ISAs5

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY129557 ND Aeromonas salmonicida
Aeromonas salmonicida
DNA section
IS Length : 1233 bp

Ends


IR Length : 27/44

IRL : TGAATCGCCCCCAGTTCCCTAGACACCTCACAGCCTTAAAATGAGGCAAC
IRR : TGAATCGCCCCTGGTTTTCTAGACACTCTGTTGCTGACAAATAAACTGCC

Insertion site


Left flankDirect repeatRight flankDR Length
CCATGATGCGACCATTCGCCATA3

DNA sequence

TGAATCGCCCCCAGTTCCCTAGACACCTCACAGCCTTAAAATGAGGCAACTATTGGAGGTGTTATGACCCGTCAGCGTTATCCAGAAACATTCAAAATCG
AAGCGGTTAAACAAATTACCGAGCGTGGCCGGCCGGTGGCTGACGTAGCACGAGCCCTCGGGGTATCCAGCCACAGCCTGTATGCATGGCTCAAGCAGTA
CGGTAAGCCCGCTGATCAGCAGCACGAAGAGTCAGCCTTACAAGCCGAGATCCGCCGTCTGAAAGCCGAACTGCGCCAAGTCACTGAGGAGCTGCAACAT
TTTAAAGGAGGCCGCCGCGTACTTTGCCAGCGAGTCAAGGAAAGGTACGCGTTCATAACCTCCCGACTCGACCGGTACAGTGTCGTTACCCTGTGCCAGA
CCCTGGGGGTTCACCGAAGTGGCTTCTACGCGTGGCTGAAACAGCCCGCATCGTTACGCCAGCAAGCGGATGAACGGCTGACCGGATTGGTCAAGCAATC
CTGGCTGGAAAGTGGTGCTGTCTACGGTTATCGCAAGATCCATCGCGATCTATGTGACTTGGGGGAAGCCTGCGGCAAGCACCGTGTGGCCAAATTAATG
AGGCGGGAGGGGCTGCGTTCGCAAACTGGCTATCAACGCCGTAAGGGGCATTATGGAGGCAAGAGCCCACAGGCTGCCCCAAACACGCTGGCACGTCAAT
TTAAGGTGCCAGCCCCCAACATCTCCTGGGTGACTGACATTACCTACATCCGAACTCAAGAAGGGTGGTTGTACCTGGCGGTGGTGCTCGATCTGTTTTC
TCGCCAGATCATCGGCTGGGCGATGAAGCCACGCATGACGGCAGGCTTGGCGGTTGATGCGTTATTGATGGCGGTTTGGCGACGTAAGCCAACGCAGCCG
GTACTGGTGCATTCGGATCAGGGCAGCCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCCCACAACCTGAGTTGCAGTATGAGCCGACGTGGTA
ACTGCCACGACAATGCTGTCGCCGAGAGCTTCTTTCAGTTGCTCAAACGGGAGCGGATCAAGCGCAGGATTTACTCGACAAGAGACGAAGCCCGCAGCGA
CGTATTTGATTATATCGAAATGTTTTACAACCCAATCAGGCGCCATGGTTCCAATGATGGGCTGTCGCCGGTAGAGTTTGAAAGGCAGTTTATTTGTCAG
CAACAGAGTGTCTAGAAAACCAGGGGCGATTCA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1152 bp383 aa641215+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRQRYPETFKIEAVKQITERGRPVADVARALGVSSHSLYAWLKQYGKPADQQHEESALQAEIRRLKAELRQVTEELQHFKGGRRVLCQRVKERYAFITS
RLDRYSVVTLCQTLGVHRSGFYAWLKQPASLRQQADERLTGLVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGK
SPQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLK
AHNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQSV

 

Blast result :
Comments
ISAs5 is 82% aa similar to ISPen2. There is no programmed -1 frameshift.
ISAs7, an isoform of ISAs5, carry a frameshift.
References
1] Merino,S., Gavin,R., Vilches,S., Shaw,J.G. and Tomas,J.M. (2003) Appl. Environ. Microbiol. 69 (1), 663-667