ISAisp2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008782 | ND | Acidovorax sp. | Acidovorax sp. |
DNA section
IS Length : 1241 bp
Ends
IR Length : 33/40
IRL : TGGATGAATCGCCCCGGTTGAACTAGACACCTTCGAGCCTCAAACCTGAG
IRR : TGGGGTGAATCGCCCCGGCATTCCTAGACACTTTTGAGCCGTTGGGAATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGCTGGGCG | TGCTGGCGGG | 0 |
DNA sequence
TGGATGAATCGCCCCGGTTGAACTAGACACCTTCGAGCCTCAAACCTGAGGCCCAATAGGAGGTGCCATGACAAAGCAGCCATATCCCGAAGAATTCAAG
ATCGAAGCGGTCAAGCAGATCACGGAGCGAGGCCACCGGGTGGCCGATGTATCCGCCCGGATCGGCGTGAGCCAGCACAGCCTGTACAAGTGGATCAAGG
CCTATGCAGTGCCCGCGCCAGAACGACAGATGCAGACGTCCCAGACCGAGGAGCTGCGGCGCCTGAAGGCTGAACTCAAGCGCGTGACCGAGGAGCGCGA
CATCCTAAAAAAAGCCGCAGCGTACTTTGCCAGGCAGTCCGGGTGAGGTACGCATTCATCAAGCAGCACGAGCAGGAACACAGCGTGCGGCGCATGTGCA
AGGTGATGCAAGTCCACCCCAGTGGCTACTACGCATGGAAGGCAGCGCCGCAGAGTCCACGGGCTAAGGACGACCAGAGGTTGCTGGGCTTGCTCAAGCA
GGCATGGCTGGAGAGCGGCGGTGTCTATGGCTACCGCAAGCTCACGCTGGACATGCGCGACCTGGGCGAGCGCTGCGGCAAGCATCGCGTTGCGCGGTTG
CTCAAGCTCGAAGGCCTGCGCTCGCAGACGGGCTACGGCCGCAGGCCTGGGCGGCGAGGCGGCAAGCCTGCAGTGGTCGCACCAAACCACCTGCAGCGCC
AGTTCAAGGTGGATGAGCCCAATCAGTCCTGGGTCACCGACATCACCTACATCCGAACCCACGAAGGCTGGCTGTACCTGGCGGTTGTGGTCGATCTGTT
CTCGCGCCAAGTAGTGGGCTGGTCCATGGGCAGCCGCATTGACACGAGCCTGGTCCTCGATGCACTGCTGATGGCGCTATGGCGCCGCCAGCCGCATGCG
CCTGTCATGGTTCATTCCGATCAGGGCTGTCAGTTCACCGGCCACGAGTGGCAGACCTTCCTGCGCGAGCACAACCTGGTCAGTAGCATGAGCCGGCGTG
GCAACTGCCACGACAACGCCGTGGCCGAGAGCTTCTTCCAACTGCTCAAGCGCGAACGCATCCGCAGGCGGGTCTACGAAACCCGTGACGATGCGCGAGC
GGACGTCTTCAACTACATTGAAATGTTCTACAACTCACAGCGCCGTCATTCATCGGCGGCGGGACTATCACCTGTAGAGTTCGAACAGCGACATTCCCAA
CGGCTCAAAAGTGTCTAGGAATGCCGGGGCGATTCACCCCA
ATCGAAGCGGTCAAGCAGATCACGGAGCGAGGCCACCGGGTGGCCGATGTATCCGCCCGGATCGGCGTGAGCCAGCACAGCCTGTACAAGTGGATCAAGG
CCTATGCAGTGCCCGCGCCAGAACGACAGATGCAGACGTCCCAGACCGAGGAGCTGCGGCGCCTGAAGGCTGAACTCAAGCGCGTGACCGAGGAGCGCGA
CATCCTAAAAAAAGCCGCAGCGTACTTTGCCAGGCAGTCCGGGTGAGGTACGCATTCATCAAGCAGCACGAGCAGGAACACAGCGTGCGGCGCATGTGCA
AGGTGATGCAAGTCCACCCCAGTGGCTACTACGCATGGAAGGCAGCGCCGCAGAGTCCACGGGCTAAGGACGACCAGAGGTTGCTGGGCTTGCTCAAGCA
GGCATGGCTGGAGAGCGGCGGTGTCTATGGCTACCGCAAGCTCACGCTGGACATGCGCGACCTGGGCGAGCGCTGCGGCAAGCATCGCGTTGCGCGGTTG
CTCAAGCTCGAAGGCCTGCGCTCGCAGACGGGCTACGGCCGCAGGCCTGGGCGGCGAGGCGGCAAGCCTGCAGTGGTCGCACCAAACCACCTGCAGCGCC
AGTTCAAGGTGGATGAGCCCAATCAGTCCTGGGTCACCGACATCACCTACATCCGAACCCACGAAGGCTGGCTGTACCTGGCGGTTGTGGTCGATCTGTT
CTCGCGCCAAGTAGTGGGCTGGTCCATGGGCAGCCGCATTGACACGAGCCTGGTCCTCGATGCACTGCTGATGGCGCTATGGCGCCGCCAGCCGCATGCG
CCTGTCATGGTTCATTCCGATCAGGGCTGTCAGTTCACCGGCCACGAGTGGCAGACCTTCCTGCGCGAGCACAACCTGGTCAGTAGCATGAGCCGGCGTG
GCAACTGCCACGACAACGCCGTGGCCGAGAGCTTCTTCCAACTGCTCAAGCGCGAACGCATCCGCAGGCGGGTCTACGAAACCCGTGACGATGCGCGAGC
GGACGTCTTCAACTACATTGAAATGTTCTACAACTCACAGCGCCGTCATTCATCGGCGGCGGGACTATCACCTGTAGAGTTCGAACAGCGACATTCCCAA
CGGCTCAAAAGTGTCTAGGAATGCCGGGGCGATTCACCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 68 | 346 | + | No |
Description : First part of the transposase
ORF sequence :
MTKQPYPEEFKIEAVKQITERGHRVADVSARIGVSQHSLYKWIKAYAVPAPERQMQTSQTEELRRLKAELKRVTEERDILKKAAAYFARQSG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 314 aa | 274 | 1218 | + | No |
Description : Second part of the transposase
ORF sequence :
TQARDRGARHPKKSRSVLCQAVRVRYAFIKQHEQEHSVRRMCKVMQVHPSGYYAWKAAPQSPRAKDDQRLLGLLKQAWLESGGVYGYRKLTLDMRDLGER
CGKHRVARLLKLEGLRSQTGYGRRPGRRGGKPAVVAPNHLQRQFKVDEPNQSWVTDITYIRTHEGWLYLAVVVDLFSRQVVGWSMGSRIDTSLVLDALLM
ALWRRQPHAPVMVHSDQGCQFTGHEWQTFLREHNLVSSMSRRGNCHDNAVAESFFQLLKRERIRRRVYETRDDARADVFNYIEMFYNSQRRHSSAAGLSP
VEFEQRHSQRLKSV
CGKHRVARLLKLEGLRSQTGYGRRPGRRGGKPAVVAPNHLQRQFKVDEPNQSWVTDITYIRTHEGWLYLAVVVDLFSRQVVGWSMGSRIDTSLVLDALLM
ALWRRQPHAPVMVHSDQGCQFTGHEWQTFLREHNLVSSMSRRGNCHDNAVAESFFQLLKRERIRRRVYETRDDARADVFNYIEMFYNSQRRHSSAAGLSP
VEFEQRHSQRLKSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 68 | 1218 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKQPYPEEFKIEAVKQITERGHRVADVSARIGVSQHSLYKWIKAYAVPAPERQMQTSQTEELRRLKAELKRVTEERDILKKSRSVLCQAVRVRYAFIKQ
HEQEHSVRRMCKVMQVHPSGYYAWKAAPQSPRAKDDQRLLGLLKQAWLESGGVYGYRKLTLDMRDLGERCGKHRVARLLKLEGLRSQTGYGRRPGRRGGK
PAVVAPNHLQRQFKVDEPNQSWVTDITYIRTHEGWLYLAVVVDLFSRQVVGWSMGSRIDTSLVLDALLMALWRRQPHAPVMVHSDQGCQFTGHEWQTFLR
EHNLVSSMSRRGNCHDNAVAESFFQLLKRERIRRRVYETRDDARADVFNYIEMFYNSQRRHSSAAGLSPVEFEQRHSQRLKSV
HEQEHSVRRMCKVMQVHPSGYYAWKAAPQSPRAKDDQRLLGLLKQAWLESGGVYGYRKLTLDMRDLGERCGKHRVARLLKLEGLRSQTGYGRRPGRRGGK
PAVVAPNHLQRQFKVDEPNQSWVTDITYIRTHEGWLYLAVVVDLFSRQVVGWSMGSRIDTSLVLDALLMALWRRQPHAPVMVHSDQGCQFTGHEWQTFLR
EHNLVSSMSRRGNCHDNAVAESFFQLLKRERIRRRVYETRDDARADVFNYIEMFYNSQRRHSSAAGLSPVEFEQRHSQRLKSV
Blast result :
Comments
ISAisp2 is 79% (ORFA) and 78% (ORFB) as similar to ISXac3. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., (2007) Direct submission GenBank