ISAva1
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007413 | ND | Anabaena variabilis | Anabaena variabilis ATCC 29413 |
DNA section
IS Length : 1617 bp
Ends
IR Length : 17
IRL : CATTGGTCACAAGTTAAAAGTCAGGAAATTTTGTCAATACATCTTTGAGC
IRR : CATTGGTCACAAGTTAATGTTGTGAGCCAGTTGTCAAGGGGATTTTAGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTTCTGCTG | CTGTGAACG | GGTCTATACT | 9 |
DNA sequence
CATTGGTCACAAGTTAAAAGTCAGGAAATTTTGTCAATACATCTTTGAGCATAAATATTTCTGTATAAGAATGATAATCGTATCGAAGGGGAAGGGTGCG
AGAGACGCTCGCACCCTTCCACACCCCTATTTTTGATGATCATTATTGTGCTGGTTTGCTAGGATGATTTGCTTGCCAAAAAATTATGTGAAACTCAGTT
AAGACAAATACTTGAGCAATGTAAAAAACCGTGAGTAGCAGCCGTAAGACTAATCGAGATCATGCCAAAAAGAAGCAACGGCCGATGGTGGAAGACGAAG
TAATAGCATCGCATCTGTCGGCATTACTAACACCAGCCATTACATCCCAAGAAAATTACTATCGGCAACTGGGATTAAGAGATAGGATTCTCAACTTACC
ATTAATGGTGGCGGCGGTGCTAACTCTACTCTGGCGTGATGTTGCAGGAGTCACGGAACTGACAAGAATGTTAGCCAGAGAAGGCTTTCTATGGTGTAGA
CCCCTGGAGGTTAGCCAGCAAGCAATATCTCAAAGGTTTTTAACTTTTCCGGCTCAATTATTTGAGAAAGTATTTAAAGATTTATTGCCACATTTACAAG
CTTCTTGGCAAAGAAGAAATCAACGTAAAATCCCCCCAAGTGTTCAATTTACTTTGACGAAATTTGAAAAGATATGGATAGTAGACTGTTCAATCTTAGA
AGCGTTATTTCAGAAGCTTGATAGCTTAAAAGATGCGCCCCAAGGACAATTAGCCGGAAAAATTGGCACGGTCATAAATTTAGTAAATCTTCTACCTGTA
GAAATCTGGTTTTGTGAGAATCCCAGAACTGCTGATACAAAGTTTGAAGCAGATATTTTAAATTTAGTTACACCCCATACGTTGCTGTTACTAGATAGAG
GATTCTATCACTTTAACTTTTGGCTACAATTAATTGCTCAGAATGTAAATTTTATTACTCGGTTGAAGAAGGGAGCAGCAATTCATGTCCAGCAAGTATT
TACAGATAGCTTTGCGCTAAGAGACCGTTTGGTGCGTCTTGGTTCTGGCACAAAGAAAACTACGTTTATTACTTTACGTCTCGTTGAAATTCGTTCAGAT
AAAACATGGCATTCTTATTTAACTAGTGTACTTGACCCAGAAGTTTTACCTCCATACGTTGTAGCTGATTTATACCGCCGCAGATGGCGAATTGAAGATG
CTTTTAATACTGTAAAGCGACTTTTGGGTTTAAGTTATTTATGGACTGGTTCTGTTAATGGTGTTCAATTGCAAGTTTGGGGTACTTGGTTATTTTATGC
TGTATTGGTTGATTTAGGTGATGCTGTCGCTGACGAACTTTCTCTACCTTTTGACTCCATTTCTTTAGAGATGATTTATCGTGGACTTTATCATTTTTAT
GTAGCCCATCAACAAGGCAAGGCAACTGACCCTATTAAATATTTTGCATCTCCTGAAAATCAAGACTTAGGGATTGTTAAACGGCAACGAAAACCAAATA
TGAAGCTCATTGTTGCACCCTTCCCTGATCAACAACGAGGAACTGAGGAATTTTTCTTTCAACCTCCTTCTAAAATCCCCTTGACAACTGGCTCACAACA
TTAACTTGTGACCAATG
AGAGACGCTCGCACCCTTCCACACCCCTATTTTTGATGATCATTATTGTGCTGGTTTGCTAGGATGATTTGCTTGCCAAAAAATTATGTGAAACTCAGTT
AAGACAAATACTTGAGCAATGTAAAAAACCGTGAGTAGCAGCCGTAAGACTAATCGAGATCATGCCAAAAAGAAGCAACGGCCGATGGTGGAAGACGAAG
TAATAGCATCGCATCTGTCGGCATTACTAACACCAGCCATTACATCCCAAGAAAATTACTATCGGCAACTGGGATTAAGAGATAGGATTCTCAACTTACC
ATTAATGGTGGCGGCGGTGCTAACTCTACTCTGGCGTGATGTTGCAGGAGTCACGGAACTGACAAGAATGTTAGCCAGAGAAGGCTTTCTATGGTGTAGA
CCCCTGGAGGTTAGCCAGCAAGCAATATCTCAAAGGTTTTTAACTTTTCCGGCTCAATTATTTGAGAAAGTATTTAAAGATTTATTGCCACATTTACAAG
CTTCTTGGCAAAGAAGAAATCAACGTAAAATCCCCCCAAGTGTTCAATTTACTTTGACGAAATTTGAAAAGATATGGATAGTAGACTGTTCAATCTTAGA
AGCGTTATTTCAGAAGCTTGATAGCTTAAAAGATGCGCCCCAAGGACAATTAGCCGGAAAAATTGGCACGGTCATAAATTTAGTAAATCTTCTACCTGTA
GAAATCTGGTTTTGTGAGAATCCCAGAACTGCTGATACAAAGTTTGAAGCAGATATTTTAAATTTAGTTACACCCCATACGTTGCTGTTACTAGATAGAG
GATTCTATCACTTTAACTTTTGGCTACAATTAATTGCTCAGAATGTAAATTTTATTACTCGGTTGAAGAAGGGAGCAGCAATTCATGTCCAGCAAGTATT
TACAGATAGCTTTGCGCTAAGAGACCGTTTGGTGCGTCTTGGTTCTGGCACAAAGAAAACTACGTTTATTACTTTACGTCTCGTTGAAATTCGTTCAGAT
AAAACATGGCATTCTTATTTAACTAGTGTACTTGACCCAGAAGTTTTACCTCCATACGTTGTAGCTGATTTATACCGCCGCAGATGGCGAATTGAAGATG
CTTTTAATACTGTAAAGCGACTTTTGGGTTTAAGTTATTTATGGACTGGTTCTGTTAATGGTGTTCAATTGCAAGTTTGGGGTACTTGGTTATTTTATGC
TGTATTGGTTGATTTAGGTGATGCTGTCGCTGACGAACTTTCTCTACCTTTTGACTCCATTTCTTTAGAGATGATTTATCGTGGACTTTATCATTTTTAT
GTAGCCCATCAACAAGGCAAGGCAACTGACCCTATTAAATATTTTGCATCTCCTGAAAATCAAGACTTAGGGATTGTTAAACGGCAACGAAAACCAAATA
TGAAGCTCATTGTTGCACCCTTCCCTGATCAACAACGAGGAACTGAGGAATTTTTCTTTCAACCTCCTTCTAAAATCCCCTTGACAACTGGCTCACAACA
TTAACTTGTGACCAATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1317 bp | 439 aa | 285 | 1601 | + | No |
Chemistry : DDE
ORF sequence :
MVEDEVIASHLSALLTPAITSQENYYRQLGLRDRILNLPLMVAAVLTLLWRDVAGVTELTRMLAREGFLWCRPLEVSQQAISQRFLTFPAQLFEKVFKDL
LPHLQASWQRRNQRKIPPSVQFTLTKFEKIWIVDCSILEALFQKLDSLKDAPQGQLAGKIGTVINLVNLLPVEIWFCENPRTADTKFEADILNLVTPHTL
LLLDRGFYHFNFWLQLIAQNVNFITRLKKGAAIHVQQVFTDSFALRDRLVRLGSGTKKTTFITLRLVEIRSDKTWHSYLTSVLDPEVLPPYVVADLYRRR
WRIEDAFNTVKRLLGLSYLWTGSVNGVQLQVWGTWLFYAVLVDLGDAVADELSLPFDSISLEMIYRGLYHFYVAHQQGKATDPIKYFASPENQDLGIVKR
QRKPNMKLIVAPFPDQQRGTEEFFFQPPSKIPLTTGSQH
LPHLQASWQRRNQRKIPPSVQFTLTKFEKIWIVDCSILEALFQKLDSLKDAPQGQLAGKIGTVINLVNLLPVEIWFCENPRTADTKFEADILNLVTPHTL
LLLDRGFYHFNFWLQLIAQNVNFITRLKKGAAIHVQQVFTDSFALRDRLVRLGSGTKKTTFITLRLVEIRSDKTWHSYLTSVLDPEVLPPYVVADLYRRR
WRIEDAFNTVKRLLGLSYLWTGSVNGVQLQVWGTWLFYAVLVDLGDAVADELSLPFDSISLEMIYRGLYHFYVAHQQGKATDPIKYFASPENQDLGIVKR
QRKPNMKLIVAPFPDQQRGTEEFFFQPPSKIPLTTGSQH
Blast result :
Comments
ISAva1 is 45% aa similar to ISGsu1.
ISAva1 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-69-D(N3)-99-E(C1). The copy number in Anabaena variabilis ATCC 29413 is 1.
ISAva1 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-69-D(N3)-99-E(C1). The copy number in Anabaena variabilis ATCC 29413 is 1.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K. and Richardson,P.(2005)Unpublished
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18