ISBce11

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Bacillus cereus
Bacillus cereus G9241
DNA section
IS Length : 2928 bp

Ends


IR Length : 17

IRL : CATGCCCATCAAGCTAAGATTTTAAGACACCCCCATAACGAGAATTTTAC
IRR : CATGCCCATCAAGCTAATCAAGTAATTTACTAGATATGAAACGATCCTTG

Insertion site


Left flankDirect repeatRight flankDR Length
CGCGTCTTGTTGGCGGNACCCCACATCCATC11

DNA sequence

CATGCCCATCAAGCTAAGATTTTAAGACACCCCCATAACGAGAATTTTACTTTTTTACAGTTATTTATGAAAATTCCGCTTGTTTTTTTCATTTTTTGGG
TGCTAGACCTAAAACTTCAGCAATATGCATAGACTGAAACATCTAGTTTTCACGATATAATTCTCAGCTAAATTCGAAAAAAAGAGCTTGAATTCCCAAG
CTCTTTTAAATATTACTTAATAACTTTATAACCACCATTTTTCCTGTGGAATTAAAACTGTTTCAATATCAAAGTTATTCTCAAACCATTCCTTCATCAA
TGTTTTATGGACCAGATATTCTGATGCAGAATGGGAAACGCCGATTAAAGACATCGAAGTATTAGAAACATAATCCATCATTTGTTTGTATCTTTGTTTA
CCATATTCGTTATCAATATGGCAATGGATTTCGCCAGTGATATAGGCTTGAACTCCTCTTTCTTCAGCTTCCTCCATCCAACTTACTTTGTCGCCACAGC
CTGCAACGATAGCTATTTTCTTTACGTTATTTATGTTCTTACCTTCAAAATCAACATATGGAATATCGAATATTTCTTTTAAGTGAGAAGTTAGTTCACT
TGTACTCTTTTCTTCGATGTTACAAATTAGAACTAGGTCTTCATTATTTATAGAACTTGTTAAAAACCCATCAATAATTTCTGCATTTAATTGTTTAGCA
ATAGCCACACTTGTACCTAAATTGGCATGATAATCCATTGGTACATGGCATGTATAGAATGATAATTCCTTTTCTTTTATTTGTTTAATGTATTTTTCTT
TTATAGGAACAAAGCCTTTGCCCCATACTCCTTTGGGATCTCCACATTCCATCAATAAGGGATGGTGCATAAAAAGTAAGTCGCCGCTATTACTTTCTTC
TATAAAGCGTTCCAACACTTCATCTGTAGGGAAAACGGCAAGAAAAATTTTCTTTACATTTTCATCTCCTTTTAACATTAGACCATTAAATAATTCCACA
AAGTCTTTTTCAAAAACTGATTTCCAGTCAAACTTTAAAGGTTCATAGACCATTGGTATGAATTGACTGAAAGCAGAATCCTTCCCAAATGATTTTATAT
CAAGTTCAATGTCCAGTTTAGACACTATTTCACTTAGTTTAACCATAAAATTATTCCCCCAAAAAATTTTATAATTCAATACAAACGGTTAGAAAAATAA
CAATTATTATATTCTTTTAAATATTTTAACATATAAATCCATTTTTCTTCATGAAAAAATCACTCCTGTTCTTTAAAGTGGTCCCTCCACATTCCCTTCA
AGCAAAGTTAAAATCATACCTTCAAAATGAAAAAACACTATGCTTATTGTAAACAGTTTATAAGAAAGGATGGCGTTTTGATGAAACCTATCATTTTAAA
TAGATTGAATCTTTTCGCTCAAGAGCTACAATACTTTTTATCTCCAGTGGTCCTACAAGATATTGCCAAACAAGTTGGCTTTGTACAGCGATCTAGTAAG
TATCAAGTAAAAGAACTCATTGCACTTTGTGTATGGCTTAGTCAAGAAATTGCTAGCACATCACTTACACAATTGTGTAGTCAGTTAGAAGTTTCCACTG
GGGTACTTATGAGCTCAGAAGGACTGAATCAACGCTTTAACCCAGCAGCTGTCGCATTTCTTCGAGAAGTCTTTACTTCTCTTCTCACACAAAAACTCTG
TTCAAATCACTCACTTTCTGCACACATGATCCCTACTTTCAATCGTATCCGTATTTTGGATGCAACAGTGTTTCAACTACCTGATCATTTCGCCACTGAC
TATCAAGGTTCAGGTGGAAGTAGTAATACGGCAGGCGTAAAAATCCAATTGGAATATGATTTACTTAGTGGTCAATTTCTTAACGTGCAGCTTGGACCAG
GAAAGAATAATGATAAAACATACGGTACCATCTGCCTTGAAACCGTAGAGACCAACGATTTGTGTTTACGTGATCTTGGTTACTTCGATTTAGGAGACTT
ACAAACTATTCATGATAAAGGGGCTTACTACATCTCGCGGTTGAAGCTAAATACGCGGATTTATATCAAGAACCCTGAGCCAGAATACTTCAACAACGGC
ACGTTAAAAAAGCAAGCAGAATACATCCAGCTTGATATGACACAAATGATGTCAGATCTAATCCCTGGTGAAACGATAGAAATTCCCGAGGCATACATTG
GCCAAAATCAAAAACTTCCAGCACGTGTCATTATCCATCGTTTAACCGATGATTATACGCAAACACGCCTAAAAAACCAAGCGATACGTGAAAAGAAAAA
AGGCATTGTCATGAAGGATAAAAGTAAACGTTTAATGGGCATGAATGTATATATTACGAACACGTCTTTAGAAGAAGTACCAACTAACTACGTGCACTCA
CTGTATTCACTTCGTTGGCAGATTGAAATTTTGTTTAAAACGTGGAAGTCATTTTTTGAAATTGATGAATGTAAAAACATTAAAAGAGAGCGCCTAGAGT
GCCATTTATATGGACAACTAATCGGTATTCTCCTCTGTTCTTCTACGATGTTTCAAATGCGACAGTTTTTGCTTGAAAAAACAAATCAGGAGCTCAGTGA
ATACAAAACGATTTATATGATTAAAGATTACTTTCCGTTACTGTTTCAAGCGATAGCCATTGGCACAGAACAACTTTTGAAAATTTTGCATCGTCTTTAT
CTATTGCTCAAAAAGAACGGTCGTAAATGTCATCGGTATAAGAAAATGACCGTCTTTGATATTCTAAGGATTGTGTATAAAACGACGGTGAAGCATAGAC
AAACTGCCTAGCGAAAAAATCAGATTTTAGCAGGCTTCTTTAGTATGCCTACTTTTCTATCAATTGTTAGTTTTGAACCAAGGATCGTTTCATATCTAGT
AAATTACTTGATTAGCTTGATGGGCATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1428 bp476 aa13812808+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPIILNRLNLFAQELQYFLSPVVLQDIAKQVGFVQRSSKYQVKELIALCVWLSQEIASTSLTQLCSQLEVSTGVLMSSEGLNQRFNPAAVAFLREVFTS
LLTQKLCSNHSLSAHMIPTFNRIRILDATVFQLPDHFATDYQGSGGSSNTAGVKIQLEYDLLSGQFLNVQLGPGKNNDKTYGTICLETVETNDLCLRDLG
YFDLGDLQTIHDKGAYYISRLKLNTRIYIKNPEPEYFNNGTLKKQAEYIQLDMTQMMSDLIPGETIEIPEAYIGQNQKLPARVIIHRLTDDYTQTRLKNQ
AIREKKKGIVMKDKSKRLMGMNVYITNTSLEEVPTNYVHSLYSLRWQIEILFKTWKSFFEIDECKNIKRERLECHLYGQLIGILLCSSTMFQMRQFLLEK
TNQELSEYKTIYMIKDYFPLLFQAIAIGTEQLLKILHRLYLLLKKNGRKCHRYKKMTVFDILRIVYKTTVKHRQTA

 

Blast result :
Comments
ISBce11 is 76% aa similar to IS231Y. ISBce11 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-150-E(C1). The sequencing of the Bacillus cereus G9241 chromosome is currently in progress. Accession number : NZ_AAEK00000000.
The transposase upstream sequence harbors a putative passenger gene that shows similarity with NIF3. This protein family contains several NIF3 (NGG1p interacting factor 3) protein homologues. NIF3 interacts with the yeast transcriptional coactivator NGG1p which is part of the ADA complex, the exact function of this interaction is unknown.
References
1] ,De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Hoffmaster,A.R., Ravel,J., Rasko,D.A., Chapman,G.D., Chute,M.D., Marston,C.K., De,B.K., Sacchi,C.T., Fitzgerald,C., Mayer,L.W., Maiden,M.C.J., Priest,F.G., Barker,M., Jiang,L., Cer,R.Z., Rilstone,J., Peterson,S.N., Weyant,R.S., Galloway,D.R., Read,T.D., Popovic,T. and Fraser,C.M.(2004)In press