ISAhy1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP000462 ND Aeromonas hydrophila
Aeromonas hydrophila subsp. hydrophila ATCC 7966
DNA section
IS Length : 1032 bp

Ends


IR Length : 23/28

IRL : GGCTCTGTCCCAGTTATGTGTTGAAAGGCTAACCTTGAAGTAGGTCATCA
IRR : GGCTATGTCCCAATTACGTGTTGCATGGGGCGCCCCAGCGCCTCATGCAA

Insertion site


Left flankDirect repeatRight flankDR Length
CGAAGAGGGCAAATAAAAGGGAGGCCTC8
CATTGGGCGCTTTTTTTTTGCTTGAATT8

DNA sequence

GGCTCTGTCCCAGTTATGTGTTGAAAGGCTAACCTTGAAGTAGGTCATCACCTTGAGGGCCGCCATCATGGATACCCAAGCCTTCCAACATCTACTGTCC
CTCTTCCCATTGCTCACTTTGCGTCAGCGTCGTCTCGCGCAACAGGAACTCACCACCCCTCACCCCATCACCTCGTTGGCCACTCAACTGCCCGCATGCC
AATGCTGCCCTCACTGTCAGGCCGAGACCACACAGCTGGCTCCCTGGGGCTGGTCTCGAGGGCTGCGCCGTTATCGCTGCAAGCAGTGTTTGCGCACCAG
TTCCGTGCTCACCAACACGCCTCTCGCCCGACTTCGCAAGCCACAGTGCTGGGAAGACTATGCACAGGCCCTCATCGACGGTCTGACCGTCCGGCAGGCG
GCGCTCCGCTGTGGGGTCGGTAAAAACACCGCCTTTTTGTGGCGTCACCGCTTCCTGAAGGCCATGGCCAGCCACCAGGCGGTACGGGAAGAGGGTATCG
TCGAGGTCGATGAGACCTTCTTCCTGGCGTCGTTCAAAGGACAGCGCGGATTGCCCCGTCCGGCCCGCCATCGTGGAGGTAAAGGCCGTACCCGAGGCAC
AGGACCGGATTACATCCCGGTGATGGTGGTGCAAGACCGAGCAGGCTACCTGGCTGACTTCCAGCTGGAACGGCTGAATGCCCGGGCGGTGAAGGCGGTG
CTTGAGCCGCTTATCGCCCCGGATGCCGTCCTGTGCAGCGATGGTGCCGGGGTGTATGCGAGTTTCAGCGAGGAGCAAGGGATAACACACCAAGTGGTGC
GCAATCGAGCGGGAGAGCGGGTGGTGGGTGCATATCACATCCAACATGTGAACGGGTACCACAGGCGATTGAAGGAGTGGATGGAACGGTTCCATGGGGT
CGCGACCCACTATCTGAGGAATTATCTAGGTTGGCGAAGGATGTTGGAGCGCTATGGGCGGGAAGTGAATATCTCACGTTGCTTGCATGAGGCGCTGGGG
CGCCCCATGCAACACGTAATTGGGACATAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
963 bp320 aa681030+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDTQAFQHLLSLFPLLTLRQRRLAQQELTTPHPITSLATQLPACQCCPHCQAETTQLAPWGWSRGLRRYRCKQCLRTSSVLTNTPLARLRKPQCWEDYAQ
ALIDGLTVRQAALRCGVGKNTAFLWRHRFLKAMASHQAVREEGIVEVDETFFLASFKGQRGLPRPARHRGGKGRTRGTGPDYIPVMVVQDRAGYLADFQL
ERLNARAVKAVLEPLIAPDAVLCSDGAGVYASFSEEQGITHQVVRNRAGERVVGAYHIQHVNGYHRRLKEWMERFHGVATHYLRNYLGWRRMLERYGREV
NISRCLHEALGRPMQHVIGT

 

Blast result :
Comments
ISAhy1 is 66% aa similar to ISAzvi1. There are two copies in the genome of Aeromonas hydrophila subsp. hydrophila ATCC 7966.
References
1] Seshadri,R., Joseph,S.W., Chopra,A.K., Sha,J., Shaw,J., Graf,J., Haft,D., Wu,M., Ren,Q., Rosovitz,M., Madupu,R., Tallon,L., Kim,M., Jin,S., Vuong,H., Stine,O.C., Ali,A., Horneman,A.J. and Heidelberg,J.F. (2006) J. bacteriol.188(23), 8272-82.