ISBce10
- Family IS4
- Group IS231
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007105 | ND | Bacillus cereus | Bacillus cereus E33L |
DNA section
IS Length : 2649 bp
Ends
IR Length : 19/21
IRL : CATGCCCATCAAGCTAAGGTTTTGGTGTCCCCCCACAACGATAAAAATAA
IRR : CATGCCCATCAACCTAAGATTAAGTAAAAGTCCGTTCAACTGGATTTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTATAACCA | TGGCGGTACC | 0 |
DNA sequence
CATGCCCATCAAGCTAAGGTTTTGGTGTCCCCCCACAACGATAAAAATAAGCTTGGTTGCCATAGAAAACATCACGTTTTCATTCTGAAAGCATTATAGA
AGTTTAACCTAATGATTATATAGCAGGTACTGATTATTAAAGAAAAAATGCAATCGGATTAATCGCTACATCAATAAAGATAATTCAAAAAACACCCTTC
ATTAATAACTAATGAAGGGTGTTTTGAATTAATCATTATAGTTCGTCTTCTTCAACTTTTTTACTATTATACTTACTATATCCATATATACATAAACCGA
AAAATACTCCACCCATACTATTTATAGTAAGATCTCTCATGGTAATTGCTTCTCCAGAAGGTAAATATGTTAATAAAGTAAATACAACAAAGAAAATAAA
TCCTGAGAATATCGAGTTACGAACTAACATTTTATTATTTGATGTATAACGTATATATATTTTTTTGATTCGCATCATCATAACGATATAAAATGATGTT
GTTAATAAAATAATTAAGGCTAGAATGCCAAGTGATGTAATTTCTTTGTTTAAAATCAAGTCATATAACATAGCTCCAAAACAGATGAAAAGAGCTAACA
TCCCAGCGTTTGCCATTATTTCGAAGGCTGTATTTTGTTCTCGTTCATATGAGAAGAAAACAGTCTTTGATATATTGGGTGTCATATACAATTGTTTTGT
TACTCACAATCATGTAGCTTAATAAAAAAAATGAAACCCGTTAGGGTTTATTTGGTATGCAGATTTTTAGATAGCTGATATCAACTCTTTAGAAAATAGA
AAAAATCCAGTTGAACGGACTTTTACTTAATCTTAGGTTGATGGGCATG
AGTTTAACCTAATGATTATATAGCAGGTACTGATTATTAAAGAAAAAATGCAATCGGATTAATCGCTACATCAATAAAGATAATTCAAAAAACACCCTTC
ATTAATAACTAATGAAGGGTGTTTTGAATTAATCATTATAGTTCGTCTTCTTCAACTTTTTTACTATTATACTTACTATATCCATATATACATAAACCGA
AAAATACTCCACCCATACTATTTATAGTAAGATCTCTCATGGTAATTGCTTCTCCAGAAGGTAAATATGTTAATAAAGTAAATACAACAAAGAAAATAAA
TCCTGAGAATATCGAGTTACGAACTAACATTTTATTATTTGATGTATAACGTATATATATTTTTTTGATTCGCATCATCATAACGATATAAAATGATGTT
GTTAATAAAATAATTAAGGCTAGAATGCCAAGTGATGTAATTTCTTTGTTTAAAATCAAGTCATATAACATAGCTCCAAAACAGATGAAAAGAGCTAACA
TCCCAGCGTTTGCCATTATTTCGAAGGCTGTATTTTGTTCTCGTTCATATGAGAAGAAAACAGTCTTTGATATATTGGGTGTCATATACAATTGTTTTGT
TACTCACAATCATGTAGCTTAATAAAAAAAATGAAACCCGTTAGGGTTTATTTGGTATGCAGATTTTTAGATAGCTGATATCAACTCTTTAGAAAATAGA
AAAAATCCAGTTGAACGGACTTTTACTTAATCTTAGGTTGATGGGCATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1428 bp | 476 aa | 1092 | 2519 | + | No |
Chemistry : DDE
ORF sequence :
MDLSISDELELFSKELQRYISPHVLEQLAREIGFVQRKSKYRAQDLVALCVWLSQNIAHTSLTQLCSRLETNTGISMSPEGLNQRFNSQAVHFLQQILTY
LLHQQLCTSSKISTLYTNYFRSIRVLDSTHFQIPDKFASTYQGSGGSGHSAGVKIQLEYDLLSGQFLHVHVGSGKHNDKTYGSTCLTSLQRHDVCIRDLG
YFDLRDLHTIDECGAYYISRLKLNTRIYQKNREPEYFQNGTIKKHSEYVQLDMEQFIDQLQSGETYEIPEIYIGMYQKLPARLILYKLTETQMKRRLKDL
ASKEHKKQITYKERSKRLSAINFYITNIPSEYLPREQVYDFYSLRWQIELIFKTWKSFFRIHHCNSVKLERLECHLYGQLISILLCSSTMFQMRQLLLTK
KKQELSEYKAVYIIKDYFLLLYQALQKDTQEISKILLRLFNFLQKNGRKSHRYEKKTVFDILGVIYNCFVTHNHVA
LLHQQLCTSSKISTLYTNYFRSIRVLDSTHFQIPDKFASTYQGSGGSGHSAGVKIQLEYDLLSGQFLHVHVGSGKHNDKTYGSTCLTSLQRHDVCIRDLG
YFDLRDLHTIDECGAYYISRLKLNTRIYQKNREPEYFQNGTIKKHSEYVQLDMEQFIDQLQSGETYEIPEIYIGMYQKLPARLILYKLTETQMKRRLKDL
ASKEHKKQITYKERSKRLSAINFYITNIPSEYLPREQVYDFYSLRWQIELIFKTWKSFFRIHHCNSVKLERLECHLYGQLISILLCSSTMFQMRQLLLTK
KKQELSEYKAVYIIKDYFLLLYQALQKDTQEISKILLRLFNFLQKNGRKSHRYEKKTVFDILGVIYNCFVTHNHVA
Blast result :
Comments
ISBce10 is 81% a similar to IS231Y.
ISBce10 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-150-E(C1). ISBce10 is located on plasmid pE33L54. The copy number in Bacillus cereus E33L is 1.
ISBce10 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-150-E(C1). ISBce10 is located on plasmid pE33L54. The copy number in Bacillus cereus E33L is 1.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18