ISAzo7

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 1044 bp

Ends


IR Length : 19/21

IRL : GGGGGTATCCACTTAGCTCCACCGACCCCGGAGTGCTGTATATTTATATA
IRR : AGGAGTATCCACTTAGCTCCATTAAGCCTGAGGTGGTCGCCGTTTCCGTG

Insertion site


Left flankDirect repeatRight flankDR Length
TATGAACTGTATTTTCGTACAGTCTTTC10

DNA sequence

GGGGGTATCCACTTAGCTCCACCGACCCCGGAGTGCTGTATATTTATATATATGATAGCTCCGGTTGCAGGGATGGACTACCCCCGAACGTGGAACGAGT
TCTTGGACTGGTTCCCGACGGAGGAGGCCTGCATGGCCTTTGTCGAGCGGCTTCGCTGGCCGCACGGTTTCACCTGTCCGGCGTGTGGGGTGGTCGAGCC
GCCGTAACGCGCCAGTCGGGGACGCTTGATGTGCCGCAGTTGCCGGCATCAGACGTCGGTCACGACCGGCACGATCTTCGAGAAGACGCGCACGCCGTTG
CGCGTATGGTTGGCGGGGGCTTGGTATGCAACCAACCAGAAGCTGGGCGTGAGTGCCCTCGGCTTGCAGCGGGTGCTTGGCCGAGGCAGTTACCAGACGG
CGTGGACGATGCTGCGCCGCTTTCGCCGAGCCATGGTGCGCCCGGAGCGCGAAAAACTGCATGGGCTGGTCGAAGTCGATGAAACCTATTTGGCCCTCAC
CGACCGGCAGTTACCCCTTACGGCCGCGGGGCGCAAGAGCAAGACCACCAAGGTCCTGGTCGTCATGGCGGTGGAAATCCACCAGCCGAAGGGGTTCGGG
CGAATTCGGCTGCGCCGGGTGAGCGACGATAGTGCTGCGAGCGTAATTCCGTTCGTTCAGGAAGCCGGCGACGCCGCGGCCGTCGTGCGTACCGACGGCT
CGGCTGCCTATCTGGGATTGAAGTCGCTCGGATATGGGCACGAGCGCAAAGTCATGCTCGGCGCGGAGGATGCGGCCCATGTCGCAATGCCCGGCGTCCA
CCGGGTCGCGTCGTTGGTGAAGCGGTGGATTCTCGGTACGCACCAAGGCTCAGTGCAGCCCGACCAGCTCGACTTCTACCTGGATGAATTCGTGTTCCGT
TTCAACCGCCGCGCCTCGCGCTCACGCGGCATGCTGTTCTACCGGCTGATGGAGCAGGCTGTGCGGACTCCACCTGTAACGTACGCCGATGTTGCACGGA
AACGGCGACCACCTCAGGCTTAATGGAGCTAAGTGGATACTCCT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
951 bp316 aa731023+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDYPRTWNEFLDWFPTEEACMAFVERLRWPHGFTCPACGVVEPP*RASRGRLMCRSCRHQTSVTTGTIFEKTRTPLRVWLAGAWYATNQKLGVSALGLQR
VLGRGSYQTAWTMLRRFRRAMVRPEREKLHGLVEVDETYLALTDRQLPLTAAGRKSKTTKVLVVMAVEIHQPKGFGRIRLRRVSDDSAASVIPFVQEAGD
AAAVVRTDGSAAYLGLKSLGYGHERKVMLGAEDAAHVAMPGVHRVASLVKRWILGTHQGSVQPDQLDFYLDEFVFRFNRRASRSRGMLFYRLMEQAVRTP
PVTYADVARKRRPPQA

 

Blast result :
Comments
ISAzo7 is 84% aa similar to ISPosp3. At position 45 of the transposase, a ponctual mutation generate a stop codon.
References
1] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36