ISAtu2

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003305 ND Agrobacterium tumefaciens
Agrobacterium tumefaciens C58
DNA section
IS Length : 1353 bp

Ends


IR Length : 22

IRL : TCGTTTCTTCTCCCCGCCGGGGGCCGTTGCAAAATTCGACGATTGTGATT
IRR : TCGTTTCTTCTCCCCGCCGGGGAGAAGGTGGCGCGTAGCGCCGGATGAGG

Insertion site


Left flankDirect repeatRight flankDR Length
GAGAGTGCCGCCTGCAACCTC1

DNA sequence

TCGTTTCTTCTCCCCGCCGGGGGCCGTTGCAAAATTCGACGATTGTGATTCACTCCTGTCAGGTTGATGGGAGATGTTGGGATGTCGGTTCGCGACAGCA
GGCAGTTCAGTTTTGTCGAAGCGCTGATGCCGCAGGCGAAGGGTGCCGGCCGGCTTGAGCGTTTGTCGGGTGTGGTGAAGTGGTATCGGTTCGAGAAGCT
TCTTGCGCGTCTGCGCGACGACCAGGGCGCCGGTCGTCCGGCATACAAACCGCTGGTGATGTTCAAGGCGCTGCTGTTGCAGTCGCTTTATGGGCTTTCC
GACATGGAACTCGAGGAGGCGCTTGGCGACCGGCTGTCGTTCAAACGCTTTGTCGGGCTTGCGCTGGAGGAAACGGTTCCCGATCATTCCACGCTTTGCC
GCTTTCGCAATCTGCTGATCGGAAAAGACCTTCTGGAGAAGCTGTTTGGCGAACTCGACCGTCAACTCGATGCCGCCGGCCTGATCCTGCGACGCGGCAC
GATGCTGGACGCAACGATCATCGAAACGGCGGCGGCGCGACCGCCCAGCGGGCGCAATCGCGACCTCGATGAGGCGGCGGCCCTTGCGGATCCGCATGAG
CAGGAAGAGCATGGCCCTGCACACGATGCGGATGCCCGTTTCGTCCGCCACGCGGGTAAAACCGGCTCGGCCTATGGCTACAAGGCGCATGTCGGCGTCG
ATGAGGGCTCAGGCCTGATCCGCAGGATCATCACCACGCCGGCCAATGTCAACGACACCGTCGTCGCCGACATGCTGGTCGTGGGCGACGAGGCCTATGT
TCTGGCGGATGGGGCGTATCACACGCATGGACGGGAGAAAGAGCTGAAGGCGCGCGGGATCAAGCCGCGGCTGATGCGCCGGCCGAACCGGCATCATCGT
ATGCTGTCGCCGCGGCTTGCCCGCTTCAACCTGCTGATCTCGAGGCGTCGCTCAGCTGTCGAAACGACGTTCGCCACGCTGAAGAACCGGATGGGGCTTG
ATGTGATCCGCTACCGTGGACTTGCCAAGGCGACGGCCCAGGTGCTGCTGGCGGCGATGGCCTTCAACATGCGTCGCTGGGTGACTTTGACGACACCTGC
AAGGCCGCACTGCGCCTGAAAACCACCCAGAGCATCCTCAAACAACCCAAAGCCCGCCACAGACACAGGCGGGCCGGGAAAATGCGGCTGAAAACATCAG
GAGACTCTCAGGCGACGGATTACGCAACGGCCCCCGCCGGGGAGAAGATGCCCCGAAGTGGCAGATGAGGGGGCCAGCTCTCCGCATCTCTCTGCCGTCG
CCCCCTCATCCGGCGCTACGCGCCACCTTCTCCCCGGCGGGGAGAAGAAACGA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1053 bp350 aa671119+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGDVGMSVRDSRQFSFVEALMPQAKGAGRLERLSGVVKWYRFEKLLARLRDDQGAGRPAYKPLVMFKALLLQSLYGLSDMELEEALGDRLSFKRFVGLAL
EETVPDHSTLCRFRNLLIGKDLLEKLFGELDRQLDAAGLILRRGTMLDATIIETAAARPPSGRNRDLDEAAALADPHEQEEHGPAHDADARFVRHAGKTG
SAYGYKAHVGVDEGSGLIRRIITTPANVNDTVVADMLVVGDEAYVLADGAYHTHGREKELKARGIKPRLMRRPNRHHRMLSPRLARFNLLISRRRSAVET
TFATLKNRMGLDVIRYRGLAKATAQVLLAAMAFNMRRWVTLTTPARPHCA

 

Blast result :
Comments
ISAtu2 is 50% aa similar to ISSsp126.
ISAtu2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 50 residues. The copy number in Agrobacterium tumefaciens str. C58 is 1.
References
1] Wood DW, Setubal JC, Kaul R, Monks DE, Kitajima JP, Okura VK, Zhou Y, Chen L, Wood GE, Almeida NF Jr, Woo L, Chen Y, Paulsen IT, Eisen JA, Karp PD, Bovee D Sr, Chapman P, Clendenning J, Deatherage G, Gillet W, Grant C, Kutyavin T, Levy R, Li MJ, McClelland E, Palmieri A, Raymond C, Rouse G, Saenphimmachak C, Wu Z, Romero P, Gordon D, Zhang S, Yoo H, Tao Y, Biddle P, Jung M, Krespan W, Perry M, Gordon-Kamm B, Liao L, Kim S, Hendrick C, Zhao ZY, Dolan M, Chumley F, Tingey SV, Tomb JF, Gordon MP, Olson MV, Nester EW (2001)Science 294,2266