ISApu2
- Family IS4
- Group ISH8
Isoform Synonym(s) ISUnCu5
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ698325 | ND | Aeromonas punctata | Uncultured bacterium plasmid pRSB101 Aeromonas punctata (Aeromonas caviae) HGB5 plasmid pFBAOT6 |
DNA section
IS Length : 1388 bp
Ends
IR Length : 19/24
IRL : CATGTACAGGATCTTTAGCTACAACCAATTGAAATAGATAGAAAAAAGGC
IRR : CATGTACAGAACCTTAAGCGGCAAGCGAATTAATAACACCGTCTAACGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATAGCCCTT | GATGGCTAAT | AACATCATGT | 10 |
| AGTGACCTGT | ATCGCCGGTT | 0 |
DNA sequence
CATGTACAGGATCTTTAGCTACAACCAATTGAAATAGATAGAAAAAAGGCTTCGTTTGTGATCTGATTTAATCGCAAAAAAAACCGACACGCCCGAAGCC
ATGACTATTATCAATGCAACCCAGTACCTCAAGCAACTTCTTAGCTCTTCTGAACTCAACCGGATCGGTAAATTTACCGGTTTTTGTCAGCGACTCCGGG
ATATCCAGCCTGCGAGACTACTCCCGGCTCTGCTCTCAGGGCTTGGTTGCGACAAGGTGGATGGTATTGCTGGTTTGCATCGCCATTTCAATGCGTTGCA
GCTCCATGATACTGACCAAATAGCGTATAAACCCTTTCACAACCAGTTACGCAAGCAGGGTTTTCCCTTGTTCATGCGCGCACTGGTTGAACGAGCCATC
GCCTTACGCCTCAAGGAGTGTCTACCGGACGCCCACGGACTGGCAGGCTTTGAGCAGGTACTGCTGCAGGACGGGTCGTCATTCGCGTTACATCCGCAAT
TGGCAGAACATTTCCCGGGCCGATTCAATAAGCACAGCCCCGCTGCGGTAGAGTGTCATATGACCATGTCCTTGCTGGACCAGTCGCCGATATCGATGAG
TATCACGGCGGATACCGAATCGGAGCGCAAGCATCTTCCGGCCGCAAATTCGTTAAATAACAAACTGTTATTGGCGGATGCCGGTTACATCAGCCGCGAG
TACTTTGCTGATGTGACCAAAGCCAAAGGAAGCTATCTGGTCAGAGGGAGTCAAAATCTCAATCCCAAAGTACAGGCAGCTTACCGACAGGACGGCAGGG
AAATGCCCAAGTTGCTCGGAAAAAAGCTCAAGGATATCGATCGGAGAAGCTGCAGAGCCGAGGTTCTGGATCTGGATGTGAGTTCGGGAAAGTATAAATA
TCGACTCATAAGACGTTGGTTTGCAGAGGAAAAACGGTTCTGTATATGGATGACCAATCTTCCGCGTGAGCGATGGCCAGCCGAGAAGGTGATGGCATTG
TATCGTTGCCGTTGGCAGATCGAACTGTTGTTCAAAGAGCTGAAATCACATAACCGGTTGAAGGCGTTCGCGACACGACAAAAAGCGATAGCGGAAGGCT
TGATCTGGGCTAGTTTGCTGGCCTTGCTGGTCAAACGTCGGCTGGCGCTGACGCTGATTGGCAGCGCGGAGTTATCGATGCTGAAGATTGCCAAAAACAG
CGCGCTTTGGTTAATGCCGATATTGGCGAGCATCATCCATGGTGCGTGGCAGGAAATAACAGAGAAGTTGGAGTGGGCAATGGTGTATTTATCCAAAAAC
GGCAAAAAGAGTAGGCAGAGAAAATCCAAGCAAAACAAAACGTTAGACGGTGTTATTAATTCGCTTGCCGCTTAAGGTTCTGTACATG
ATGACTATTATCAATGCAACCCAGTACCTCAAGCAACTTCTTAGCTCTTCTGAACTCAACCGGATCGGTAAATTTACCGGTTTTTGTCAGCGACTCCGGG
ATATCCAGCCTGCGAGACTACTCCCGGCTCTGCTCTCAGGGCTTGGTTGCGACAAGGTGGATGGTATTGCTGGTTTGCATCGCCATTTCAATGCGTTGCA
GCTCCATGATACTGACCAAATAGCGTATAAACCCTTTCACAACCAGTTACGCAAGCAGGGTTTTCCCTTGTTCATGCGCGCACTGGTTGAACGAGCCATC
GCCTTACGCCTCAAGGAGTGTCTACCGGACGCCCACGGACTGGCAGGCTTTGAGCAGGTACTGCTGCAGGACGGGTCGTCATTCGCGTTACATCCGCAAT
TGGCAGAACATTTCCCGGGCCGATTCAATAAGCACAGCCCCGCTGCGGTAGAGTGTCATATGACCATGTCCTTGCTGGACCAGTCGCCGATATCGATGAG
TATCACGGCGGATACCGAATCGGAGCGCAAGCATCTTCCGGCCGCAAATTCGTTAAATAACAAACTGTTATTGGCGGATGCCGGTTACATCAGCCGCGAG
TACTTTGCTGATGTGACCAAAGCCAAAGGAAGCTATCTGGTCAGAGGGAGTCAAAATCTCAATCCCAAAGTACAGGCAGCTTACCGACAGGACGGCAGGG
AAATGCCCAAGTTGCTCGGAAAAAAGCTCAAGGATATCGATCGGAGAAGCTGCAGAGCCGAGGTTCTGGATCTGGATGTGAGTTCGGGAAAGTATAAATA
TCGACTCATAAGACGTTGGTTTGCAGAGGAAAAACGGTTCTGTATATGGATGACCAATCTTCCGCGTGAGCGATGGCCAGCCGAGAAGGTGATGGCATTG
TATCGTTGCCGTTGGCAGATCGAACTGTTGTTCAAAGAGCTGAAATCACATAACCGGTTGAAGGCGTTCGCGACACGACAAAAAGCGATAGCGGAAGGCT
TGATCTGGGCTAGTTTGCTGGCCTTGCTGGTCAAACGTCGGCTGGCGCTGACGCTGATTGGCAGCGCGGAGTTATCGATGCTGAAGATTGCCAAAAACAG
CGCGCTTTGGTTAATGCCGATATTGGCGAGCATCATCCATGGTGCGTGGCAGGAAATAACAGAGAAGTTGGAGTGGGCAATGGTGTATTTATCCAAAAAC
GGCAAAAAGAGTAGGCAGAGAAAATCCAAGCAAAACAAAACGTTAGACGGTGTTATTAATTCGCTTGCCGCTTAAGGTTCTGTACATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1272 bp | 424 aa | 101 | 1372 | + | No |
Chemistry : DDE
ORF sequence :
MTIINATQYLKQLLSSSELNRIGKFTGFCQRLRDIQPARLLPALLSGLGCDKVDGIAGLHRHFNALQLHDTDQIAYKPFHNQLRKQGFPLFMRALVERAI
ALRLKECLPDAHGLAGFEQVLLQDGSSFALHPQLAEHFPGRFNKHSPAAVECHMTMSLLDQSPISMSITADTESERKHLPAANSLNNKLLLADAGYISRE
YFADVTKAKGSYLVRGSQNLNPKVQAAYRQDGREMPKLLGKKLKDIDRRSCRAEVLDLDVSSGKYKYRLIRRWFAEEKRFCIWMTNLPRERWPAEKVMAL
YRCRWQIELLFKELKSHNRLKAFATRQKAIAEGLIWASLLALLVKRRLALTLIGSAELSMLKIAKNSALWLMPILASIIHGAWQEITEKLEWAMVYLSKN
GKKSRQRKSKQNKTLDGVINSLAA
ALRLKECLPDAHGLAGFEQVLLQDGSSFALHPQLAEHFPGRFNKHSPAAVECHMTMSLLDQSPISMSITADTESERKHLPAANSLNNKLLLADAGYISRE
YFADVTKAKGSYLVRGSQNLNPKVQAAYRQDGREMPKLLGKKLKDIDRRSCRAEVLDLDVSSGKYKYRLIRRWFAEEKRFCIWMTNLPRERWPAEKVMAL
YRCRWQIELLFKELKSHNRLKAFATRQKAIAEGLIWASLLALLVKRRLALTLIGSAELSMLKIAKNSALWLMPILASIIHGAWQEITEKLEWAMVYLSKN
GKKSRQRKSKQNKTLDGVINSLAA
Blast result :
Comments
ISApu2 (ISUnCu5) is 78% aa similar to ISApu1. ISUnCu5 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-68-D(N3)-114-E(C1). ISUnCu5 is located on plasmid pRSB101 from an uncultured bacterium and a copy (ISApu2) lies on plasmid pFBAOT6 from Aeromonas punctata (Aeromonas caviae) HGB5.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Szczepanowski,R., Krahn,I., Linke,B., Goesmann,A., Puhler,A. and Schluter,A. (2004) Microbiology (Reading, Engl.) 150 (PT 11), 3613-3630
3] Rhodes,G., Parkhill,J., Bird,C., Ambrose,K., Jones,M.C., Huys,G., Swings,J. and Pickup,R.W. (2004) Appl. Environ. Microbiol. 70 (12), 7497-7510
2] Szczepanowski,R., Krahn,I., Linke,B., Goesmann,A., Puhler,A. and Schluter,A. (2004) Microbiology (Reading, Engl.) 150 (PT 11), 3613-3630
3] Rhodes,G., Parkhill,J., Bird,C., Ambrose,K., Jones,M.C., Huys,G., Swings,J. and Pickup,R.W. (2004) Appl. Environ. Microbiol. 70 (12), 7497-7510