ISEfa8
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAAK03000001 | ND | Enterococcus faecium | Enterococcus faecium DO |
DNA section
IS Length : 1366 bp
Ends
IR Length : 30/41
IRL : TGAATCGGCTACACTAGACTAGACAGAAAAAATAAGGTGTGTAGAATAAT
IRR : TGGAATGGCAACACTTTTTTTGACAAAAATTATAAGGTGTTGTGAATGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCCTTATCC | TCTGGCATTA | 0 |
DNA sequence
TGAATCGGCTACACTAGACTAGACAGAAAAAATAAGGTGTGTAGAATAATAAAAAACACACTGGAGGATTTTTCATGCCAAGACGTCAACGAAGAACCTA
TTCAAAAGAATTCAAACAACAAATCGTCGATCTCTATCTCGCTGGTAAGCCTTGCGCAGGAATTATTCGAGAGTATGAGCTTACGCCTTCTTCTTTCGAT
AAATGGATGAAGCAAGCACAATCAACGGGCTCATTCAAAGAAAGAGACAACTTAACACCAGAACAAGCAGAATTGATCGCACTAAGAAAGAAAAATAAGC
AACTCGAAATGGAGAATGATATTTTAAAGCAAGCGGCGCTGATATTCGGACGAAAAGACAAGTAATTGATGCCAACAAGCATAAATATTCCATATCAGCG
ATGTGCAAAATTCTAAATATTTCTCGTCAGACCTACTATTATCAAGCGAAACCGATCGAAAATGAGTCCGACTTAGAAGAGATCGTTCAGGAAGAGTTTA
TTCGAAACCGAAAGGCTTACGGTACCCGAAAATTGAAGAAGTGTTTAGCAAAGCGTGGGCTTCAACTCAGTCGGCGCCGGATCGGTCGAATCATGAAACG
CCGCGGATTGACATCTACCTATACGATCGCTCATTTTAAAGGGCAACGAACAGCTTGTAATGAAGCGAAAACAGCGAATGTATTAGATCGGACCTTTACA
CAAGAACAGCCATTGGAAGCCATCGTTACGGATCTTACTTATGTTCGCGTGAGGAAAAAGTGGCATTATATCTGCTTAATACTTGATTTGTTTAATCGAG
AAATTATTGGTTATTCCTGTGGTGAGAAGAAAGATGCCTCATTGGTAAAAGAAGCCTTTGGACGGATACCGTATTCTTTAACAGACGTCAAGCTTTTTCA
TACAGACCGTGGAAAGGAATTTGATAACCAAACCATTCATGAGATTCTGAATGGTTTTGGAATTACTCGTTCATTGAGTAGGAAGGGTTGTCCGTATGAT
AATGCCGTTGTGGAATCAACCTATAAATCTGTCAAAGTAGAATTCGTGCATCAATACCAATTTGAGACACTGGCACAGCTACGTCTAGAATTGTTTGATT
ATGTGCATTGGTGGAACTATCTACGCTTACATGGCACGTTGGCGTATGAAACACCGATCCAAATTCGACAACAGAGATTGGCGAAGCGAATCCTTGATAA
TGAGCGCGGATCTGATACCTCTGGAGAGGCAGCGTAATTGAATGATTGTGCTTCTGCCGGAGAAAATCAGATCCGAGGATGCTCATTGTCAAGGGCAATC
GTAGCCATAACACCGCAGCATTCACAACACCTTATAATTTTTGTCAAAAAAAGTGTTGCCATTCCA
TTCAAAAGAATTCAAACAACAAATCGTCGATCTCTATCTCGCTGGTAAGCCTTGCGCAGGAATTATTCGAGAGTATGAGCTTACGCCTTCTTCTTTCGAT
AAATGGATGAAGCAAGCACAATCAACGGGCTCATTCAAAGAAAGAGACAACTTAACACCAGAACAAGCAGAATTGATCGCACTAAGAAAGAAAAATAAGC
AACTCGAAATGGAGAATGATATTTTAAAGCAAGCGGCGCTGATATTCGGACGAAAAGACAAGTAATTGATGCCAACAAGCATAAATATTCCATATCAGCG
ATGTGCAAAATTCTAAATATTTCTCGTCAGACCTACTATTATCAAGCGAAACCGATCGAAAATGAGTCCGACTTAGAAGAGATCGTTCAGGAAGAGTTTA
TTCGAAACCGAAAGGCTTACGGTACCCGAAAATTGAAGAAGTGTTTAGCAAAGCGTGGGCTTCAACTCAGTCGGCGCCGGATCGGTCGAATCATGAAACG
CCGCGGATTGACATCTACCTATACGATCGCTCATTTTAAAGGGCAACGAACAGCTTGTAATGAAGCGAAAACAGCGAATGTATTAGATCGGACCTTTACA
CAAGAACAGCCATTGGAAGCCATCGTTACGGATCTTACTTATGTTCGCGTGAGGAAAAAGTGGCATTATATCTGCTTAATACTTGATTTGTTTAATCGAG
AAATTATTGGTTATTCCTGTGGTGAGAAGAAAGATGCCTCATTGGTAAAAGAAGCCTTTGGACGGATACCGTATTCTTTAACAGACGTCAAGCTTTTTCA
TACAGACCGTGGAAAGGAATTTGATAACCAAACCATTCATGAGATTCTGAATGGTTTTGGAATTACTCGTTCATTGAGTAGGAAGGGTTGTCCGTATGAT
AATGCCGTTGTGGAATCAACCTATAAATCTGTCAAAGTAGAATTCGTGCATCAATACCAATTTGAGACACTGGCACAGCTACGTCTAGAATTGTTTGATT
ATGTGCATTGGTGGAACTATCTACGCTTACATGGCACGTTGGCGTATGAAACACCGATCCAAATTCGACAACAGAGATTGGCGAAGCGAATCCTTGATAA
TGAGCGCGGATCTGATACCTCTGGAGAGGCAGCGTAATTGAATGATTGTGCTTCTGCCGGAGAAAATCAGATCCGAGGATGCTCATTGTCAAGGGCAATC
GTAGCCATAACACCGCAGCATTCACAACACCTTATAATTTTTGTCAAAAAAAGTGTTGCCATTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 75 | 365 | + | No |
Description : First part of the transposase
ORF sequence :
MPRRQRRTYSKEFKQQIVDLYLAGKPCAGIIREYELTPSSFDKWMKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGRKDK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 320 | 1237 | + | No |
Description : Second part of the transposase
ORF sequence :
YFKASGADIRTKRQVIDANKHKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTST
YTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKE
FDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDT
SGEAA
YTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKE
FDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDT
SGEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 75 | 1237 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRRQRRTYSKEFKQQIVDLYLAGKPCAGIIREYELTPSSFDKWMKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGRKRQVIDA
NKHKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKT
ANVLDRTFTQEQPLEAIVTDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRS
LSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
NKHKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKT
ANVLDRTFTQEQPLEAIVTDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRS
LSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
Blast result :
Comments
Genome in progress.
ISEfa8 is 91%(ORFA) and 84%(ORFB) aa similar to IS1485. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.
ISEfa8 is 91%(ORFA) and 84%(ORFB) aa similar to IS1485. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2002) CONSRTM US DOE Joint Genome Institute (JGI-PGF)