ISAzvi6
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001157 | ND | Azotobacter vinelandii | Azotobacter vinelandii AvOP |
DNA section
IS Length : 1422 bp
Ends
IR Length : 19
IRL : TAATGCTGTTCACTTAAGCATTTGAGTCCAGGCCGGGCTTTCTAGAAAAT
IRR : TAATGCTGTTCACTTAAGCGGAGCGGCCTTACGATCCACTGGATATCGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACACCAGGC | CG | CTCGAAGCGG | 2 |
DNA sequence
TAATGCTGTTCACTTAAGCATTTGAGTCCAGGCCGGGCTTTCTAGAAAATCCGATACCGGCCCTCTGGTATCGGATTTTTTATACCCATTCAGCAGCAGT
TGCTCGACCTCGGCGATCTCTTCAATTTCTCCGACCTCAGCACCTTCACCCGGAACATTCCTGTTGAATGGGTCGCCAGTGCGCTCAATCTCTCTGCACA
AGCCACCATACGGCGTCGCCGCCTGCCGAGCGATCAGGTACTTTGGTTGGTGCTGGGCATGGCCTTGTTCCGCGATGAGCCTGTGCATGAGGTGGCCAGA
CGCCTGAATATCTGTGCTCAAGGCCTGGCCTCCGATCAACTGCTGGCGCGCAGCGGCGTGACTGAAGCGCGTAAGCGACTAGGAGCCGATCCAGTCGAAT
CCTTGTTCCGCCAGACAGGTAAACAATGGGGTGGCGAGCGATACGAAGGCGATGATTGGAACGGTTTGCAAGTGTTTGCGGTGGATGGCGCATTGCTGCG
AACCCCTGATTCACCTGAGCTGCGAGAGCATTTTGGCTCTGGTAACACGGGGACCGAGCGCCAGACGCCTTTTCCAATGCGGCGTCTCGTTGCCCTGATG
AATGTGCGTTCTCACGTGATCCCCGATGCCCAGCTCAGCCCCTATCGCGGCAGCGAGATGCGCTTGGCCGAAACCTTCCTCGGCCAGATCCCGGACAATT
CGATCACGTTGTTCGACAAAGGCTTTTGGGGCGCTGATCTGCTGTCAAGCGTCGCCGAGGGTGGGAGCAGTCGTCACGGGTTGACGCCGGCTCGCAAGAA
TCTGGTCATAGAGGAAATCGAGCGTTGTGGCGAGCACGATCGCCGTGTTCGGATGAAGGTCTCGCCCCAGGCACGCAAACGAAACCCGAATCTGCCCACG
CATTGGGATGTGCGTGAGGTCAGTTATGAAAGCCAAGGGCACGCCAGATCGCTGCTCACTTCACTACCCGTAGAAAGCTACAGCGCAGGCGCTGTCGCCG
CGTTGTACCTGGAACGCTGGGAGATCGAGTTGGGCTTCCGGGACATCAAAAGCTCGATGCAACAGAACGCGGTGACGCTGCGTAGCAAGAAGGTCGAGCT
GATTTATCAGGAAGTCTGGGGGCTCTTGCTGGCTTACAACATCATTCGCCGCGAGGCCAGCCAAGCCGCTGTGGCCTTTGGGCGGGCGCCCTCGGACATA
CGCTTCAAACCCGCCTGTCAGTACATCGCCGTGCAATTGATCGTCATGGCGGCGGCCAATCCGGTATCGGCAACGGGCAGGAGGTTGGCTGAGCTGCGAG
GAGGTATCGGGGGACTGTTTCTGGATCACCGTCCAAGGCCAGGAAGGCCAAGGACGGTGAAGATTTCCAAAACCCGATATCCAGTGGATCGTAAGGCCGC
TCCGCTTAAGTGAACAGCATTA
TGCTCGACCTCGGCGATCTCTTCAATTTCTCCGACCTCAGCACCTTCACCCGGAACATTCCTGTTGAATGGGTCGCCAGTGCGCTCAATCTCTCTGCACA
AGCCACCATACGGCGTCGCCGCCTGCCGAGCGATCAGGTACTTTGGTTGGTGCTGGGCATGGCCTTGTTCCGCGATGAGCCTGTGCATGAGGTGGCCAGA
CGCCTGAATATCTGTGCTCAAGGCCTGGCCTCCGATCAACTGCTGGCGCGCAGCGGCGTGACTGAAGCGCGTAAGCGACTAGGAGCCGATCCAGTCGAAT
CCTTGTTCCGCCAGACAGGTAAACAATGGGGTGGCGAGCGATACGAAGGCGATGATTGGAACGGTTTGCAAGTGTTTGCGGTGGATGGCGCATTGCTGCG
AACCCCTGATTCACCTGAGCTGCGAGAGCATTTTGGCTCTGGTAACACGGGGACCGAGCGCCAGACGCCTTTTCCAATGCGGCGTCTCGTTGCCCTGATG
AATGTGCGTTCTCACGTGATCCCCGATGCCCAGCTCAGCCCCTATCGCGGCAGCGAGATGCGCTTGGCCGAAACCTTCCTCGGCCAGATCCCGGACAATT
CGATCACGTTGTTCGACAAAGGCTTTTGGGGCGCTGATCTGCTGTCAAGCGTCGCCGAGGGTGGGAGCAGTCGTCACGGGTTGACGCCGGCTCGCAAGAA
TCTGGTCATAGAGGAAATCGAGCGTTGTGGCGAGCACGATCGCCGTGTTCGGATGAAGGTCTCGCCCCAGGCACGCAAACGAAACCCGAATCTGCCCACG
CATTGGGATGTGCGTGAGGTCAGTTATGAAAGCCAAGGGCACGCCAGATCGCTGCTCACTTCACTACCCGTAGAAAGCTACAGCGCAGGCGCTGTCGCCG
CGTTGTACCTGGAACGCTGGGAGATCGAGTTGGGCTTCCGGGACATCAAAAGCTCGATGCAACAGAACGCGGTGACGCTGCGTAGCAAGAAGGTCGAGCT
GATTTATCAGGAAGTCTGGGGGCTCTTGCTGGCTTACAACATCATTCGCCGCGAGGCCAGCCAAGCCGCTGTGGCCTTTGGGCGGGCGCCCTCGGACATA
CGCTTCAAACCCGCCTGTCAGTACATCGCCGTGCAATTGATCGTCATGGCGGCGGCCAATCCGGTATCGGCAACGGGCAGGAGGTTGGCTGAGCTGCGAG
GAGGTATCGGGGGACTGTTTCTGGATCACCGTCCAAGGCCAGGAAGGCCAAGGACGGTGAAGATTTCCAAAACCCGATATCCAGTGGATCGTAAGGCCGC
TCCGCTTAAGTGAACAGCATTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1323 bp | 441 aa | 88 | 1410 | + | No |
Chemistry : DDE
ORF sequence :
IQQQLLDLGDLFNFSDLSTFTRNIPVEWVASALNLSAQATIRRRRLPSDQVLWLVLGMALFRDEPVHEVARRLNICAQGLASDQLLARSGVTEARKRLGA
DPVESLFRQTGKQWGGERYEGDDWNGLQVFAVDGALLRTPDSPELREHFGSGNTGTERQTPFPMRRLVALMNVRSHVIPDAQLSPYRGSEMRLAETFLGQ
IPDNSITLFDKGFWGADLLSSVAEGGSSRHGLTPARKNLVIEEIERCGEHDRRVRMKVSPQARKRNPNLPTHWDVREVSYESQGHARSLLTSLPVESYSA
GAVAALYLERWEIELGFRDIKSSMQQNAVTLRSKKVELIYQEVWGLLLAYNIIRREASQAAVAFGRAPSDIRFKPACQYIAVQLIVMAAANPVSATGRRL
AELRGGIGGLFLDHRPRPGRPRTVKISKTRYPVDRKAAPLK
DPVESLFRQTGKQWGGERYEGDDWNGLQVFAVDGALLRTPDSPELREHFGSGNTGTERQTPFPMRRLVALMNVRSHVIPDAQLSPYRGSEMRLAETFLGQ
IPDNSITLFDKGFWGADLLSSVAEGGSSRHGLTPARKNLVIEEIERCGEHDRRVRMKVSPQARKRNPNLPTHWDVREVSYESQGHARSLLTSLPVESYSA
GAVAALYLERWEIELGFRDIKSSMQQNAVTLRSKKVELIYQEVWGLLLAYNIIRREASQAAVAFGRAPSDIRFKPACQYIAVQLIVMAAANPVSATGRRL
AELRGGIGGLFLDHRPRPGRPRTVKISKTRYPVDRKAAPLK
Blast result :
Comments
ISAzvi6 is 53% aa similar to IS4.
ISAzvi6 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-103-E(C1).
Genome in progress : Accession number : NZ_AAAU03000027.1
ISAzvi6 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-103-E(C1).
Genome in progress : Accession number : NZ_AAAU03000027.1
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2005)Unpublished
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18