ISAisp1

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008782 ND Acidovorax sp.
Acidovorax sp. JS42
DNA section
IS Length : 1086 bp

Ends


IR Length : 22

IRL : AGGGGTATCTATTTAGCTCCAGTGACGTGAGAATAGCTGGATGGAAACCC
IRR : AGGGGTATCTATTTAGCTCCAGCAGCCCCATTGATACTGTGATTTCATCC

Insertion site


Left flankDirect repeatRight flankDR Length
ATCATTGACTGTATAAACGCACAGTCTTTTGT10
TAACATGCCTGG/TATGCATTTACAGTGGCACGC10
GATGATTACTGTATATTTGAACAGTATTCATC10
CAGAATAACTGTTTATTTATACAGTTATTCAG10

DNA sequence

AGGGGTATCTATTTAGCTCCAGTGACGTGAGAATAGCTGGATGGAAACCCAGATGCTAACGCCAACTCCTGGAGGGGACTACCCCCGCACCTGGAACGAA
TTTCTTGACTGGTTTGCCACCGAAGAGGCTTGCCAGGCGTTCCTGGAGAAGCTTCGGTGGCCCCAAGGCTTCGTCTGCCCGCGTTGCGGCAACGCTGGCG
ATGTGTACCGCGCCAGCCGCACCCGCCTGATGTGCCGCTCATGCCAGTACCAAGGCACAGTGACATCTGGAACCATCTTTGACAAGACGCGCACACCGCT
GCGTGTCTGGCTGGCTGCAGCTTGGTACCTGACCAATCAGAAACAAGGCGTGAGCGCCCTGGGGCTGCAGCGCGTATTGGGTTTGGGGAGCTACCAGACC
GCCTGGACCATGCTGCACCGGTTTCGACGTGCCATGGTCCGCCCGGGCCGGGACAAGCTCAAGGGGCTTGTGGAGGTGGATGAAACGTACCTGTCCATCA
CGGATCGCAAGAATCCCGCCACCCCTGCAGGGCGCAAGAGCAGCACCACCAAAGTGTTGATGGTCATGGCGGTGGAGATCGTGGAGCCCAAGGGGTTTGG
GCGCATCCGGTTACGCCGCATTGACCGAGACGCCGCCACCCACGTAATCCCCTTTGTGCAGGAGGTGGTCGAGCCTGGAGCCCAGGTACGCACGGATGGT
TCGGCGGCATACCGCGCTCTGGGAGAACTGGGTTACACCCACCAGCGCACCGTCATGCTCGGCTCAGGCGTACCTGCCCATGTCTCCATGGCGGGAGTGC
ACCGGGTCGCCTCACTGGTACAGCGCTGGGTGCTGGGAACACATCATGGCTCTGTACAGCCAGACCACCTGGACGCCTATCTCGATGAATTCGTGTTCCG
TTTCAACCGGCGCACATCCAGCTCACGCGGGATGCTGTTTTACCGCTTGCTGCAGCAAGCGGTGGTTACGCCGCCAGTGACGTATGGGGATGTCGTGAGC
AAGAACACCGGGGAGAGCCAATCGGATACCATTGCTGGATGAAATCACAGTATCAATGGGGCTGCTGGAGCTAAATAGATACCCCT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1002 bp333 aa411042+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

METQMLTPTPGGDYPRTWNEFLDWFATEEACQAFLEKLRWPQGFVCPRCGNAGDVYRASRTRLMCRSCQYQGTVTSGTIFDKTRTPLRVWLAAAWYLTNQ
KQGVSALGLQRVLGLGSYQTAWTMLHRFRRAMVRPGRDKLKGLVEVDETYLSITDRKNPATPAGRKSSTTKVLMVMAVEIVEPKGFGRIRLRRIDRDAAT
HVIPFVQEVVEPGAQVRTDGSAAYRALGELGYTHQRTVMLGSGVPAHVSMAGVHRVASLVQRWVLGTHHGSVQPDHLDAYLDEFVFRFNRRTSSSRGMLF
YRLLQQAVVTPPVTYGDVVSKNTGESQSDTIAG

 

Blast result :
Comments
ISAisp1 is 88% aa similar to ISPosp3.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.