ISPasp1
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005861 | ND | Parachlamydia sp. | Parachlamydia sp. UWE25 |
DNA section
IS Length : 1018 bp
Ends
IR Length : 17
IRL : ACCTGAACTTTGGGTTTAGGTGTTTAGGCTTAAACAAGATACATAAAATT
IRR : ACCTGAACTTTGGGTTTCAAGCAGCAATAGCTACACAGAATTTCTCTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGTAAACTA | TACTA | AAATAGAATA | 5 |
DNA sequence
ACCTGAACTTTGGGTTTAGGTGTTTAGGCTTAAACAAGATACATAAAATTTGAATGACAAAAGACCTCAACATGATTTAAAAGCATTGTTTACCACAACC
ATACTTAATCATGAGAGGTCAAAATGGATGTAACTGAACTATATTGTACAATTGATGATTTCTGGAAATCTTTCAAGCAAGAATGGGAAAAACACCTTAT
AGATTCTGGAAAGCACAAAAAGGGCCCAGAATCTGTGTTATCTCCAGCTGAAATGATGACCATCATCGTTTTATTTCATCAATCAAATTACAGAACTTTC
AAACACTTCTATAACTACATTAATTTTTATTTAAGAAATGAATTTCCAAAATTAATAAGCTATGATCGATTCGTTTATTTAAAGAAAAATATGTTTGTTC
CTTTATTTGCTTACCTCATGCACAGAAAAGGTAAAGTGACGGGAATAGCTTTTATTGATTCTACATCAATAGCAGTATGTCATAATAAAAGAATTTCGCG
AAATAAGGTATTTGCAGGGCTAGCAAAAAGAGGGAAGAACACTTCAGGATGGTTTTACGGTTTCAAATTACACATAATTAATAATGACAGCGGGGAAATT
TTAGCTTTTCAACTTACACCAGGTAATGTATCTGATGTATCGATGGCTGAAACCCTTTCCAAGGGGCTGTTTGGAAAGTTATTTGGAGACAAAGGTTATA
TTTCTGGAAAATTAACAGAAAGTCTTCTAGAACAGGGCTTAGAGTTGTTTACGAGTGTACGAGCAAACATGAAACAAAAATTAATGACCTTTAATGATAA
AATTTTATTACGAAAGAGATCTCTTATAGAGACAGTCAACGATCAACTTAAAAATATTTCTCAAATTGAACATACTCGACATCGTAATATAGGCAATTTT
TTGGTAAATATGCTAGGTGGGCTTGCTGCATATAGCCATCAACGGAAAAAGCCGTCTTTAAATTTTAGAGAAGAGAAATTCTGTGTAGCTATTGCTGCTT
GAAACCCAAAGTTCAGGT
ATACTTAATCATGAGAGGTCAAAATGGATGTAACTGAACTATATTGTACAATTGATGATTTCTGGAAATCTTTCAAGCAAGAATGGGAAAAACACCTTAT
AGATTCTGGAAAGCACAAAAAGGGCCCAGAATCTGTGTTATCTCCAGCTGAAATGATGACCATCATCGTTTTATTTCATCAATCAAATTACAGAACTTTC
AAACACTTCTATAACTACATTAATTTTTATTTAAGAAATGAATTTCCAAAATTAATAAGCTATGATCGATTCGTTTATTTAAAGAAAAATATGTTTGTTC
CTTTATTTGCTTACCTCATGCACAGAAAAGGTAAAGTGACGGGAATAGCTTTTATTGATTCTACATCAATAGCAGTATGTCATAATAAAAGAATTTCGCG
AAATAAGGTATTTGCAGGGCTAGCAAAAAGAGGGAAGAACACTTCAGGATGGTTTTACGGTTTCAAATTACACATAATTAATAATGACAGCGGGGAAATT
TTAGCTTTTCAACTTACACCAGGTAATGTATCTGATGTATCGATGGCTGAAACCCTTTCCAAGGGGCTGTTTGGAAAGTTATTTGGAGACAAAGGTTATA
TTTCTGGAAAATTAACAGAAAGTCTTCTAGAACAGGGCTTAGAGTTGTTTACGAGTGTACGAGCAAACATGAAACAAAAATTAATGACCTTTAATGATAA
AATTTTATTACGAAAGAGATCTCTTATAGAGACAGTCAACGATCAACTTAAAAATATTTCTCAAATTGAACATACTCGACATCGTAATATAGGCAATTTT
TTGGTAAATATGCTAGGTGGGCTTGCTGCATATAGCCATCAACGGAAAAAGCCGTCTTTAAATTTTAGAGAAGAGAAATTCTGTGTAGCTATTGCTGCTT
GAAACCCAAAGTTCAGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 292 aa | 124 | 999 | + | No |
Chemistry : DDE
ORF sequence :
MDVTELYCTIDDFWKSFKQEWEKHLIDSGKHKKGPESVLSPAEMMTIIVLFHQSNYRTFKHFYNYINFYLRNEFPKLISYDRFVYLKKNMFVPLFAYLMH
RKGKVTGIAFIDSTSIAVCHNKRISRNKVFAGLAKRGKNTSGWFYGFKLHIINNDSGEILAFQLTPGNVSDVSMAETLSKGLFGKLFGDKGYISGKLTES
LLEQGLELFTSVRANMKQKLMTFNDKILLRKRSLIETVNDQLKNISQIEHTRHRNIGNFLVNMLGGLAAYSHQRKKPSLNFREEKFCVAIAA
RKGKVTGIAFIDSTSIAVCHNKRISRNKVFAGLAKRGKNTSGWFYGFKLHIINNDSGEILAFQLTPGNVSDVSMAETLSKGLFGKLFGDKGYISGKLTES
LLEQGLELFTSVRANMKQKLMTFNDKILLRKRSLIETVNDQLKNISQIEHTRHRNIGNFLVNMLGGLAAYSHQRKKPSLNFREEKFCVAIAA
Blast result :
Comments
ISPasp1 is 69% aa similar to ISPlu6.
ISPasp1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 46 residues. The copy number in Parachlamydia sp. UWE25 is 1.
ISPasp1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 46 residues. The copy number in Parachlamydia sp. UWE25 is 1.
References
1] Horn,M., Collingro,A., Schmitz-Esser,S., Beier,C.L., Purkhold,U., Fartmann,B., Brandt,P., Nyakatura,G.J., Droege,M., Frishman,D., Rattei,T., Mewes,H.W. and Wagner,M.(2004)Science 304,728-730