ISfinder Mirror Site

ISC1212

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002754	ND	Sulfolobus solfataricus	Sulfolobus solfataricus P2

DNA section

IS Length : 1213 bp

Ends

IR Length : 19/24

IRL : GACGTTATCCAAGTTGATTAAAATGGCAATTCATGAGTAATATTAATAAG
IRR : GACGTTGTCCGATTTGAGCAAAATTAAAAGCCATTATAATTACAACGTCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTCCTTCCTC	ACGT	CACAAGTCCT	4

DNA sequence

GACGTTATCCAAGTTGATTAAAATGGCAATTCATGAGTAATATTAATAAGAAATTAGGATGAGATAATATTATGGTAAAGGCAATTTCAACTGAAAAGGA
CCTCTTGCTCAAGGTAGATAAATCCTTCCCTTGGGAAACGTTTAGGAGTAAGCTTAAGTCCCTTTACTCCAAGAAGCCCAAGTGGAACGTCATCTCACTC
CTCAAAGTCCTCCTAATCAAGCTCATTTTCGACATCTCCTGGAATAACTTGGAGGGAGAAATTAGGGACAGTAAGAGGTTTATGGACTTCTTGGGCGGGA
AAATTCCACCAAAGAGCACAGTATTCTCCTTCTACAAGAAACTTCAACAAACAGTTATTCAAGAAGGCGAGACGATGAGAACAACACTAATGGATGAGTT
AAACAAGGCTTTGGACAAGGTGATCAGCGAGTATAGGGAAAAGGGCTTTGAACTTGAAGTTGGGAGAGAAAAAACGATAGGTTCTAGGACTACCACATAA
TTGACACATTCTTCCGTGAGGCCTACCCAGGAAAGAGGAGTCTTCAAAACGGTCTTGAGAAGATCTCACTTGACCTTAAGGAGATGGGGTTCAATGACGT
TTTTCTCCTCATGGGTTATACTACTTCCAAGCTCAAGAACTTCACAGCCTTTAGGAAGTATAAGGGTAGTTGGGGTAGGAAGCACGGTAAGTCCTACTTC
GGGTTTAAGGTCTGTAACCTTGTGGAAAGGAGGACTAATTTCGTTAGGGGCTTCAAGGTTGGATTGGCTAATTTGAGTGATCTAGCCTTTTCATTTGATA
ACGTTAAGTTAATGGCTGATAGGGCTTGGATTTCCAGGAAGGACGTCTTAGTTAAGGGTGTTGGTAGTGCTAGACTTCCGGTTGAGGGTAGTGGTGTTAA
GATTAGGGAAGGTAAGGCTTCGTCTACAACCTTAAGGGGAGTGGTTATGGAGGTATTCTTCCTTAACCTCTATCGTGATCTCGAAATTCTTTCGACGAGG
ATTAGGACGAAGGTACTCGTTAATTAAACGAGGTTCGTTTACGTGTTTACTTGTTGTTATGATAAATTTTATTGCAAAACTGTACAATTATCTGTATTTT
CCCTTATTAATACATATTATATTATTTTTTATACTTGAATATAATGAAGAATAGTATAATATGAGACGTTGTAATTATAATGGCTTTTAATTTTGCTCAA
ATCGGACAACGTC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
429 bp	142 aa	72	500	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVKAISTEKDLLLKVDKSFPWETFRSKLKSLYSKKPKWNVISLLKVLLIKLIFDISWNNLEGEIRDSKRFMDFLGGKIPPKSTVFSFYKKLQQTVIQEGE
TMRTTLMDELNKALDKVISEYREKGFELEVGREKTIGSRTTT

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
444 bp	147 aa	584	1027	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MGFNDVFLLMGYTTSKLKNFTAFRKYKGSWGRKHGKSYFGFKVCNLVERRTNFVRGFKVGLANLSDLAFSFDNVKLMADRAWISRKDVLVKGVGSARLPV
EGSGVKIREGKASSTTLRGVVMEVFFLNLYRDLEILSTRIRTKVLVN

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
956 bp	0 aa	72	1027	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

Blast result :

Comments

ISC1212 is 47%(ORF1) aa similar to ISMbu1 and 57% (ORF2) to ISSto3.

References

1] She,Q., Singh,R.K., Confalonieri,F., Zivanovic,Y., Allard,G., Awayez,M.J., Chan-Weiher,C.C., Clausen,I.G., Curtis,B.A., De Moors,A., Erauso,G., Fletcher,C., Gordon,P.M., Heikamp-de Jong,I., Jeffries,A.C., Kozera,C.J., Medina,N., Peng,X., Thi-Ngoc,H.P., Redder,P., Schenk,M.E., Theriault,C., Tolstrup,N., Charlebois,R.L., Doolittle,W.F., Duguet,M., Gaasterland,T., Garrett,R.A., Ragan,M.A., Sensen,C.W. and Van der Oost,J. (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (14), 7835-7840