ISGka3
- Family IS4
- Group IS231
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006510 | ND | Geobacillus kaustophilus | Geobacillus kaustophilus HTA426 |
DNA section
IS Length : 1605 bp
Ends
IR Length : 17/18
IRL : CATAGCCATCAAGTTAAGAAAACAGGAATAAATATATGCCTCATTGGACC
IRR : CATAGCCATCAAGTGAAGCCTTGGAGCGAGTAGCTCCAAGGCTTCACTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTATAATAT | GGGATTGCCTC | AAAGAGTTGT | 11 |
DNA sequence
CATAGCCATCAAGTTAAGAAAACAGGAATAAATATATGCCTCATTGGACCATCCTTTTGAAGACCTCATAATGTCTTAGAAAGGGTGGTTTTTTTGAAGA
CGTCCTTATCTCTCCAAGAAGAATGGCATTGTTTTTTAGACGGATTAAGAAGCGTGTTATCGTGCGAAGAACTTGAACATATGGCCCGAGATCATCAATT
TATTCAACGAAAGGGGAAGTTACGCGCCCATGATTTCGTGGCACTTTGCACATTTCTCCAGGAAGGAGGCGGTCAAAAATCATTGGTGCAGCTTTGCAGT
GCTCTAGCTCTCAAGCAAAACACCTCTCTGAGTGCGGAAGGGCTCAATCAACGGTTTCATGAAAAAGCGGTGAGTTTCCTCAAAGCGGTGTTTGAAAAAC
TGCTCATTCATCAGACTCAGGAGGCCCGTCGCTTATGTCCAAGACATTCGCTGTTTCTCCGGATTCGCATTCTCGATTCCACGTCGTTTCAACTGCCTCC
TGAAATCCAGGGGATTTACGAGGGATGTACGGGGCCTGGGGTGAAAATTCAGCTAGAATATGAATGGCTAGAAGGGAAGGTTCTCCATGTGGACGTCGAA
GATGCCCGTCATCACGACGCCGCCTACGGAGCGTCCCTTCTATCGACCATCCAAGAGGGAGACCTTTGTTTGAAAGACTTAGGCTATTTTTCGCTTGAGG
GATTGCAAGCCATTCATGACGCCGGGGCCTTTTATATTTCACGGCTCAAACACAATGTAGGGATTTACCAAAAAGAAGGGGACCGATTTCGAAAATGGGA
ACCAGAGGATTTCCTTGCGGTGCTGCAACCGGGAGAAACCATGGAACTGGAACATGCGTATGTCAGTGGAAAGAAAGTTCATCAACCGCGGCTCATTGTG
TATCGGCTGACAGAAGAACAGGAGCGACAGAAGGAAGGACAATGGAAACAGAAGGCGAAACAAAAAGGGGCCGCGTACGTGACGCGACGCCCCCACCCAA
TATATGTATATATCACCAATATTCCAGCGATTTATACCTCTTTGCACGAAATTCATACGCTGTATTCTCTTCGCTGGCAGATTGAGGTGGTATTTAAGAC
ATGGAAATCCCTGTTTCACATTCATCGATTCAAACCGATGAAAGGGGCTCGCTTTCAGTGCCACCTGTATGGAACATTGATTGCCTTATTGATCAGCTCC
ACTGTCATGTTTAAAATGCGGGAATGGCTCTATCGAAAACAGAAAAAGGAATTGAGCGAATATAAGGCGATGAGTATGATCAAGGAGTTCGGTATGGATT
TATTTCAAGCCTTATGGTGTTCGGAGGCGTTAGCTGTCCAACTTCTTTTAAAACTGTGTGACATCATCGCTCAACACGGAAAAAAATCAAGGCGTTATAC
AAAAAAGAGCGCTTTAGACATTATCGAGAGTTTGGTCATCATGCCGGTGTCGTAAGAAATTCCCGCCTCATACCATGAAAAAAACCTTCTATCGGGAAGG
TGTATTTGGCATGCCTATCTTTATATATTCTCACGGTCACAGCCTCCAAAGATGAAGAGTGAAGCCTTGGAGCTACTCGCTCCAAGGCTTCACTTGATGG
CTATG
CGTCCTTATCTCTCCAAGAAGAATGGCATTGTTTTTTAGACGGATTAAGAAGCGTGTTATCGTGCGAAGAACTTGAACATATGGCCCGAGATCATCAATT
TATTCAACGAAAGGGGAAGTTACGCGCCCATGATTTCGTGGCACTTTGCACATTTCTCCAGGAAGGAGGCGGTCAAAAATCATTGGTGCAGCTTTGCAGT
GCTCTAGCTCTCAAGCAAAACACCTCTCTGAGTGCGGAAGGGCTCAATCAACGGTTTCATGAAAAAGCGGTGAGTTTCCTCAAAGCGGTGTTTGAAAAAC
TGCTCATTCATCAGACTCAGGAGGCCCGTCGCTTATGTCCAAGACATTCGCTGTTTCTCCGGATTCGCATTCTCGATTCCACGTCGTTTCAACTGCCTCC
TGAAATCCAGGGGATTTACGAGGGATGTACGGGGCCTGGGGTGAAAATTCAGCTAGAATATGAATGGCTAGAAGGGAAGGTTCTCCATGTGGACGTCGAA
GATGCCCGTCATCACGACGCCGCCTACGGAGCGTCCCTTCTATCGACCATCCAAGAGGGAGACCTTTGTTTGAAAGACTTAGGCTATTTTTCGCTTGAGG
GATTGCAAGCCATTCATGACGCCGGGGCCTTTTATATTTCACGGCTCAAACACAATGTAGGGATTTACCAAAAAGAAGGGGACCGATTTCGAAAATGGGA
ACCAGAGGATTTCCTTGCGGTGCTGCAACCGGGAGAAACCATGGAACTGGAACATGCGTATGTCAGTGGAAAGAAAGTTCATCAACCGCGGCTCATTGTG
TATCGGCTGACAGAAGAACAGGAGCGACAGAAGGAAGGACAATGGAAACAGAAGGCGAAACAAAAAGGGGCCGCGTACGTGACGCGACGCCCCCACCCAA
TATATGTATATATCACCAATATTCCAGCGATTTATACCTCTTTGCACGAAATTCATACGCTGTATTCTCTTCGCTGGCAGATTGAGGTGGTATTTAAGAC
ATGGAAATCCCTGTTTCACATTCATCGATTCAAACCGATGAAAGGGGCTCGCTTTCAGTGCCACCTGTATGGAACATTGATTGCCTTATTGATCAGCTCC
ACTGTCATGTTTAAAATGCGGGAATGGCTCTATCGAAAACAGAAAAAGGAATTGAGCGAATATAAGGCGATGAGTATGATCAAGGAGTTCGGTATGGATT
TATTTCAAGCCTTATGGTGTTCGGAGGCGTTAGCTGTCCAACTTCTTTTAAAACTGTGTGACATCATCGCTCAACACGGAAAAAAATCAAGGCGTTATAC
AAAAAAGAGCGCTTTAGACATTATCGAGAGTTTGGTCATCATGCCGGTGTCGTAAGAAATTCCCGCCTCATACCATGAAAAAAACCTTCTATCGGGAAGG
TGTATTTGGCATGCCTATCTTTATATATTCTCACGGTCACAGCCTCCAAAGATGAAGAGTGAAGCCTTGGAGCTACTCGCTCCAAGGCTTCACTTGATGG
CTATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1359 bp | 453 aa | 94 | 1452 | + | No |
Chemistry : DDE
ORF sequence :
MKTSLSLQEEWHCFLDGLRSVLSCEELEHMARDHQFIQRKGKLRAHDFVALCTFLQEGGGQKSLVQLCSALALKQNTSLSAEGLNQRFHEKAVSFLKAVF
EKLLIHQTQEARRLCPRHSLFLRIRILDSTSFQLPPEIQGIYEGCTGPGVKIQLEYEWLEGKVLHVDVEDARHHDAAYGASLLSTIQEGDLCLKDLGYFS
LEGLQAIHDAGAFYISRLKHNVGIYQKEGDRFRKWEPEDFLAVLQPGETMELEHAYVSGKKVHQPRLIVYRLTEEQERQKEGQWKQKAKQKGAAYVTRRP
HPIYVYITNIPAIYTSLHEIHTLYSLRWQIEVVFKTWKSLFHIHRFKPMKGARFQCHLYGTLIALLISSTVMFKMREWLYRKQKKELSEYKAMSMIKEFG
MDLFQALWCSEALAVQLLLKLCDIIAQHGKKSRRYTKKSALDIIESLVIMPVS
EKLLIHQTQEARRLCPRHSLFLRIRILDSTSFQLPPEIQGIYEGCTGPGVKIQLEYEWLEGKVLHVDVEDARHHDAAYGASLLSTIQEGDLCLKDLGYFS
LEGLQAIHDAGAFYISRLKHNVGIYQKEGDRFRKWEPEDFLAVLQPGETMELEHAYVSGKKVHQPRLIVYRLTEEQERQKEGQWKQKAKQKGAAYVTRRP
HPIYVYITNIPAIYTSLHEIHTLYSLRWQIEVVFKTWKSLFHIHRFKPMKGARFQCHLYGTLIALLISSTVMFKMREWLYRKQKKELSEYKAMSMIKEFG
MDLFQALWCSEALAVQLLLKLCDIIAQHGKKSRRYTKKSALDIIESLVIMPVS
Blast result :
Comments
ISGka3 is 59% aa similar to IS231Y.
ISGka3 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-66-D(N3)-135-E(C1). The copy number in Geobacillus kaustophilus HTA426 is 1.
ISGka3 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-66-D(N3)-135-E(C1). The copy number in Geobacillus kaustophilus HTA426 is 1.
References
1] Takami,H., Takaki,Y., Chee,G.J., Nishi,S., Shimamura,S., Suzuki,H., Matsui,S. and Uchiyama,I.(2004) Nucleic Acids Res. 32(21), 6292-6303
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18