ISBf8
- Family IS4
- Group ISPepr1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U75371 | ND | Bacteroides fragilis | Bacteroides fragilis |
DNA section
IS Length : 1663 bp
Ends
IR Length : 19/23
IRL : CTAAAGTTTCCCACCCAAATAAATAGATAGAAAAATAACAGTTTGTCGAA
IRR : CTAAAGTTTCCCACCCCTTAAAACACTAACTTTTCTCATTTTCCTTTGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGACAAAGG | TACATAA | CTTTACGGAA | 7 |
DNA sequence
CTAAAGTTTCCCACCCAAATAAATAGATAGAAAAATAACAGTTTGTCGAATTTTCTTTGTAAATTAGTAATCGCTAAAGAACTGATTTTAAAGACAAGAC
AACAAAACTGTTATGGAAGCAAAAATAGAGAAAATAAGTGAGTTATCCAAACTTTTGAGTGTTAAAACCCGAATGAGTGATGATTTATTTCATCTTTTTG
GCAAGTTTGGCATCGGTCACCTATTATCTCGCCTTTCATTGGAGAAACAGGACGGAGTTTCGGCTTCGGAGTTGCTCCTCTCTCTTTGCCTCTTCCGCAT
TGTGGGCGAGAGCATCCATAGTATATGCAAACATAAGATATATGAGCTTTCAAATCATGGTAAGAACTGTTTCTATCGCATGATGATTCGCCCACAGATG
GATTGGAGACGATTGATGAACCACTTTGCACTGCGTTATATGTGCCTATTGCGTAAGTATGGCGAAGTTCCTCAATCAGATACCACTACATGTTTCATTA
TAGATGACACCGTGCTTGAGAAGAGTGGTGTGAGGATGGAGGGTATCAGTCGTGTTTTCGACCATATGAAAGGCAGATGCGTATTGGGCTACAAGCTGTT
ACTTTGTGCCTTCTTTGACGGCAAGACAACTATACCCTTTGATTTTTCACTACATCAAGAAAAGGGGAAGCAAGGCAACTGCGGGCTGACAAGACAGCAA
CTCAAAAAGGCATATCACACCAAGAGGAACACCGGCAATCCTGATTATAAGCGCTTTCAAGAGTGTAAGATGTCTAAGATGGAAGTTGCCATGGATATGC
TTCGCCGTGGATGGAAGATGGGGTTACATGCGAAGTATGTGATTACCGATAGTTGGTTCACCTGCGAGCAACTTATGACATGTGTTAGAAGCATAGGTAA
AGGGGCAATGCACTTTGTTGGACTTGCAAAAATGGGAAAGACAAAATACACTATATCGGGCAAAAAGAAAAATGCTGCAGAACTCATTGCCACCTATGAA
CGTGAACGAGGAAAGAACTGTCGTAAATACAAATGTCGATATATTCAGCTCAACGGCAACTTAGGAGATATACCTATCAGAATCTTCCTCATCAAGTATG
GTAGAAACTCCGCATGGAACGTTCTGCTCACCACGGATACAACGATGTATTTCGTAAAAGCCTTTGAAGTGTATCAGATTAGATGGAACATAGAGGTGAT
GAACAAGGAGACTAAGCAATATCTCGGATTAGGAGGTTATCAAGGTTGTGACTTTAATGGTCAGATAGCCGACGCAACGCTGTGTTACCTTACATATACC
GTCATGGCTTTGGAAAAGAGATTCACAGAATATCAAACCATGGGCGAACTCTTTTCGGATATGGAGGGTGATCTCATGGCACTCACGTTATGGAAGCGAG
TTCTTACCTGCATCGAACGCATTCTTCGCATTTTAGGAGAAATACTTGGAATGACGCCCCAATACCTTATGGCTACAATCAGCGGAAACGACAAAGAGAT
GAGCAAAATCCTTGTAATGGCTGAAGCGTTGGAAAAATGGGACGAAGTATGTGGATAGTCTGCATAACTTCTGTTAGGCATGTGTTAACAGTCAATGGAT
ATGGGGGAAGACAGACAAAGGAAAATGAGAAAAGTTAGTGTTTTAAGGGGTGGGAAACTTTAG
AACAAAACTGTTATGGAAGCAAAAATAGAGAAAATAAGTGAGTTATCCAAACTTTTGAGTGTTAAAACCCGAATGAGTGATGATTTATTTCATCTTTTTG
GCAAGTTTGGCATCGGTCACCTATTATCTCGCCTTTCATTGGAGAAACAGGACGGAGTTTCGGCTTCGGAGTTGCTCCTCTCTCTTTGCCTCTTCCGCAT
TGTGGGCGAGAGCATCCATAGTATATGCAAACATAAGATATATGAGCTTTCAAATCATGGTAAGAACTGTTTCTATCGCATGATGATTCGCCCACAGATG
GATTGGAGACGATTGATGAACCACTTTGCACTGCGTTATATGTGCCTATTGCGTAAGTATGGCGAAGTTCCTCAATCAGATACCACTACATGTTTCATTA
TAGATGACACCGTGCTTGAGAAGAGTGGTGTGAGGATGGAGGGTATCAGTCGTGTTTTCGACCATATGAAAGGCAGATGCGTATTGGGCTACAAGCTGTT
ACTTTGTGCCTTCTTTGACGGCAAGACAACTATACCCTTTGATTTTTCACTACATCAAGAAAAGGGGAAGCAAGGCAACTGCGGGCTGACAAGACAGCAA
CTCAAAAAGGCATATCACACCAAGAGGAACACCGGCAATCCTGATTATAAGCGCTTTCAAGAGTGTAAGATGTCTAAGATGGAAGTTGCCATGGATATGC
TTCGCCGTGGATGGAAGATGGGGTTACATGCGAAGTATGTGATTACCGATAGTTGGTTCACCTGCGAGCAACTTATGACATGTGTTAGAAGCATAGGTAA
AGGGGCAATGCACTTTGTTGGACTTGCAAAAATGGGAAAGACAAAATACACTATATCGGGCAAAAAGAAAAATGCTGCAGAACTCATTGCCACCTATGAA
CGTGAACGAGGAAAGAACTGTCGTAAATACAAATGTCGATATATTCAGCTCAACGGCAACTTAGGAGATATACCTATCAGAATCTTCCTCATCAAGTATG
GTAGAAACTCCGCATGGAACGTTCTGCTCACCACGGATACAACGATGTATTTCGTAAAAGCCTTTGAAGTGTATCAGATTAGATGGAACATAGAGGTGAT
GAACAAGGAGACTAAGCAATATCTCGGATTAGGAGGTTATCAAGGTTGTGACTTTAATGGTCAGATAGCCGACGCAACGCTGTGTTACCTTACATATACC
GTCATGGCTTTGGAAAAGAGATTCACAGAATATCAAACCATGGGCGAACTCTTTTCGGATATGGAGGGTGATCTCATGGCACTCACGTTATGGAAGCGAG
TTCTTACCTGCATCGAACGCATTCTTCGCATTTTAGGAGAAATACTTGGAATGACGCCCCAATACCTTATGGCTACAATCAGCGGAAACGACAAAGAGAT
GAGCAAAATCCTTGTAATGGCTGAAGCGTTGGAAAAATGGGACGAAGTATGTGGATAGTCTGCATAACTTCTGTTAGGCATGTGTTAACAGTCAATGGAT
ATGGGGGAAGACAGACAAAGGAAAATGAGAAAAGTTAGTGTTTTAAGGGGTGGGAAACTTTAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1443 bp | 481 aa | 113 | 1555 | + | No |
Chemistry : DDE
ORF sequence :
MEAKIEKISELSKLLSVKTRMSDDLFHLFGKFGIGHLLSRLSLEKQDGVSASELLLSLCLFRIVGESIHSICKHKIYELSNHGKNCFYRMMIRPQMDWRR
LMNHFALRYMCLLRKYGEVPQSDTTTCFIIDDTVLEKSGVRMEGISRVFDHMKGRCVLGYKLLLCAFFDGKTTIPFDFSLHQEKGKQGNCGLTRQQLKKA
YHTKRNTGNPDYKRFQECKMSKMEVAMDMLRRGWKMGLHAKYVITDSWFTCEQLMTCVRSIGKGAMHFVGLAKMGKTKYTISGKKKNAAELIATYERERG
KNCRKYKCRYIQLNGNLGDIPIRIFLIKYGRNSAWNVLLTTDTTMYFVKAFEVYQIRWNIEVMNKETKQYLGLGGYQGCDFNGQIADATLCYLTYTVMAL
EKRFTEYQTMGELFSDMEGDLMALTLWKRVLTCIERILRILGEILGMTPQYLMATISGNDKEMSKILVMAEALEKWDEVCG
LMNHFALRYMCLLRKYGEVPQSDTTTCFIIDDTVLEKSGVRMEGISRVFDHMKGRCVLGYKLLLCAFFDGKTTIPFDFSLHQEKGKQGNCGLTRQQLKKA
YHTKRNTGNPDYKRFQECKMSKMEVAMDMLRRGWKMGLHAKYVITDSWFTCEQLMTCVRSIGKGAMHFVGLAKMGKTKYTISGKKKNAAELIATYERERG
KNCRKYKCRYIQLNGNLGDIPIRIFLIKYGRNSAWNVLLTTDTTMYFVKAFEVYQIRWNIEVMNKETKQYLGLGGYQGCDFNGQIADATLCYLTYTVMAL
EKRFTEYQTMGELFSDMEGDLMALTLWKRVLTCIERILRILGEILGMTPQYLMATISGNDKEMSKILVMAEALEKWDEVCG
Blast result :
Comments
ISBf8 is 56% aa similar to ISUnCu6. ISBf8 is inserted in the Tn4555 transposon from Bacteroides fragilis. It was found by screening for sequences homologous to the ISGur1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-113-D(N3)-114-E(C1).
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Tribble,G.D., Parker,A.C. and Smith,C.J.(1997)J. Bacteriol. 179 (8), 2731-2739
3] Soki J, Fodor E, Hecht DW, Edwards R, Rotimi VO, Kerekes I, Urban E, Nagy E (2004)Journal of Medical Microbiology 53: 413-419.
2] Tribble,G.D., Parker,A.C. and Smith,C.J.(1997)J. Bacteriol. 179 (8), 2731-2739
3] Soki J, Fodor E, Hecht DW, Edwards R, Rotimi VO, Kerekes I, Urban E, Nagy E (2004)Journal of Medical Microbiology 53: 413-419.