ISPosp4

  • Family IS4
  • Group IS4Sa
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007950 ND Polaromonas sp.
Polaromonas sp. JS666
DNA section
IS Length : 1328 bp

Ends


IR Length : 18

IRL : CAGTAGTGTCCGGTTAAATCCGTCTGGATTCACGTGAAAGCCAATACTGG
IRR : CAGTAGTGTCCGGTTAAAAGTTGAACAGATTCAACTGGTTATCACCAAGC

Insertion site


Left flankDirect repeatRight flankDR Length
TCGCATCCGCAGATTCGATGGGAAATGAC9

DNA sequence

CAGTAGTGTCCGGTTAAATCCGTCTGGATTCACGTGAAAGCCAATACTGGCGCGGCCTGGAGGCCGATTTTGTTTGGTGCCGATTTGAAACTTGATTTCT
CATTTTTGTGTAATTTCCCGGGATTGATGCCCTGGGAGATTGGCCATGAATGTCGGCAAGACGCTGTTTGCGCAGGTCATGGAGTTTGTTCCATGGACGA
GTTTTTCGCGCATCGTGCAGCGCCACGGTGGAGACGCCGGGGTACGCAGGATGAATTGCGCCGAACAGTTTCGCGTCATGGCGTTTGCGCAGTTGACTTG
GCGCGAGTCCTTGCGCGACATCGAGGTGACGCTGGGAGCGAACGCCGGCAAGCTCTATTCGATGGGTTTGCGCCACAGCGTTCATCGATCCACTTTGGCC
GACGCAAACGACTCGCGGGATTGGCGCATTTGGTCCGACCTCGCGGCCTTGCTCATTCGACGAGCCCGCAAGCTCTACCGCGAAGAGGACTTGGGGCTGG
ACTTGACCAATACGGTTTACGCCCTGGATGCAACGACGATCGATCTGTGTTTGAGCCTGTTCGACTGGGCCCCGTTTCGCAGCACCAAGGCAGCCGTGAA
GATGCACACGTTGCTGGACTTGCGCGGCTCCATTCCCGCCTTCATCCACATCAGCGACGGCAAGATGGGCGATGTCAACGTGCTGGACTTCCTGCCCGTC
GAGGCGGGCGCCTTCTACGTGATGGATCGTGGCTATCTGGACTTCGCTCGCCTGTACAAGATGCATCAGGCCGGCGCCTTCTTCGTCACACGCGCCAAGC
GCGGCATGAACGCCAGGCGGGTGTACTCGGCTCAGACCGACAGGGCGACTGGCGTGATCTGCGATCAGAGCATTGCGATGAATGGCTTCTACGTCTGCAA
GGACTACCCCGAGCAGTTGCGCCGCATTCGCTTCAAAGACCCCGAGACGGGCAAGACGCTGGTGTTCCTGACCAACAACACGACCCTGCCAGCGCTGACC
ATCGCCGCTTTGTACAAGAGCCGCTGGCAGGTGGAGTTGTTCTTCAAGTGGATCAAGCAGCATTTGCGGATCAAGAAATTTCTGGGCACGAGCGAGAACG
CCGTGAAAACGCAAATCTGGTGCGCCGTCTGCACCTACGTGCTCATCGCCATCGTCAAGAAGGAGCTTCAACTCGATGCCTCGCTCTACACATTGCTACA
GATTCTTTCCGTCTCGATCTTCGAGAAAACTCAGCTCTCATGCGCCCTGCAGCTCGGTTTCCCAATTCCAGAAACACCGCTTGGTGATAACCAGTTGAAT
CTGTTCAACTTTTAACCGGACACTACTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1167 bp389 aa1461312+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNVGKTLFAQVMEFVPWTSFSRIVQRHGGDAGVRRMNCAEQFRVMAFAQLTWRESLRDIEVTLGANAGKLYSMGLRHSVHRSTLADANDSRDWRIWSDLA
ALLIRRARKLYREEDLGLDLTNTVYALDATTIDLCLSLFDWAPFRSTKAAVKMHTLLDLRGSIPAFIHISDGKMGDVNVLDFLPVEAGAFYVMDRGYLDF
ARLYKMHQAGAFFVTRAKRGMNARRVYSAQTDRATGVICDQSIAMNGFYVCKDYPEQLRRIRFKDPETGKTLVFLTNNTTLPALTIAALYKSRWQVELFF
KWIKQHLRIKKFLGTSENAVKTQIWCAVCTYVLIAIVKKELQLDASLYTLLQILSVSIFEKTQLSCALQLGFPIPETPLGDNQLNLFNF

 

Blast result :
Comments
ISPosp4 is 80% aa similar to ISDar2. ISPosp4 was found by screening completely sequenced genomes for sequences homologous to the ISRso13 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-102-E(C1). The copy number in the Polaromonas sp. JS666 genome is 1. ISPosp4 is located on plasmid 2.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Munk,A.C., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Anderson,I. and Richardson,P. (2006) Unpublished