ISMfl1

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Mycobacterium flavescens
Mycobacterium flavescens PYR-GCK
DNA section
IS Length : 1457 bp

Ends


IR Length : 16

IRL : CAATACCGTTCAGTTAAGACTACATGGGTGTAGTTTGTTGGTATGCCTCG
IRR : CAATACCGTTCAGTTAGTGGCTGATGACGGTGACGCTGGGTGGGTGTTCG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGACCTGCAGGCCTAACGCACCAGAGCCA10

DNA sequence

CAATACCGTTCAGTTAAGACTACATGGGTGTAGTTTGTTGGTATGCCTCGCGCGGGTTGGCGGAAGCCTGAATCGGATCGTCGGTTGTCGGATCTGGTGT
CTGTGGGTGTCCTGACGAGGGTGTTCCCGCCGGCGATGGTTGATGAGGTGATCGAGGCGACGGGGCGTACTCAGGTGCGTCATCGTGCACTGCCGGCACG
GGTGATGGCGTATTTCGCCATCGGCATGGGCCTGTACTCCGATGGCTCCTACGAGGATGTGTTATCCCAGCTCACGGATGGTTTAGCGTGGGCTTCCGGG
TGGCGTGAGCAGTATCAGTTGCCGGGGAAGTCGGCCATTTTCCAGGCGCGGGAGCGATTGGGGTCCCAGCCGTTGGCTGCGTTGTTCGCGAGGGTCGCTC
GTCCGTTGGGCGCGGCGGATACGCCGGGGACGTGGGTGGCCGGACGCAGGGTGGTCGCGATCGACGGGACGTGCCTGGATGTGGCGGATAGCCCGGTCAA
TGAAGAGTTCTTCGGGCGTCCGGGGGTGAACAAGGGTGAGAAGTCGGCGTTTCCGCAGGCGCGGTTGTTGGCGGTCGCCGAGTGCGGCACTCATGCGATC
TTCGCCGCCACGATCGGCGCCTACCGCGACGCGGAGTCCACGATGGTCGAGCACGTGCTCGATGCACTGACACCGGAAATGCTCGTGCTGGCTGATCGTG
GATTCTTCTCATACGCGTTGTGGCGTAACGCTTCCGATACCGGAGCCGATCTGCTGTGGCGCGTGAGCACCGGACGCAACGGCCCGACCCCCACTCACGT
CGAGGATCTCGCCGACGGTTCGTGGCTGGCCCACCTGCGCGCGGCCAAGGACCGACACGGCGAGCCAATGCTGGCTCGCGTCATCGACTACACCGTCGAC
GACGGCCGCGACAACCCCGTGGCCTACCGGTTGCTCACCACGCTGACCGACCCGGACACCGCACCCGCGGTCGAGTTGGCGGCCGCGTACGCCCAGCGCT
GGGAGATCGAGAGCGTCTTCGACGAACTCAAGACCCACCAGCGTGGGTCGAAGGTGGTGCTGCGGTCGAAGTCGCCCGACCTCGTCCTGCAGGAGATCTG
GGGATACCTGTGCTGCCACTACGCGATTCGCTCGTTGATGAGCCAAGCCGCACACCACAGCGGACACGACCCGGACCGACTCAGCTTCACCGCCGCGTTG
CGCGTCGTGCGCCAATCCGTCGCCCATCAGGGCGCTTTTTCCCCCTAACCGGCGCCGATCCGCCGACCCTCACTGGCAGGCGTTCCTCCGTCGCCTCCTC
GACCGCATCAACCCGGCACGCCGCACACGGTCAGCTCCCCGCGTGATCAAACGCAAAATGCCCAAGTGGCATGTCAAAAGATCAGCGCACCAACACTGGC
CACAACCCGAACACCCACCCAGCGTCACCGTCATCAGCCACTAACTGAACGGTATTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1203 bp401 aa431245+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRAGWRKPESDRRLSDLVSVGVLTRVFPPAMVDEVIEATGRTQVRHRALPARVMAYFAIGMGLYSDGSYEDVLSQLTDGLAWASGWREQYQLPGKSAIF
QARERLGSQPLAALFARVARPLGAADTPGTWVAGRRVVAIDGTCLDVADSPVNEEFFGRPGVNKGEKSAFPQARLLAVAECGTHAIFAATIGAYRDAEST
MVEHVLDALTPEMLVLADRGFFSYALWRNASDTGADLLWRVSTGRNGPTPTHVEDLADGSWLAHLRAAKDRHGEPMLARVIDYTVDDGRDNPVAYRLLTT
LTDPDTAPAVELAAAYAQRWEIESVFDELKTHQRGSKVVLRSKSPDLVLQEIWGYLCCHYAIRSLMSQAAHHSGHDPDRLSFTAALRVVRQSVAHQGAFS
P

 

Blast result :
Comments
ISMfl1 is 69% aa similar to ISCef4. ISMfl1 was found by screening completely sequenced genomes for sequences homologous to the ISRso13 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-104-E(C1). The sequencing of the Mycobacterium flavescens PYR-GCK chromosome is currently in progress. Accession: NZ_AAPA00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Unpublished