ISfinder Mirror Site

ISApu1

Family IS4
Group ISH8

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CR376602	ND	Aeromonas punctata	Aeromonas media WS Aeromonas punctata (Aeromonas caviae) HGB5 plasmid pFBAOT6 Klebsiella pneumoniae plasmid pFOX-7a Pseudomonas aeruginosa ST308 plasmid pCOL-1

DNA section

IS Length : 1384 bp

Ends

IR Length : 20/22

IRL : CATGTACAGGATCTTTAGCGGCAACCAATTGAAATAGATATAAAAAAGGC
IRR : CATGTACAGGACCTTAAGCGGCGAGTCCATTTAAAACGCCTTCCAAGGTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACCCTCACT		AACCGGCGAT	0

DNA sequence

CATGTACAGGATCTTTAGCGGCAACCAATTGAAATAGATATAAAAAAGGCTTCGTTTGTGATCTGATTTTATCGCCAAACAAAATCAACACGCGCGAAGC
CTATGACTATTATCAACGCTAATTACTATCTGGAGCAACTCTTGGCTCCCGCCGCACTCGAGCGCATTGCTCGGCTCTGCAAGTTTTGTCTGCGTCAGCG
TGCCATTACCCCAGCCATGCTCGTACCTGCACTGCTGCGGGCCATGGGAGGCGACCAGGTCAATGACATTGCCGGTTTACACCGCCATTTCAATGCCTTG
CAACTGGTAAACACCCATCAGGTTTCTTACAAACCGTTTCACAATCAGCTGCGCAAAGAGAGCTTTGCCCTGTTTATGAAAGCCCTGGTCGAGCGGGCCA
TCGCCTTGCGTATCGACCATCAACTCAAAGCAGCCTCCCTCGGCGCGTTCAAGCAGGTGTTACTGCACGATGGCACCTCCTTTGCGGTGCATCGGCGCCT
TGCTGCTGATTTTCCCGGCCGGTTCAAGACCATCAGTCCGGCAGCTATCGAGTGCCATATGACCATGTCACTGTCGGAGCAGAGCCCGTTGTGCATGAGT
GTGTCTGCCGATACCGCAGGGGAACGGCAATTTCTGCCTGAAGCCCACACGCTGACCAACTGCCTGCTGCTGGCTGACGCAGGTTACATTGACAGGGCTT
GGTTTGAGCAGGTGAACGACGCTGGCGGATTTTATCTGGTGCGCGGCACGAAAAGCCTGAACCCCAAGATAATCCAAGCCTGGCGCGGTGATGGACGGGA
AGTACCCAAGCTGGCGGGACTATCTCTGAGAGAGGCCGGACGGCGACGCTGTCGGGCCGAGGTACTGGATATGGTGGTGAAGTCCGGCCAGGTCGAGTAC
CGGTTGATCCGGCGCTGGTTTGCTGAGGAAAAGCGGTTCTGTCTGTGGATGACGAACCTACCGCGAGCAGCATGGTCAGCAGAGCAGGTGATGAGCCTGT
ATCGCTGCCGTTGGCAGGTGGAGTTGTTATTTAAAGAGTGGAAATCGCACAACCGGCTCAAGGGGTTTGTGACGGGCGAGAAAGCGATTGCCGAGGGGTT
GGTGTGGACGAGCCTGCTGTCGCTGGTGATGAAGAGACGGGTCGCGCAGAGTGTGATGAGTGGGGCGCTATCGATGCTGAAAGCGTCGAAGAACAGTGCG
ACGTGGTGGCTACCGCTATTGGAAGCAGTGGCCCATAGGGCGCTGACAGAAATAAGGGAAAGATTGGAATGGGCTGCCGATTATTTGGCCAAGAACGCCT
GCCGAACCAAGCAGAGAAAGTCGATACAGAACAGGACCTTGGAAGGCGTTTTAAATGGACTCGCCGCTTAAGGTCCTGTACATG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1266 bp	422 aa	103	1368	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTIINANYYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALLRAMGGDQVNDIAGLHRHFNALQLVNTHQVSYKPFHNQLRKESFALFMKALVERAI
ALRIDHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPLCMSVSADTAGERQFLPEAHTLTNCLLLADAGYIDRAW
FEQVNDAGGFYLVRGTKSLNPKIIQAWRGDGREVPKLAGLSLREAGRRRCRAEVLDMVVKSGQVEYRLIRRWFAEEKRFCLWMTNLPRAAWSAEQVMSLY
RCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWTSLLSLVMKRRVAQSVMSGALSMLKASKNSATWWLPLLEAVAHRALTEIRERLEWAADYLAKNAC
RTKQRKSIQNRTLEGVLNGLAA

Blast result :

Comments

ISApu1 is 52% aa similar to ISPa12. ISApu1 was found by screening completely sequenced genomes for sequences homologous to the ISRm16 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-68-D(N3)-114-E(C1). The copy number on plasmid pFBAOT6 from Aeromonas punctata HGB5 is 1.
File updated the March 3 2015 with the information provided by F. Pfeiffer.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Rhodes,G., Parkhill,J., Bird,C., Ambrose,K., Jones,M.C., Huys,G., Swings,J. and Pickup,R.W. (2004) Appl. Environ. Microbiol. 70 (12), 7497-7510