ISfinder Mirror Site

ISDpr1

Family IS4
Group IS4Sa

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	delta proteobacterium	delta proteobacterium MLMS-1

DNA section

IS Length : 1325 bp

Ends

IR Length : 15

IRL : CACTAGTGTCCGGTTAGGGTCGCTACGTTGCTTAGCAACCATTTAATAAT
IRR : CACTAGTGTCCGGTTGTTAATTGAATAAATTCAACTGTTTACAGGATGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CATCCCAACT		ATGTTGACTA	0

DNA sequence

CACTAGTGTCCGGTTAGGGTCGCTACGTTGCTTAGCAACCATTTAATAATTCGTCAAAAAACTATTGTCATGCAAGAAATCGACTTGAATTTTACCAACC
ACCTCCGGCATTCTCACGGCACCACTTTAAAGGAGGGTGCCCATGTATACCGGAAAGCTGGTTTTCTCCCAGGTGATGGATTTCCTGCCGTTGCCTGTTT
TTCGCAATTGCGTGGCCAAATACCGAGGCCATCACAAAGTCAGAAAATTTTCCTGTCTCGATCAATACTACTGCATGGCCTTTGCCCAAATCACTTACCG
GGAGAGCCTGCGGGACATCGAAGCCTGCCTGCGTTCCCAGGGCAGCAAACTCTACCACATGGGCATTCGCGGCAGAGTCAGCCGCTCAACCATGGCCGAT
GCCAACGAGCAGCGGGATTGGCGCATTTATGCCGAACTGGCCCACTCCCTGATTGTTACGGCCCGCGAGCTGTATCAGGCCGAGACTTTTCTGACCGACT
TGAACGAGACCGCCTACGCCCTCGATTCGTCAACCATCGATCTCTGCCTGTCCCTGTTCCCGTGGGCTGCTTTTCGCAAAACCAAAGCAGCCGTCAAATT
ACATACTTTGCTCGACCTACGGGGCAACCTGCCAGCCTTTATCCATATTTCAGACGGCAAGCTGCACGATGTCAACGTTCTCGATCTTCTGCCGCTGGAG
CCTGGCGCATTTTACATCATGGATCGGGCCTATCTGGATTTCGCCAGGCTCTACCACATGAGCAAGATGGGTGCCTTTTTCGTAACCAGGGCCAAATCGA
ACACCAAATTCGAGCGGCGCTATTCGCATCCGGTGGACCGCACAACCGGCGTAATCTGCGATCAGACCATTGTCTTGACGGGGGTCAGATCACGCCTCGA
CTACCCCGAAAAGCTACGGCGAATCAAATTCCGCGATACCGACACCGGCAACACCTTGGATTTCTTGACCAACAATTTCGCTTTGCCGGCAACGACCATA
GCCGATTTGTACCGTTGTCGCTGGCAAGTGGAGCTGTTCTTCAAGTGGATCAAGCAAAACCTCAGAATAAAAAGGTTTTACGGGACATCCGAAAACGCGG
TGAAATCCCAAATCTGGATCGCGGTTTCGGTCTATGTCATTGCGGCCATCATGAAAAAACAGCTCCATCTCCAGGACAGTCTCTACACTTTGCTCCAAGT
TTTGAGCGTTACCATTTTCGAGCGAACACCGATATTTCAGCTACTTACCGAAAGAGATTACAAAACTGCACAGGAGCCATCCTGTAAACAGTTGAATTTA
TTCAATTAACAACCGGACACTAGTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1164 bp	388 aa	143	1306	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYTGKLVFSQVMDFLPLPVFRNCVAKYRGHHKVRKFSCLDQYYCMAFAQITYRESLRDIEACLRSQGSKLYHMGIRGRVSRSTMADANEQRDWRIYAELA
HSLIVTARELYQAETFLTDLNETAYALDSSTIDLCLSLFPWAAFRKTKAAVKLHTLLDLRGNLPAFIHISDGKLHDVNVLDLLPLEPGAFYIMDRAYLDF
ARLYHMSKMGAFFVTRAKSNTKFERRYSHPVDRTTGVICDQTIVLTGVRSRLDYPEKLRRIKFRDTDTGNTLDFLTNNFALPATTIADLYRCRWQVELFF
KWIKQNLRIKRFYGTSENAVKSQIWIAVSVYVIAAIMKKQLHLQDSLYTLLQVLSVTIFERTPIFQLLTERDYKTAQEPSCKQLNLFN

Blast result :

Comments

ISDpr1 is 76% aa similar to ISDar2. ISDpr1 was found by screening completely sequenced genomes for sequences homologous to the ISRso13 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-102-E(C1). The sequencing of the delta proteobacterium MLMS-1 chromosome is currently in progress. Accession number: NZ_AAQF00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P.(2006)Unpublished.