ISfinder Mirror Site

ISCb2

Family IS4
Group IS231

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Clostridium beijerincki	Clostridium beijerincki NCIM B8052

DNA section

IS Length : 1774 bp

Ends

IR Length : 15

IRL : CATACGCGTAAAGCTAATGAGTATTTTAACAGTTAAATCATATATTAACA
IRR : CATACGCGTAAAGCTTAACCAGTAATCACCAAATTGTAGGGGGAAGAGAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTAACTTAAA	GTTGGGTTATC	TATAGAATAA	11

DNA sequence

CATACGCGTAAAGCTAATGAGTATTTTAACAGTTAAATCATATATTAACAGTTATAAAAATAATAAAAAAAGTCATTCCTAACAATAAACACAAAAAAGA
AAGGAATGACTTTTAATGAAAATTAATTTAAATTCATTAAGTAGTATGGATAAAATTAAGAAAATTATTAATCTTTTTTCAAAAAGATTAATAACAAAAA
CCGCCGTGACTACTGGATTTACACAAAGAAATAGTAAATTGGATGGATTTACATTTTTTAAAGCATTTACTTTTGGAGTTTATAGTCTTGAAAATCCATC
ATTAAGAAATATAGCAAATTTCTGTGAAGATATAAACCCTAATTTGAAAGTGTCTAGGCAAGCTATAGAAAATAAGCTCAAGGCAGGTTCAAACTTTTTA
AAAACTATTCTTACAAATATTATTGAGGATAAAATAATTAAGAGCATTAAACACAATCACATTGAAATATTTAAAGCCTTTAATGATATAAAAATCTGCG
ATAGTAGCTTAATCAAGCTAAATGACTCGTTAAGAGATTCTTATAAAGGTTTTTCAGAAGATAAGTCGGCATCTGAAATGAAAATACAAACAGTATATAG
TTTCAAAAGTAAGCAGATTGAAACATTTGAATTTGAGGATGGCACAACAAATGATAATTCATATATGAAAACTTTGGCGGACAAAATTAACACTAATGAA
ATTCTATTAGTTGATTTAGGATATTTCGATAAAAAGTGTTTTAAAATGCTTGAAAAGAAATCTGCTTTTTTTCTTAGTAAAATTAAATATAATACTGCAC
TTTATAAAGAAAATTATAAAAAAGGGAATTTTGAAAAAGTTGAAATGATTGATTTCTTAAAGAAATCGAGCGGTGTTATAGATACTTATTTATATGTTGG
AATGAAACAAAATAATAGAGAAGAATTTAGAGTAATAGGAAAAAGATTACCCGAAGAAATTGTGAATTTGCGAATCCGAAGGGCTAGAGAAAAAGCAAAA
GCTCAAGGAAGAGCCCCAAAGAAAATTGACAAGGAACTAATGAGCTGGGTTATTATGATAACAAACATTGAAAAAGAACAAGCCGATGTGGACATGCTCC
TTGATATTTATAGGTTAAGATGGCAAATAGAACTCTTATTTAAATGCTGGAAAAGCTACGGAAAGATTGACCATGTCAAATCAGCAGGAATTGATTACTT
AAATTGTTTGCTTTATGGTAGATTGATAATAACTTTACTAATAAATACAGTTTATTCTGAATTGTATTTTAAGTATAAAACAGAAGAAAATAGAGAAATA
AGTATGCTCATGATATATTCAACTATAGGAAGTAAACTTAAAGAAATATGCCTTAATTTCATTCCTAAAAAGGAAAACTTGGAAATAATATATGAGATAC
TAGTAAGTTTAGGAAGGAGCTGTAAGCGAGAAAAAAGAAAACGCCAAACTACAGAGGGAAAATTAATGAGCTACTCTCTTCCCCCTACAATTTGGTGATT
ACTGGTTAAGCTTTACGCGTATG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1380 bp	460 aa	116	1495	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKINLNSLSSMDKIKKIINLFSKRLITKTAVTTGFTQRNSKLDGFTFFKAFTFGVYSLENPSLRNIANFCEDINPNLKVSRQAIENKLKAGSNFLKTILT
NIIEDKIIKSIKHNHIEIFKAFNDIKICDSSLIKLNDSLRDSYKGFSEDKSASEMKIQTVYSFKSKQIETFEFEDGTTNDNSYMKTLADKINTNEILLVD
LGYFDKKCFKMLEKKSAFFLSKIKYNTALYKENYKKGNFEKVEMIDFLKKSSGVIDTYLYVGMKQNNREEFRVIGKRLPEEIVNLRIRRAREKAKAQGRA
PKKIDKELMSWVIMITNIEKEQADVDMLLDIYRLRWQIELLFKCWKSYGKIDHVKSAGIDYLNCLLYGRLIITLLINTVYSELYFKYKTEENREISMLMI
YSTIGSKLKEICLNFIPKKENLEIIYEILVSLGRSCKREKRKRQTTEGKLMSYSLPPTIW

Blast result :

Comments

ISCb2 is 45% aa similar to ISBce2. ISCb2 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-138-E(C1). The sequencing of the Clostridium beijerincki NCIM B8052 chromosome is currently in progress. Accession number: NZ_AALO00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2005)Unpublished.