ISMyma3
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Mycobacterium marinum | Mycobacterium marinum M |
DNA section
IS Length : 1364 bp
Ends
IR Length : 22
IRL : TGACTCCTCCCGGCGTGTCCGGAGGCTCCTTGCGTTGGGAGAATCTGGGT
IRR : TGACTCCTCCCGGCGTGTCCGGGGACTCGGTTTCTTGAGTCACCCGGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGACTCCTCCCGGCGTGTCCGGAGGCTCCTTGCGTTGGGAGAATCTGGGTCATGGCACGTGCAAGCAAGTATCCCGAGGAGCTTCGGGAGCGGGCTGTTC
GGATGGTCGCTGAGATAGCACCGCAGTACGGGTCGCAGTGGGCTGCGATCTGCGCTGTGGCGGGCAAGCTGGGAGTGGGCACACCCGAGACGGTGAGGAC
GTGGGTGCGGCGCGCTGAGGTCGATGCCGGACAGCGCCCGGGTGTGAGCAGTGAACAGTCCGAGGAACTCAGGCGCCTCAAGCGCGAGAACGCCGAATTG
CGTAGGGCCAATGAGATTCTCAAAGCAGCAGCAATTTTCTTCGGGGCCGAGCTCGACCGGCCCGCGAGGCGGTAATTCGGTTTATCACCGAGCACAAGGA
TCACCAGACGCCCGGCCCAGACGGGGCCGGGCTGATCTGGGGAGTCGAGCCCCTGTGCGCCGTGCTCACTGAGCATGGCATCAAGATCAGCCCTTCCACC
TACTACGAGTGGATGAAAAGAACACCGAGCAAGCAGCAGCTTCGCGATGAAGCGGTGACCGCCTCGATCAAGGAATTCCGCGAACGCAACCCGTTGAACA
AGACCCTGGGCAGCCGCAAGGTATGGATCAAGCTGCGTGGGGACGGCATGGACGTTGCGCGATGCACCGTTGAGCGTCTGATGCAGGCCAACCGCTGGCA
GGGTGCCCGTTACGGTGGCGCGCCCAAAACGACCATCCAGGACGAGAAGGCCCAGCGGGCAACGGATCTCGTCGACCGCGTCTTCGCCGCGCCGGCCCCC
AACCGGTTGTGGGTGGCCGATTTCACGTATTGCCCGACATGGGCGGGGATGGTGTACGTAGCGTTCGTGATTGACGCGTTCGCCCGCCGGGTCATCGGCT
GGCGCGCAGCCACGTCCATGAAGACCGCGTTGGTTATAGATGCGCTCGAGCATGCGTTCTTCACCCGCGCACAGGAGGGCTTCACTGATCTGTCCGGGCT
GGTGGCGCACAGCGACGCCGGGTCGCAATACACGTCTATCGCGTTCACCACGCGGCTGATCGATGCTGGTATCGACGCCTCGGTCGGATCCGTGGCCGAC
GCGTATGACAACGCCCTCGCCGAGACCACCGTGGGGTCATTCAAGAACGAGCTGATCCGCCGGCAGGGGCCATGGCGCGATGTCGACCACGTCGAGATCG
GCACCCTGAACTGGGTTGACTGGTTCAACAATGAACGGCCCCACGAGTACCTCGCCGACCTCACCCCGACACAGGCGGAGGCATTGCACTACCGTCACAA
GAGTTCCCTGGCAGCAGCCGGGTGACTCAAGAAACCGAGTCCCCGGACACGCCGGGAGGAGTCA
GGATGGTCGCTGAGATAGCACCGCAGTACGGGTCGCAGTGGGCTGCGATCTGCGCTGTGGCGGGCAAGCTGGGAGTGGGCACACCCGAGACGGTGAGGAC
GTGGGTGCGGCGCGCTGAGGTCGATGCCGGACAGCGCCCGGGTGTGAGCAGTGAACAGTCCGAGGAACTCAGGCGCCTCAAGCGCGAGAACGCCGAATTG
CGTAGGGCCAATGAGATTCTCAAAGCAGCAGCAATTTTCTTCGGGGCCGAGCTCGACCGGCCCGCGAGGCGGTAATTCGGTTTATCACCGAGCACAAGGA
TCACCAGACGCCCGGCCCAGACGGGGCCGGGCTGATCTGGGGAGTCGAGCCCCTGTGCGCCGTGCTCACTGAGCATGGCATCAAGATCAGCCCTTCCACC
TACTACGAGTGGATGAAAAGAACACCGAGCAAGCAGCAGCTTCGCGATGAAGCGGTGACCGCCTCGATCAAGGAATTCCGCGAACGCAACCCGTTGAACA
AGACCCTGGGCAGCCGCAAGGTATGGATCAAGCTGCGTGGGGACGGCATGGACGTTGCGCGATGCACCGTTGAGCGTCTGATGCAGGCCAACCGCTGGCA
GGGTGCCCGTTACGGTGGCGCGCCCAAAACGACCATCCAGGACGAGAAGGCCCAGCGGGCAACGGATCTCGTCGACCGCGTCTTCGCCGCGCCGGCCCCC
AACCGGTTGTGGGTGGCCGATTTCACGTATTGCCCGACATGGGCGGGGATGGTGTACGTAGCGTTCGTGATTGACGCGTTCGCCCGCCGGGTCATCGGCT
GGCGCGCAGCCACGTCCATGAAGACCGCGTTGGTTATAGATGCGCTCGAGCATGCGTTCTTCACCCGCGCACAGGAGGGCTTCACTGATCTGTCCGGGCT
GGTGGCGCACAGCGACGCCGGGTCGCAATACACGTCTATCGCGTTCACCACGCGGCTGATCGATGCTGGTATCGACGCCTCGGTCGGATCCGTGGCCGAC
GCGTATGACAACGCCCTCGCCGAGACCACCGTGGGGTCATTCAAGAACGAGCTGATCCGCCGGCAGGGGCCATGGCGCGATGTCGACCACGTCGAGATCG
GCACCCTGAACTGGGTTGACTGGTTCAACAATGAACGGCCCCACGAGTACCTCGCCGACCTCACCCCGACACAGGCGGAGGCATTGCACTACCGTCACAA
GAGTTCCCTGGCAGCAGCCGGGTGACTCAAGAAACCGAGTCCCCGGACACGCCGGGAGGAGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 324 bp | 107 aa | 52 | 375 | + | No |
Description : First part of the transposase
ORF sequence :
MARASKYPEELRERAVRMVAEIAPQYGSQWAAICAVAGKLGVGTPETVRTWVRRAEVDAGQRPGVSSEQSEELRRLKRENAELRRANEILKAAAIFFGAE
LDRPARR
LDRPARR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1011 bp | 336 aa | 315 | 1325 | + | No |
Description : Second part of the transposase
ORF sequence :
DSQSSSNFLRGRARPAREAVIRFITEHKDHQTPGPDGAGLIWGVEPLCAVLTEHGIKISPSTYYEWMKRTPSKQQLRDEAVTASIKEFRERNPLNKTLGS
RKVWIKLRGDGMDVARCTVERLMQANRWQGARYGGAPKTTIQDEKAQRATDLVDRVFAAPAPNRLWVADFTYCPTWAGMVYVAFVIDAFARRVIGWRAAT
SMKTALVIDALEHAFFTRAQEGFTDLSGLVAHSDAGSQYTSIAFTTRLIDAGIDASVGSVADAYDNALAETTVGSFKNELIRRQGPWRDVDHVEIGTLNW
VDWFNNERPHEYLADLTPTQAEALHYRHKSSLAAAG
RKVWIKLRGDGMDVARCTVERLMQANRWQGARYGGAPKTTIQDEKAQRATDLVDRVFAAPAPNRLWVADFTYCPTWAGMVYVAFVIDAFARRVIGWRAAT
SMKTALVIDALEHAFFTRAQEGFTDLSGLVAHSDAGSQYTSIAFTTRLIDAGIDASVGSVADAYDNALAETTVGSFKNELIRRQGPWRDVDHVEIGTLNW
VDWFNNERPHEYLADLTPTQAEALHYRHKSSLAAAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1274 bp | 424 aa | 52 | 1325 | + | Yes |
Chemistry : DDE
ORF sequence :
MARASKYPEELRERAVRMVAEIAPQYGSQWAAICAVAGKLGVGTPETVRTWVRRAEVDAGQRPGVSSEQSEELRRLKRENAELRRANEILKAAAIFLRGR
ARPAREAVIRFITEHKDHQTPGPDGAGLIWGVEPLCAVLTEHGIKISPSTYYEWMKRTPSKQQLRDEAVTASIKEFRERNPLNKTLGSRKVWIKLRGDGM
DVARCTVERLMQANRWQGARYGGAPKTTIQDEKAQRATDLVDRVFAAPAPNRLWVADFTYCPTWAGMVYVAFVIDAFARRVIGWRAATSMKTALVIDALE
HAFFTRAQEGFTDLSGLVAHSDAGSQYTSIAFTTRLIDAGIDASVGSVADAYDNALAETTVGSFKNELIRRQGPWRDVDHVEIGTLNWVDWFNNERPHEY
LADLTPTQAEALHYRHKSSLAAAG
ARPAREAVIRFITEHKDHQTPGPDGAGLIWGVEPLCAVLTEHGIKISPSTYYEWMKRTPSKQQLRDEAVTASIKEFRERNPLNKTLGSRKVWIKLRGDGM
DVARCTVERLMQANRWQGARYGGAPKTTIQDEKAQRATDLVDRVFAAPAPNRLWVADFTYCPTWAGMVYVAFVIDAFARRVIGWRAATSMKTALVIDALE
HAFFTRAQEGFTDLSGLVAHSDAGSQYTSIAFTTRLIDAGIDASVGSVADAYDNALAETTVGSFKNELIRRQGPWRDVDHVEIGTLNWVDWFNNERPHEY
LADLTPTQAEALHYRHKSSLAAAG
Blast result :
Comments
I was unable to determine target site duplications for this IS as I did not have sequence before and after transposition.
ISMyma3 is 82% (ORF1) and 65% (ORF2) aa similar to IS987.
ISMyma3 is 82% (ORF1) and 65% (ORF2) aa similar to IS987.
References
1] Stinear Tim. (2006) direct submission.