ISCef4
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004369 | ND | Corynebacterium efficiens | Corynebacterium efficiens YS-314 |
DNA section
IS Length : 1451 bp
Ends
IR Length : 19/20
IRL : CAATGCCGTTAAGTTAGTGGGTGTAGTGTTGTGCTATGCCTCGACATGGA
IRR : CAATACCGTTAAGTTAGTGGTGGGATGACGGTGACCACTGGGCCGAGTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATTCGGTG | GGGAAAAGCC | TCCTGCAGTG | 10 |
| GGTGGATCCA | GACTTAAGCG | CTTCCTCTTG | 10 |
| CCTTGCGGGT | GGCGTAGACC | CAGTTCTTCA | 10 |
| TGCGTGACAC | GATCTAATCC | CACCTACTCC | 10 |
| GTGGTGTCAT | CGGTTAAACC | TGAGATCACT | 10 |
| GAAACCACTG | TGTTTAACCC | TGAGATCACT | 10 |
| CAGCCTGCAC | GGGTTAAGCN | CGGTCGCACG | 10 |
DNA sequence
CAATGCCGTTAAGTTAGTGGGTGTAGTGTTGTGCTATGCCTCGACATGGACGGACACAACCTGATACTGATCGTCGGTTATCGGATTTAATCTCGGTGGG
CCTGCTCACCCGGGTGTTTCCGGCCGATGTCGTGGACGAGGTCGTCGCGCAGGCCGGTCGTACCCAGCAACGATCCCGCATCCTGCCCTCCCGGGTAATG
GTGTATTACACCATCACAATGGCTTTGAACTCCGATGGATCCTATGAGGATATCTACGCCGATCTCACCGATGGACTGTCCTGGGCCTGCAGGTGGGAGG
AACAGGCAGTACCTGCTCCGTCACGCTCCGCGATTTTCCAGGCCAGGGTCAGGCTGGGACATGAACCGATGGCAGCGTTGTTTGCCCGTGTTGCCCAACC
ATTGGCCCGGCCGGGGTGGGAGGGGGCCTGGCTGGCGGGCAAACGCCTGTTGGCTGTTGATGGCACCACCTTGGATGTCCCGGATACGCCAGCCAATGTC
GAGCATTTCGGGCGTCCCGGCACCGGACGTGGTCAGGCCGGGTACCCGCAGGCCCGTGTCGTGGCGATCGCGGAATGTGGATCACATGCGATCCTGGACG
CGGTCATCGGCGGGCTGGGCACCGGGGAAACGACCCTGGCTGATGAACTGATCGACCGGCTGGATCCAGGCTCAGTACTGGTCGCTGATCGGGGGTTTTA
TAGTTTCCACCGGTGGGCTACCGCCCAGGACCGTGGTGCGGATCTGGTGTGGCGGATCCGGAACAATGTGAAACCACACCATGTGCGAACCCTGGAGGAT
GGAACATGGTTGGCCACGATTACCCCGAGCAGTGGGCCGGGGTGGCGGCAGGTTGCCCCAAGGATGGTGCGGGTGGTGGACTACCACATCGATACCGGTG
ACGATGAGGCTGGCATTGTCGACCAGCAGCATCGGTTGTTGACCACCTTGTTGGATCCTGATGAGGTGTCGGTGGAGGATCTGATCGCGGCGTATCACGC
CCGGTGGGAGATCGAGAACACTTTCGATGAGTTGAAAACCCATCAGCGTGGACGAGAAAGGGTGTTGCGGTCAAAGTCCCCGGAGTTGGTGCGTCAGGAG
ATCTGGGGGCATCTGTGCTGCCACTACGCGATCAGGACACTGATGGCTGATGCGGCCACTGCCAGTGAGCACCCTCCGGTGCGGGTGTCATTTGTTAAGG
CCTTGAAGCTTACCCGTCGTTCGGTGATCCAGGGCTCTTTTCCCCCCTGACGACCCGCACACCGCCCGAAGTTTATGGGATCGGGCAGTGTCTTGGCTGG
CCAGGTACCTCAACCCACCGCGACGAAGGCGGAAGAATCCGCGGGTGATTAAACGAAAGATGTCGAAATGGCCGGTGAAACGATCCCATCATTGTGGTCG
GCCACTCGGCCCAGTGGTCACCGTCATCCCACCACTAACTTAACGGTATTG
CCTGCTCACCCGGGTGTTTCCGGCCGATGTCGTGGACGAGGTCGTCGCGCAGGCCGGTCGTACCCAGCAACGATCCCGCATCCTGCCCTCCCGGGTAATG
GTGTATTACACCATCACAATGGCTTTGAACTCCGATGGATCCTATGAGGATATCTACGCCGATCTCACCGATGGACTGTCCTGGGCCTGCAGGTGGGAGG
AACAGGCAGTACCTGCTCCGTCACGCTCCGCGATTTTCCAGGCCAGGGTCAGGCTGGGACATGAACCGATGGCAGCGTTGTTTGCCCGTGTTGCCCAACC
ATTGGCCCGGCCGGGGTGGGAGGGGGCCTGGCTGGCGGGCAAACGCCTGTTGGCTGTTGATGGCACCACCTTGGATGTCCCGGATACGCCAGCCAATGTC
GAGCATTTCGGGCGTCCCGGCACCGGACGTGGTCAGGCCGGGTACCCGCAGGCCCGTGTCGTGGCGATCGCGGAATGTGGATCACATGCGATCCTGGACG
CGGTCATCGGCGGGCTGGGCACCGGGGAAACGACCCTGGCTGATGAACTGATCGACCGGCTGGATCCAGGCTCAGTACTGGTCGCTGATCGGGGGTTTTA
TAGTTTCCACCGGTGGGCTACCGCCCAGGACCGTGGTGCGGATCTGGTGTGGCGGATCCGGAACAATGTGAAACCACACCATGTGCGAACCCTGGAGGAT
GGAACATGGTTGGCCACGATTACCCCGAGCAGTGGGCCGGGGTGGCGGCAGGTTGCCCCAAGGATGGTGCGGGTGGTGGACTACCACATCGATACCGGTG
ACGATGAGGCTGGCATTGTCGACCAGCAGCATCGGTTGTTGACCACCTTGTTGGATCCTGATGAGGTGTCGGTGGAGGATCTGATCGCGGCGTATCACGC
CCGGTGGGAGATCGAGAACACTTTCGATGAGTTGAAAACCCATCAGCGTGGACGAGAAAGGGTGTTGCGGTCAAAGTCCCCGGAGTTGGTGCGTCAGGAG
ATCTGGGGGCATCTGTGCTGCCACTACGCGATCAGGACACTGATGGCTGATGCGGCCACTGCCAGTGAGCACCCTCCGGTGCGGGTGTCATTTGTTAAGG
CCTTGAAGCTTACCCGTCGTTCGGTGATCCAGGGCTCTTTTCCCCCCTGACGACCCGCACACCGCCCGAAGTTTATGGGATCGGGCAGTGTCTTGGCTGG
CCAGGTACCTCAACCCACCGCGACGAAGGCGGAAGAATCCGCGGGTGATTAAACGAAAGATGTCGAAATGGCCGGTGAAACGATCCCATCATTGTGGTCG
GCCACTCGGCCCAGTGGTCACCGTCATCCCACCACTAACTTAACGGTATTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1212 bp | 404 aa | 36 | 1247 | + | No |
Chemistry : DDE
ORF sequence :
MPRHGRTQPDTDRRLSDLISVGLLTRVFPADVVDEVVAQAGRTQQRSRILPSRVMVYYTITMALNSDGSYEDIYADLTDGLSWACRWEEQAVPAPSRSAI
FQARVRLGHEPMAALFARVAQPLARPGWEGAWLAGKRLLAVDGTTLDVPDTPANVEHFGRPGTGRGQAGYPQARVVAIAECGSHAILDAVIGGLGTGETT
LADELIDRLDPGSVLVADRGFYSFHRWATAQDRGADLVWRIRNNVKPHHVRTLEDGTWLATITPSSGPGWRQVAPRMVRVVDYHIDTGDDEAGIVDQQHR
LLTTLLDPDEVSVEDLIAAYHARWEIENTFDELKTHQRGRERVLRSKSPELVRQEIWGHLCCHYAIRTLMADAATASEHPPVRVSFVKALKLTRRSVIQG
SFPP
FQARVRLGHEPMAALFARVAQPLARPGWEGAWLAGKRLLAVDGTTLDVPDTPANVEHFGRPGTGRGQAGYPQARVVAIAECGSHAILDAVIGGLGTGETT
LADELIDRLDPGSVLVADRGFYSFHRWATAQDRGADLVWRIRNNVKPHHVRTLEDGTWLATITPSSGPGWRQVAPRMVRVVDYHIDTGDDEAGIVDQQHR
LLTTLLDPDEVSVEDLIAAYHARWEIENTFDELKTHQRGRERVLRSKSPELVRQEIWGHLCCHYAIRTLMADAATASEHPPVRVSFVKALKLTRRSVIQG
SFPP
Blast result :
Comments
ISCef4 is 43% aa similar to IS4.
ISCef4 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-75-D(N3)-108-E(C1). The copy number in Corynebacterium efficiens YS-314 is 7.
ISCef4 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-75-D(N3)-108-E(C1). The copy number in Corynebacterium efficiens YS-314 is 7.
References
1] Nishio,Y., Nakamura,Y., Kawarabayasi,Y., Usuda,Y., Kimura,E., Sugimoto,S., Matsui,K., Yamagishi,A., Kikuchi,H., Ikeo,K. and Gojobori,T.(2003)Genome Res. 13(7), 1572-1579.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18