ISfinder Mirror Site

ISDpr2

Family IS4
Group ISPepr1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	delta proteobacterium	delta proteobacterium MLMS-1

DNA section

IS Length : 1549 bp

Ends

IR Length : 17

IRL : CTCAACTTTCGGAGTTGCCGACCAGTAGCCCCATTTTTTATGGTAAAATG
IRR : CTCAACTTTCGGAGTTGAGGTAAATGGTTGATTTTACGAAGCTGGTGCCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATCATAGTT		CTATCAGGTT	0

DNA sequence

CTCAACTTTCGGAGTTGCCGACCAGTAGCCCCATTTTTTATGGTAAAATGCTGAAAACACATAATATTTAACGTGAAATGCTCTTCGCTTTTTGATATAT
TGGCTTTTGCGAAAAAATCAATAAAATCAACAAGGAAGAGCACAAATGGATACTACCAGCCAGTTCGAAATAGAGCAAGAAGCAAAACGCCTGCAAAGCG
CAATCAAATCGTTCTTTGACAATTTTTCGATAGGCACCCTGCTGAATCGATCCGGCATCAAGAAAGTCAGGGGTGCCACCCCACTGGCCGTATTCGAGGC
CATCTTCACGCTGGCTTTCCGTGGACAGAATTTCTATCGGGGCATTGTTCTGAATGGCGAGCTGGGGTTCCAAAAAGATGCGGCCTACGAGCTGTTGGAA
AATCCCCGCTACAATTGGCGGCAGTTTCTGCTCAAGCTGGCCATGAAAATAATCGGAGCTATTGATTTGTTGACCAGCGCAGAGCGAGAGAGGGTGCTGG
TAGTTGACGATAGCGATTATGATCGTGCCCGCTCCAAGAAGGTAGAGTTGCTGGCCAAGATATTCGACCACAACAGCGGGAAGTATCTGAAGGGGTTCAA
GCTGCTTACCCTCGGCTGGGGTGATGGCGTAACCTTTTTGCCTTTGGACTTTGTGCTGAGGTCGTCGGCCAACGCGGTCAACAGAATCCAGGGTATCACC
AAGGATTTGGACAAGCGGACTTGCGGGTATAAACGTCGTATTGAGGCCATGACCAAATCCACCGACGCGCTTGAGGTGATGGTGAAAAGGGTCATGGCAT
TGGGGGTCAGGGCCGACTATCTCCTGATGGATAGCTGGTTCTGCTTTGCCAGCCTGATCAGAAAGCTCTCGACTCATTTGCCGGTCATCTGCATGGCCAA
AGACCTCAAGAGCAACTTCTACCAATACCGGGGTGAATCTCTCCGACTTGGCAGACTGTATCAGCAGCTGAAAAAACGACCGGGCAGAGCTCAAATTCTT
GCCAGTATTGTCTTGTCCATGGTGAATGGCCCTACGGTCAAGCTTGTCTTCGTCAGAAATCGCAACGGCAGGGGTTGGCTGGCGCTTCTGTCCACCGACA
CGACTTTGCCCGACGAGGAGATCATCAGAATTTACGGCAAGCGTTGGGATATCGAGGTGTTTTTTAAGATGGCCAAGCACCACTTGAATTTGGAGCGGGA
GGTTCAGTTGCGGGACTACGACGGACTCGTGGCCCACACCTCTCTGGTGTTCACCCGCTACCTGTTTTTGGCGTTGCAGCAAAGGTTCCATGATGATCCA
CGATCCATTGGTAGCCTGTTCTACGCCTGCTGCGATGAAATGCAGGACCTGACGCTGTTTGAAGCACTCCACCGGCTGCTGACCCTGACCCTGGAAAAGG
TCAGGGCCTCAAAGGAGTTCGCAGAAAGCGCAATAAACGCAATGATTGATGCAATCATGGGCACTGCAATTGATATGATTAAATCAGCCCAAAAGATGGC
GGCACCAGCTTCGTAAAATCAACCATTTACCTCAACTCCGAAAGTTGAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1368 bp	456 aa	146	1513	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDTTSQFEIEQEAKRLQSAIKSFFDNFSIGTLLNRSGIKKVRGATPLAVFEAIFTLAFRGQNFYRGIVLNGELGFQKDAAYELLENPRYNWRQFLLKLAM
KIIGAIDLLTSAERERVLVVDDSDYDRARSKKVELLAKIFDHNSGKYLKGFKLLTLGWGDGVTFLPLDFVLRSSANAVNRIQGITKDLDKRTCGYKRRIE
AMTKSTDALEVMVKRVMALGVRADYLLMDSWFCFASLIRKLSTHLPVICMAKDLKSNFYQYRGESLRLGRLYQQLKKRPGRAQILASIVLSMVNGPTVKL
VFVRNRNGRGWLALLSTDTTLPDEEIIRIYGKRWDIEVFFKMAKHHLNLEREVQLRDYDGLVAHTSLVFTRYLFLALQQRFHDDPRSIGSLFYACCDEMQ
DLTLFEALHRLLTLTLEKVRASKEFAESAINAMIDAIMGTAIDMIKSAQKMAAPAS

Blast result :

Comments

ISDpr2 is 65% aa similar to ISDha5. ISDpr2 was found by screening completely sequenced genomes for sequences homologous to the ISGur1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-106-D(N3)-107-E(C1). The sequencing of the Delta proteobacterium MLMS-1 chromosome is currently in progress. Accession: AAQF00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P.(2006)Unpublished