ISVvu2
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004459 | ND | Vibrio vulnificus | Vibrio vulnificus CMCP6 |
DNA section
IS Length : 1430 bp
Ends
IR Length : 16/18
IRL : TAATGCCGATCAGTTAAGAGATAAATGATCGGTTGAACGATCCTGCAGAT
IRR : TAATGCCAGTCAGTTAAGCTGACTGGCTTTTTTTATTGGGGTATTTCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAAAACGA | ACCCAGCGGT | TCGTTTTTTA | 10 |
| GAAAAATGGG | AGCCTTGGCT | CCCATTTTTG | 10 |
| ACAAAAAAGC | GCCACAATGGC | GCTTTTTTAT | 11 |
| GATAATGAGA | GGCATTGAGGT | CATTTAATTG | 11 |
| TAAAAATACC | GGCACAGAGGC | CGGTATTTTT | 11 |
DNA sequence
TAATGCCGATCAGTTAAGAGATAAATGATCGGTTGAACGATCCTGCAGATGGCTTATAAAGGCTCTAAGTTTCTAAAACTTTGAGCCTTTTATTTTGCAA
TTAACTACAGCCCTAACACTCGCCAATCGTTATGCCCCCAATACTGAACAACTTGGAAAACTCAGTGACATTCTTTGCCCTGATTTCATCAACCAATGCT
TAGAAGCTTCCGGTGTGGCCACTATTCGTAAACGTCGTATCCCTCTCGACATGGCCGTTTGGGCGGTCGTTGCTATGTCGCTTTACCGACAAGAGCCTTT
GTGGTCAATTGTCTCCAAAGCGCAATTAATGTTGCCCGGGAAGCGAAGTCTGGTTGCCCCTAGTGCTATCGTTCAAGCGAGACAACGGCTCGGGGCTGAT
GCAATGAAAGAAGTATTTCATCAAAGCCAGAGCTTATGGAATGAAACAGCTGACCATCCGACTTGGTGTGGATTAAAACTCCTCGCTGTCGATGGCGTGG
TATGGCGAACGCCAGACACTAAGGAAAACCGTGATGCGTTTCAATCTGCGTCAAATCAGAATGGTGAAGGTAGCTTTCCTCAGGTGCGTATGGTTTGCCA
GATGGAGTTGACCAGTCACATGCTGGTCGCCAGTGCTTTCGCGAGTTACAAAACCAATGAGATGATATTGGCAGAACAGCTGATTGAAACAACACCAGAC
TACAGCCTCACAATGTTCGACAGAGGTTTTTACTCTCTTAGCCTTCTTCATCGTTGGGCAAATACAGGTAATGAAAGGCATTGGCTAATGCCAATGCGCA
AGAATACCCAATTTACTGAAGTTAGAAAGCTGGGTCGCAATGATCGGATTGTCGAACTCAAGACAACGCCTCAAGCGAGAAAGAAGTCCCTGTCACTACC
TGAAACCATCGAAGTTCGCCTGATAAAGAAAACTATCAAAGGGAAAGAAGTGAGTATCTTGACCTCGATGACTGATCATCGTCGTTATCCGCCAGCGGAA
ATAGCAGAGCTTTACAGTCATAGATGGGAAATCGAAGTGGGATATCGTGAAATGAAGTCTTCACTTCTGAACAACGAGTTCACACTGAGAAGTAAGAAGC
CAGAGATGGTAAAACAAGAACTATGGGGCTTATTGCTTAGCTATAACATTATTAGATATCAGATGGTTAACATGGCTAAAGCTGTGCCTGGCATCTACCC
CAACCAACTGAGCTTCACAACGTGTGCTCACTCTATCATTCACGTAATTTTGGGGTTCTGGTTGGAGTCAGCGGGAACAATCCCTAAGCGCATTACTCAC
TTGCAAGAGGAGGCGATACACCATGTCTTGTCCATTAAACGAGAAGAGCGGATATATCCGAGAGCAATAAAACCAAAGGCAAAGAAATACCCCAATAAAA
AAAGCCAGTCAGCTTAACTGACTGGCATTA
TTAACTACAGCCCTAACACTCGCCAATCGTTATGCCCCCAATACTGAACAACTTGGAAAACTCAGTGACATTCTTTGCCCTGATTTCATCAACCAATGCT
TAGAAGCTTCCGGTGTGGCCACTATTCGTAAACGTCGTATCCCTCTCGACATGGCCGTTTGGGCGGTCGTTGCTATGTCGCTTTACCGACAAGAGCCTTT
GTGGTCAATTGTCTCCAAAGCGCAATTAATGTTGCCCGGGAAGCGAAGTCTGGTTGCCCCTAGTGCTATCGTTCAAGCGAGACAACGGCTCGGGGCTGAT
GCAATGAAAGAAGTATTTCATCAAAGCCAGAGCTTATGGAATGAAACAGCTGACCATCCGACTTGGTGTGGATTAAAACTCCTCGCTGTCGATGGCGTGG
TATGGCGAACGCCAGACACTAAGGAAAACCGTGATGCGTTTCAATCTGCGTCAAATCAGAATGGTGAAGGTAGCTTTCCTCAGGTGCGTATGGTTTGCCA
GATGGAGTTGACCAGTCACATGCTGGTCGCCAGTGCTTTCGCGAGTTACAAAACCAATGAGATGATATTGGCAGAACAGCTGATTGAAACAACACCAGAC
TACAGCCTCACAATGTTCGACAGAGGTTTTTACTCTCTTAGCCTTCTTCATCGTTGGGCAAATACAGGTAATGAAAGGCATTGGCTAATGCCAATGCGCA
AGAATACCCAATTTACTGAAGTTAGAAAGCTGGGTCGCAATGATCGGATTGTCGAACTCAAGACAACGCCTCAAGCGAGAAAGAAGTCCCTGTCACTACC
TGAAACCATCGAAGTTCGCCTGATAAAGAAAACTATCAAAGGGAAAGAAGTGAGTATCTTGACCTCGATGACTGATCATCGTCGTTATCCGCCAGCGGAA
ATAGCAGAGCTTTACAGTCATAGATGGGAAATCGAAGTGGGATATCGTGAAATGAAGTCTTCACTTCTGAACAACGAGTTCACACTGAGAAGTAAGAAGC
CAGAGATGGTAAAACAAGAACTATGGGGCTTATTGCTTAGCTATAACATTATTAGATATCAGATGGTTAACATGGCTAAAGCTGTGCCTGGCATCTACCC
CAACCAACTGAGCTTCACAACGTGTGCTCACTCTATCATTCACGTAATTTTGGGGTTCTGGTTGGAGTCAGCGGGAACAATCCCTAAGCGCATTACTCAC
TTGCAAGAGGAGGCGATACACCATGTCTTGTCCATTAAACGAGAAGAGCGGATATATCCGAGAGCAATAAAACCAAAGGCAAAGAAATACCCCAATAAAA
AAAGCCAGTCAGCTTAACTGACTGGCATTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1320 bp | 440 aa | 95 | 1414 | + | No |
Chemistry : DDE
ORF sequence :
LQLTTALTLANRYAPNTEQLGKLSDILCPDFINQCLEASGVATIRKRRIPLDMAVWAVVAMSLYRQEPLWSIVSKAQLMLPGKRSLVAPSAIVQARQRLG
ADAMKEVFHQSQSLWNETADHPTWCGLKLLAVDGVVWRTPDTKENRDAFQSASNQNGEGSFPQVRMVCQMELTSHMLVASAFASYKTNEMILAEQLIETT
PDYSLTMFDRGFYSLSLLHRWANTGNERHWLMPMRKNTQFTEVRKLGRNDRIVELKTTPQARKKSLSLPETIEVRLIKKTIKGKEVSILTSMTDHRRYPP
AEIAELYSHRWEIEVGYREMKSSLLNNEFTLRSKKPEMVKQELWGLLLSYNIIRYQMVNMAKAVPGIYPNQLSFTTCAHSIIHVILGFWLESAGTIPKRI
THLQEEAIHHVLSIKREERIYPRAIKPKAKKYPNKKSQSA
ADAMKEVFHQSQSLWNETADHPTWCGLKLLAVDGVVWRTPDTKENRDAFQSASNQNGEGSFPQVRMVCQMELTSHMLVASAFASYKTNEMILAEQLIETT
PDYSLTMFDRGFYSLSLLHRWANTGNERHWLMPMRKNTQFTEVRKLGRNDRIVELKTTPQARKKSLSLPETIEVRLIKKTIKGKEVSILTSMTDHRRYPP
AEIAELYSHRWEIEVGYREMKSSLLNNEFTLRSKKPEMVKQELWGLLLSYNIIRYQMVNMAKAVPGIYPNQLSFTTCAHSIIHVILGFWLESAGTIPKRI
THLQEEAIHHVLSIKREERIYPRAIKPKAKKYPNKKSQSA
Blast result :
Comments
ISVvu2 is 68% aa similar to IS4.
ISVvu2 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-75-D(N3)-104-E(C1).The copy number of ISVvu2 in Vibrio vulnificus CMCP6 is 2 on chromosome I and 3 on chromosome II.
ISVvu2 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-75-D(N3)-104-E(C1).The copy number of ISVvu2 in Vibrio vulnificus CMCP6 is 2 on chromosome I and 3 on chromosome II.
References
1] Kim,Y.R., Lee,S.E., Kim,C.M., Kim,S.Y., Shin,E.K., Shin,D.H., Chung,S.S., Choy,H.E., Progulske-Fox,A., Hillman,J.D., Handfield,M. and Rhee,J.H. (2003) Infect. Immun. 71,5461-5471