ISDar2
- Family IS4
- Group IS4Sa
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007298 | ND | Dechloromonas aromatica | Dechloromonas aromatica RCB |
DNA section
IS Length : 1322 bp
Ends
IR Length : 19/19
IRL : CACTGCTGTCCGGTTAAATGAAGGGTCGAAACCCTGAAGCCCAAGACTGA
IRR : CACTGCTGTCCGGTTAAATCGTGAATAGATTCAATTGGTTGTTGTGCGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGACATACCG | ATTGCACAT | ACCGTAAGTT | 9 |
DNA sequence
CACTGCTGTCCGGTTAAATGAAGGGTCGAAACCCTGAAGCCCAAGACTGATGCGGGTTTCCGAGCGATGAAGGGTGGTGTCGATTTGAACGGCGATTACC
GATTCTGGCAATCTTCCGGGGTTTTGCCCGGAGGCCGCCATGAATATCGGCAAGACGCTGTTCGCACAACTCATGGACTTTCTGCCATGGACGACCTTCG
CGCGGTACGTTGCGCGTTACGAAGGCGACAAGGGAGTTCGCACGCTGACGTGCGCGGAACAGTTCCGAATCATGGCCTTCGCGCAACTGACCTACCGCGA
GAGCTTGCGCGACATCGAAGTCTGCCTGTCGGCGCAGGCTGGCAAGCTCTACCCCATGGGATTCCGGCAGGAGATCAAGCGATCTACGCTGGCCGATGCC
AACGAGACACGGGATTGGCGTATCTACGCCGAGTTTGCCCAGCGCCTGATCGAGCAAGCGCGCAAGCTCTATGCGGGCGACAGCTTTGGCATCGAGTTGG
AGAACACGACGTACGCGCTGGACTCGACGACCATCGATCTGTGCCTGTCGCTGTTTCCTTGGGCGTTGTTTCGCACCACCAAGTCGGCGGTGAAGATGCA
CACGCTGCTCGATCTTCGCGGCAACATTCCCAGCTTCATCCACATCTCCGACGGCAAGCTGGGCGATGTCAATGTGCTCGACATTCTGGTGCTGGAGGTT
GGGGCAATCTACGTCATGGATCGGGGCTACTTGGACTTCGCCCGGCTTTATCTGATGCATCAGGCGCAAGCCTTCTTCGTTACCCGTACCAAGTCGAACA
CCCGATTGCGGCGAGTCCATTCGGCGGCGGTAGATCGCAGCACCGGCATCATCTGCGATCAGACCATTGCACTTACCGGCACGACCAGTCGCCAGGACTA
CCCAGAACATCTCCGGCGCATCCGATTCAAAGATCCCGAGACTGACAAGACGCTGGTGTTCCTCACCAACAACTTCACCATTCCAGCAGCCAACATCTGC
GCGCTCTACAAGCGCCGCTGGCAAGTGGAACTGTTCTTCAAGTGGATCAAGCAGCATCTGCGGATCAAAAAGTTCTACGGCAATTCAGACAATGCCGTCA
GGTCGCAAATCTGGATCGCCATTTCGGTCTATGTTCTCGTCGCCATCGTCAAAAAACGCCTCAAGCTCGACGCCTCGCTCTACACTTTGCTACAGATTTT
GTCGCTCACCCTTTTCGAGAAAATGCCCTTGCAGCAAGCCTTTTCACCCAGCGAGTACATTCTTCCAGAGGGCGCGCACAACAACCAATTGAATCTATTC
ACGATTTAACCGGACAGCAGTG
GATTCTGGCAATCTTCCGGGGTTTTGCCCGGAGGCCGCCATGAATATCGGCAAGACGCTGTTCGCACAACTCATGGACTTTCTGCCATGGACGACCTTCG
CGCGGTACGTTGCGCGTTACGAAGGCGACAAGGGAGTTCGCACGCTGACGTGCGCGGAACAGTTCCGAATCATGGCCTTCGCGCAACTGACCTACCGCGA
GAGCTTGCGCGACATCGAAGTCTGCCTGTCGGCGCAGGCTGGCAAGCTCTACCCCATGGGATTCCGGCAGGAGATCAAGCGATCTACGCTGGCCGATGCC
AACGAGACACGGGATTGGCGTATCTACGCCGAGTTTGCCCAGCGCCTGATCGAGCAAGCGCGCAAGCTCTATGCGGGCGACAGCTTTGGCATCGAGTTGG
AGAACACGACGTACGCGCTGGACTCGACGACCATCGATCTGTGCCTGTCGCTGTTTCCTTGGGCGTTGTTTCGCACCACCAAGTCGGCGGTGAAGATGCA
CACGCTGCTCGATCTTCGCGGCAACATTCCCAGCTTCATCCACATCTCCGACGGCAAGCTGGGCGATGTCAATGTGCTCGACATTCTGGTGCTGGAGGTT
GGGGCAATCTACGTCATGGATCGGGGCTACTTGGACTTCGCCCGGCTTTATCTGATGCATCAGGCGCAAGCCTTCTTCGTTACCCGTACCAAGTCGAACA
CCCGATTGCGGCGAGTCCATTCGGCGGCGGTAGATCGCAGCACCGGCATCATCTGCGATCAGACCATTGCACTTACCGGCACGACCAGTCGCCAGGACTA
CCCAGAACATCTCCGGCGCATCCGATTCAAAGATCCCGAGACTGACAAGACGCTGGTGTTCCTCACCAACAACTTCACCATTCCAGCAGCCAACATCTGC
GCGCTCTACAAGCGCCGCTGGCAAGTGGAACTGTTCTTCAAGTGGATCAAGCAGCATCTGCGGATCAAAAAGTTCTACGGCAATTCAGACAATGCCGTCA
GGTCGCAAATCTGGATCGCCATTTCGGTCTATGTTCTCGTCGCCATCGTCAAAAAACGCCTCAAGCTCGACGCCTCGCTCTACACTTTGCTACAGATTTT
GTCGCTCACCCTTTTCGAGAAAATGCCCTTGCAGCAAGCCTTTTCACCCAGCGAGTACATTCTTCCAGAGGGCGCGCACAACAACCAATTGAATCTATTC
ACGATTTAACCGGACAGCAGTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 389 aa | 140 | 1306 | + | No |
Chemistry : DDE
ORF sequence :
MNIGKTLFAQLMDFLPWTTFARYVARYEGDKGVRTLTCAEQFRIMAFAQLTYRESLRDIEVCLSAQAGKLYPMGFRQEIKRSTLADANETRDWRIYAEFA
QRLIEQARKLYAGDSFGIELENTTYALDSTTIDLCLSLFPWALFRTTKSAVKMHTLLDLRGNIPSFIHISDGKLGDVNVLDILVLEVGAIYVMDRGYLDF
ARLYLMHQAQAFFVTRTKSNTRLRRVHSAAVDRSTGIICDQTIALTGTTSRQDYPEHLRRIRFKDPETDKTLVFLTNNFTIPAANICALYKRRWQVELFF
KWIKQHLRIKKFYGNSDNAVRSQIWIAISVYVLVAIVKKRLKLDASLYTLLQILSLTLFEKMPLQQAFSPSEYILPEGAHNNQLNLFTI
QRLIEQARKLYAGDSFGIELENTTYALDSTTIDLCLSLFPWALFRTTKSAVKMHTLLDLRGNIPSFIHISDGKLGDVNVLDILVLEVGAIYVMDRGYLDF
ARLYLMHQAQAFFVTRTKSNTRLRRVHSAAVDRSTGIICDQTIALTGTTSRQDYPEHLRRIRFKDPETDKTLVFLTNNFTIPAANICALYKRRWQVELFF
KWIKQHLRIKKFYGNSDNAVRSQIWIAISVYVLVAIVKKRLKLDASLYTLLQILSLTLFEKMPLQQAFSPSEYILPEGAHNNQLNLFTI
Blast result :
Comments
ISDar2 is 55% aa similar to ISGsu1.
ISDar2 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-62-D(N3)-102-E(C1). The copy number in Dechloromonas aromatica RCB is 1.
ISDar2 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-62-D(N3)-102-E(C1). The copy number in Dechloromonas aromatica RCB is 1.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Trong,S., Kellar,K., Schmutz,J., Larimer,F., Land,M., Ivanova,N. and Richardson,P.(2005)Unpublished
[2]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
[2]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18