ISPasp2
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005861 | ND | Parachlamydia sp. | Parachlamydia sp. UWE25 |
DNA section
IS Length : 1023 bp
Ends
IR Length : 16/17
IRL : ACCTGAACTTTGGGTTTAGGTGTTTAGGCTTAAACAAGATACATAAAATT
IRR : ACCTGAATTTTGGGTTTTTAGGCAGCCATTAACAATAAGCGATGCTGCTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACCCATGCT | TAATA | TGATATAAAA | 5 |
| TAAAAACTAA | TAAGA | AGCTTTTTAC | 5 |
DNA sequence
ACCTGAACTTTGGGTTTAGGTGTTTAGGCTTAAACAAGATACATAAAATTTGAATGACAAAAGACCTCAACATGATTTAAAACATTTGTTTTCCCAAACT
AATGTTTAATATGCATGAGAGGTCAAAATGGATTTAATCAAATTATATTGTAGTGTGGATGATTTTTGGAAGTCTTTTGAGAAGAAATGGAATAAACAGT
TAATTGATCATGGCAAAACAAAGCGAGGACCTCAGCCTGAATTATCGATACCAGAGATGATGACAATTGTCATTTTATTTCATCAATCAAATTATCGAAC
ATTTAAGCACTTTTATGGGTATGTTACCAAATACTTAGTTAAAGAATTTCCAAACTTAATTAGTTATAGCCGTTTTGTTTATTTAAAGAAGAACCTTTTT
GTTCCTCTATTTGCTTATCTTTTAGACAAAAGAGGAGAAATCACAGGAATTGCTTTTATTGATTCTACGTCTATAGATGTTTGCCACAATAAACGCATAA
AAAGAAACAAGGTCTTTAAAGGTTTAGCAAAGAGGGGAAAAACAACTTCAGGGTGGTTTTTCGGATTTAAATTGCATCTCATGATCAATGAGAAAGGAGA
AATTTTGGCTTTTCAGCTAACTCCTGGAAATGTCGCTGATGTTTCTATTGCCGAAACTTTGTCGAAAGGGATTTTTGGAAAATTATTTGGTGATAAGGGA
TATATTTCGAAAGAACTTTCAAAGAGGCTTTTGAAACAAGGTCTTGAACTATTTACTACCCTAAGATCGAATATGAAGCAGAATCTTATGAAGTTGACAG
ACAAAATTCTTCTTAGAAAACGAGCCATTATTGAAACGGTGAATGATCAACTAAAAAATATTTCTCAAATTGAACATACACGCCACAGAAATGCCGGAAA
TTTTTTAATTAATCTATTAGCTGGGATTGTTGCTTATACTCACCAACCTAAAAAGCCATCTATAAACTTAACAGAGCAGCATCGCTTATTGTTAATGGCT
GCCTAAAAACCCAAAATTCAGGT
AATGTTTAATATGCATGAGAGGTCAAAATGGATTTAATCAAATTATATTGTAGTGTGGATGATTTTTGGAAGTCTTTTGAGAAGAAATGGAATAAACAGT
TAATTGATCATGGCAAAACAAAGCGAGGACCTCAGCCTGAATTATCGATACCAGAGATGATGACAATTGTCATTTTATTTCATCAATCAAATTATCGAAC
ATTTAAGCACTTTTATGGGTATGTTACCAAATACTTAGTTAAAGAATTTCCAAACTTAATTAGTTATAGCCGTTTTGTTTATTTAAAGAAGAACCTTTTT
GTTCCTCTATTTGCTTATCTTTTAGACAAAAGAGGAGAAATCACAGGAATTGCTTTTATTGATTCTACGTCTATAGATGTTTGCCACAATAAACGCATAA
AAAGAAACAAGGTCTTTAAAGGTTTAGCAAAGAGGGGAAAAACAACTTCAGGGTGGTTTTTCGGATTTAAATTGCATCTCATGATCAATGAGAAAGGAGA
AATTTTGGCTTTTCAGCTAACTCCTGGAAATGTCGCTGATGTTTCTATTGCCGAAACTTTGTCGAAAGGGATTTTTGGAAAATTATTTGGTGATAAGGGA
TATATTTCGAAAGAACTTTCAAAGAGGCTTTTGAAACAAGGTCTTGAACTATTTACTACCCTAAGATCGAATATGAAGCAGAATCTTATGAAGTTGACAG
ACAAAATTCTTCTTAGAAAACGAGCCATTATTGAAACGGTGAATGATCAACTAAAAAATATTTCTCAAATTGAACATACACGCCACAGAAATGCCGGAAA
TTTTTTAATTAATCTATTAGCTGGGATTGTTGCTTATACTCACCAACCTAAAAAGCCATCTATAAACTTAACAGAGCAGCATCGCTTATTGTTAATGGCT
GCCTAAAAACCCAAAATTCAGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 292 aa | 128 | 1003 | + | No |
Chemistry : DDE
ORF sequence :
MDLIKLYCSVDDFWKSFEKKWNKQLIDHGKTKRGPQPELSIPEMMTIVILFHQSNYRTFKHFYGYVTKYLVKEFPNLISYSRFVYLKKNLFVPLFAYLLD
KRGEITGIAFIDSTSIDVCHNKRIKRNKVFKGLAKRGKTTSGWFFGFKLHLMINEKGEILAFQLTPGNVADVSIAETLSKGIFGKLFGDKGYISKELSKR
LLKQGLELFTTLRSNMKQNLMKLTDKILLRKRAIIETVNDQLKNISQIEHTRHRNAGNFLINLLAGIVAYTHQPKKPSINLTEQHRLLLMAA
KRGEITGIAFIDSTSIDVCHNKRIKRNKVFKGLAKRGKTTSGWFFGFKLHLMINEKGEILAFQLTPGNVADVSIAETLSKGIFGKLFGDKGYISKELSKR
LLKQGLELFTTLRSNMKQNLMKLTDKILLRKRAIIETVNDQLKNISQIEHTRHRNAGNFLINLLAGIVAYTHQPKKPSINLTEQHRLLLMAA
Blast result :
Comments
ISPasp2 is 70% aa similar to ISPlu6.
ISPasp2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 46 residues. The copy number in Parachlamydia sp. UWE25 is 2.
ISPasp2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 46 residues. The copy number in Parachlamydia sp. UWE25 is 2.
References
[1]Horn,M., Collingro,A., Schmitz-Esser,S., Beier,C.L., Purkhold,U., Fartmann,B., Brandt,P., Nyakatura,G.J., Droege,M., Frishman,D., Rattei,T., Mewes,H.W. and Wagner,M.(2004)Science 304,728-730