGGCGGGGCACGAACGGCATTTCCAGAGCAACAGCCTCAAGGACCGGCACGTGTTGTCGCTGTGGCGGCTGGGGC
TGGAGTATGCACGCCGTTACGCGGGAGACCTTTCCCGGGAGAAGCTACGGAATCTGGAGCTGGCCCTGCGGCGGGAGGTCCACCGGCAAGCGCTGGAGCT
GGGGTGATTTTGTGGGGATCACTCAGGG
CGTCCCCCGCCCCATGCAGACCGTCCAATTCTTGCACGCCGCCTTCGCCGAGGCACTCCCCACGATCCATGCCCGCCGTCTCGAAGCCTTGATGGCGGCG
GTAGCCGCCCTGCTGCAGGGCCGCCGCCTGACCCTGACCGCACTGGGGCGCTCTCTGCCCGGCTCTGCCTGGCCCAGGCAGGCGATCAAGCGGATCGACC
GCCTGCTCGGCAACCGGCAGCTTCAAGCCGAACGTGGCTTGTTCTATTGGGTCATGCTCCGCGCCCTGCTGGGCTCGCTCAGGCATCCGTTGATCCTGGT
GGACTGGTCGCCGATCGATGCCACCGGCAACCTGTTCCTGCTGCGAGCGTCGATGCCGCTGGCCGGGCGCTCGTTGCCGGTCTGCGAAGTCGTCCCCCCG
CGCGAAGGCTGCCCGCGTTGCCAGCGGCGCCTGCTCGAGGCCCTGGCGGCGATGCTGCCGGCCGACTGCACGCCCATCCTGGTCACCGATGCGGGCTTCC
GGCGGCCCTGGTTCCAGGCCGTCGAGGCGCAGGGCTGGCATTACGTCGGCCGCGTGCGCAACCGCGACCTGTGCCGGCTCGGCGAGCAGTCGTGGCAGCC
GGTGAAAAGCCTGTATGCCCTGGCCTCGGCGTCGCCGAAACGGCTCGGTCGTCTCGAGACGACGCGCTCGGCGCCCTGGTCCACCCAGCTCTGCGCGGTG
AAGCATCCATCGCGGGGGCGCAAGCACCGGCGCGTCACCGGACGCGTGGCCCGGGACAAACGCAGCCGGCAGAACGCCCGGCGCGAAAGCGAGCCCTGGC
TGCTGGCCAGCAACCTGCCCGAGGCGCAGTGGAGTGCCGCGCAGATCGTGGCGATCTACAGGCGGCGCAGGCAGGTCGAGGAAGGCTTCCGCGACCTGAA
GAGCCATCGGCTGGGGATCGGTCTGGGGCTGCGTCGCAGCCGCTGCCCGCGGCGCATCGAGATCCTGCTGCTGATCGCCGTGCTGGCCAACTACGCGCTG
TGCCTACTGGGCCTGCAGGCGCGGGAGGCGGGGCACGAACGGCATTTCCAGAGCAACAGCCTCAAGGACCGGCACGTGTTGTCGCTGTGGCGGCTGGGGC
TGGAGTATGCACGCCGTTACGCGGGAGACCTTTCCCGGGAGAAGCTACGGAATCTGGAGCTGGCCCTGCGGCGGGAGGTCCACCGGCAAGCGCTGGAGCT
GGGGTGATTTTGTGGGGATCACTCAGGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1242 bp | 414 aa | 63 | 1304 | + | No |
Chemistry : DDE
ORF sequence :
MGGRRWLSPNKNSVPRPMQTVQFLHAAFAEALPTIHARRLEALMAAVAALLQGRRLTLTALGRSLPGSAWPRQAIKRIDRLLGNRQLQAERGLFYWVMLR
ALLGSLRHPLILVDWSPIDATGNLFLLRASMPLAGRSLPVCEVVPPREGCPRCQRRLLEALAAMLPADCTPILVTDAGFRRPWFQAVEAQGWHYVGRVRN
RDLCRLGEQSWQPVKSLYALASASPKRLGRLETTRSAPWSTQLCAVKHPSRGRKHRRVTGRVARDKRSRQNARRESEPWLLASNLPEAQWSAAQIVAIYR
RRRQVEEGFRDLKSHRLGIGLGLRRSRCPRRIEILLLIAVLANYALCLLGLQAREAGHERHFQSNSLKDRHVLSLWRLGLEYARRYAGDLSREKLRNLEL
ALRREVHRQALELG
ALLGSLRHPLILVDWSPIDATGNLFLLRASMPLAGRSLPVCEVVPPREGCPRCQRRLLEALAAMLPADCTPILVTDAGFRRPWFQAVEAQGWHYVGRVRN
RDLCRLGEQSWQPVKSLYALASASPKRLGRLETTRSAPWSTQLCAVKHPSRGRKHRRVTGRVARDKRSRQNARRESEPWLLASNLPEAQWSAAQIVAIYR
RRRQVEEGFRDLKSHRLGIGLGLRRSRCPRRIEILLLIAVLANYALCLLGLQAREAGHERHFQSNSLKDRHVLSLWRLGLEYARRYAGDLSREKLRNLEL
ALRREVHRQALELG
Blast result :
Comments
ISAzvi5 is 57% aa similar to IS10R.
ISAzvi5 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-61-D(N3)-129-E(C1).
Genome in progress : Accession number : NZ_AAAU03000027.1
ISAzvi5 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-61-D(N3)-129-E(C1).
Genome in progress : Accession number : NZ_AAAU03000027.1
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2005)Unpublished
2] De Palmenaer,D. et al.(2006)Unpublished
2] De Palmenaer,D. et al.(2006)Unpublished